An <i>in vitro</i> evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates

Spratt, DA; Pratten, Jonathan; Wilson, Michael; Gulabivala, K

doi:10.1046/j.1365-2591.2001.00392.x

Cited by 230 publications

(232 citation statements)

References 41 publications

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“…NaOCl with different concentrations lead to contradictory results. For instance, in the study by Spratt et al, using NaOCl 3% resulted in complete elimination of E. faecalis 63 which is contrary to the results of the present study.…”

Section: Anti-bacterial Effect Of Er:yag Laser On Enterococcus Faecaliscontrasting

confidence: 99%

The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

et al. 2017

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Introduction: Successful root canal therapy depends on the complete elimination of microorganisms such as Entroccocus faecalis, which is impossible to achieve with the traditional methods. Lasers are recently introduced as a new method to solve the problem. The present study is planned and performed to examining the antibacterial effect of Er: YAG laser. Methods: Sixty extracted anterior primary teeth were prepared and sterilized. E. faecalis bacterium was cultured in canals. Samples were randomly divided into two groups. The first group was disinfected by NaOCl 5/25% and Er: YAG laser and the second group just by NaOCl 5/25%. Samples of canal contents were cultured and colony counts were calculated. The results were analyzed statistically by SPSS software and Mann Whitney test. Results: There was no significant difference between colony counts in both groups (P = 0.142). But the number of colonies in the first group was lower than in the second group. Conclusion: Although, Er: YAG laser cannot completely eliminate E. faecalis bacterium, its simultaneous use with NaOCl decreases E. faecalis.

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Section: Anti-bacterial Effect Of Er:yag Laser On Enterococcus Faecaliscontrasting

confidence: 99%

The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

et al. 2017

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“…faecalis is an important opportunistic pathogen having the ability to adhere to biotic and abiotic surfaces allowing the formation of a biofilm that permits to grow and survive in harsh environments prevailing in the root canal (12,14,17). A number of infections caused by E.faecalis strains have proven to be particularly difficult to treat with current antibiotic therapies, partly owing to their intrinsic antibiotic resistance elements, capacity of growing in a biofilm (24). It has gained notoriety as a persistent organism that can survive as a monoculture in root canals (20).…”

Section: Discussionmentioning

confidence: 99%

Effects of Myrcia ovata Cambess. essential oil on planktonic growth of gastrointestinal microorganisms and biofilm formation of Enterococcus faecalis

Cândido¹,

Portella²,

Laranjeira³

et al. 2010

Braz. J. Microbiol.

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“…All the irrigants used are known to have antimicrobial properties however; they have differing efficacies with respect to different microbiai species. Although sodium hypochlorite (NaOCl) has been shown to totally eradicate E. IaecaLis either as a planktonic form or as biofilm (34). It might also be advisable to use chlorhexidine and iodine for irrigation as several in vitro studies have reported ability of these irrigants to eradicate t.Iaecalis (34,35,47).…”

Section: Discussionmentioning

confidence: 99%

“…Although sodium hypochlorite (NaOCl) has been shown to totally eradicate E. IaecaLis either as a planktonic form or as biofilm (34). It might also be advisable to use chlorhexidine and iodine for irrigation as several in vitro studies have reported ability of these irrigants to eradicate t.Iaecalis (34,35,47). These results !lOwever may be confounded by the variation in root canal anatomy and polymicrobial root canal flora.…”

Section: Discussionmentioning

confidence: 99%

Occurance of enterococci in root canals and in the immediate clinical environment

Aziz

Baharin

Spratt

et al. 2009

ADUM

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This study aimed to detect the presence of enterococci in the root canals of untreated and treated teeth with periapical disease and to compare this to their presence in the saliva and in the immediate surgical environment during root canal treatment.Using an aseptic technique, 33 samples were obtained from 27 untreated and 6 previously treated teeth associated with apical periodontitis. Reduced Transport Fluid (RTF) was used as transport medium. Saliva samples and areas in the surgical environment were also sampled. These were performed prior to chemo-mechanical debrjdement and obturation for every case. The saliva was diluted to 10-J and was plated on Bile Aesculin Azide (BEA) agar whereas the rest of the samples were plated on Bile Aesculin (BE) agar. These plates were then incubated aerobically at 37°C for 48 hours. All the colony types that blackened the agar were sub-cultured to obtain pure isolates and tested on 6.5% sodium chloride (NaCI). Growth on this medium was Gram stained for further confirmation of cell morphology. Gram positive cocci isolated from previous positive test were identified as enterococci. Enterococci were recovered from untreated cases only; from 2 teeth (in 2 patients) prior to chemo-mechanical debridement, from 3 teeth (in 3 patients) prior to obturation and I from saliva sample. A 'total of 5 samples from 5 different patients were positive for enteroco·cci. Sampling in the immediate surgical environment revealed a low occurence in the range of3.0% (1/33) to 15_2% (5/33). In conclusion, the occurence of enterococci in patients and the immediate surgical environment was low number.

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An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates

Cited by 230 publications

References 41 publications

The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

Effects of Myrcia ovata Cambess. essential oil on planktonic growth of gastrointestinal microorganisms and biofilm formation of Enterococcus faecalis

Occurance of enterococci in root canals and in the immediate clinical environment

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