An “Off-On” Type UTP/UDP Selective Fluorescent Probe and Its Application to Monitor Glycosylation Process

Abstract

A New fluorescent sensor based on a perylene-dpa-Zn platform was synthesized. Selective "Off-On" type fluorescence changes were observed upon the addition of UTP and UDP, which was also applied to monitor glycosylation processes.

Uridine 5′-Diphosphate Induces Chemokine Expression in Microglia and Astrocytes through Activation of the P2Y6 Receptor

“…Cortical slices were placed in a 96-well plate (one slice per well) filled with 80 ml HEPES-buffered solution (130 mM NaCl, 5 mM KCl, 1.5 mM CaCl 2 , 1 mM MgCl 2 , 10 mM glucose, and 10 mM HEPES [pH 7.4]) immediately after slicing and incubated with or without ectonucleotidase inhibitor (10 mM dipyridamole) for 10 min to 1 h. The levels of UDP secreted into the solution were measured using a fluorescence probe based on a perylene-dpa-Zn platform (Probe-1) provided by Dr. Juyoung Yoon (Department of Chemistry and Nano Science, Ewha Woman's University, Suwon, Korea) (35). HEPES-buffered solution (80 ml) was mixed with Probe-1 (10 mM), and fluorescence levels were measured at 485/535 nm (excitation/emission) with a Victor3 fluorescence reader (PerkinElmer).…”

“…HEPES-buffered solution (80 ml) was mixed with Probe-1 (10 mM), and fluorescence levels were measured at 485/535 nm (excitation/emission) with a Victor3 fluorescence reader (PerkinElmer). Probe-1 has greater specificity for UDP than UTP and has little specificity for ATP, ADP, AMP, UMP, or GTP (35).…”

“…First, we analyzed the levels of UDP secreted from cortical slices using Probe-1. This probe specifically detects UDP and UTP (with higher sensitivity for UDP) and not other nucleotides, including ATP, ADP, and UMP/uridine (35). Within 10 min after slicing, ∼6.4 6 3.5 mM UDP was detected, and the levels increased to 21.7 6 4.7 mM after 1 h (Table I).…”

Delivery modulation in silica mesoporous supports via functionalization in the pore outlets with a Zn(II)–bis(2-pyridylmethyl)amine complex

“…In fact the interaction of Zn(II)-dipicolylamine complexes with both organic and inorganic phosphates is well documented and for instance has been extensively used for the preparation of selective fluorogenic probes for these anions. [68][69][70][71][72][73][74][75][76][77] Moreover, in order to test the effect of the concentration of the anions on the [Ru(bipy) 3 ] 2+ dye release behavior of the SZn material additional experiments were carried out using anions at a concentration of 1.0 x 10 -2 mol L -1 . In this case nearly the same inhibition trend for solid SZn was observed (vide infra).…”

“…In fact phosphate has been reported to display a high affinity for Zn(II)-3-like complexes. [68][69][70][71][72][73][74][75][76][77] Moreover the effect of the anion size (and charge, vide infra) can be observed when the anion series phosphate, pyrophosphate, AMP, ADP or ATP is compared. In this case all these anions have been reported to form complexes with Zn(II)-3-like complexes and the different cargo release is related with their different size; i.e.…”