An immunocytochemical study of cat retinal Müller cells in culture

Lewis, Geoffrey P.; Kaska, Deborah D.; Vaughan, Dana K.; Fisher, Steven K.

doi:10.1016/0014-4835(88)90068-1

Cited by 37 publications

(20 citation statements)

References 43 publications

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“…GFAP was not detected by immunocytochemistry, indicating the absence of astrocytes (Schnitzer, 1987). GFAP can sometimes be de-tected in cultured Muller cells (Lewis et al, 1988;Mascarelli et al, 1991), but the culture method used in the present work seems to avoid GFAP expression (Hicks and Courtois, 1990). As Thy-1 can also be expressed by fibroblasts, we have tested the antibodies on a rat fibroblastic cell line.…”

Section: Discussionmentioning

confidence: 86%

Rat retinal müller cells express Thy‐1 following neuronal cell death

Dabin

Barnstable

1995

Glia

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In the normal rat retina the Thy-1 antigen is a specific marker of ganglion cells, but degeneration of ganglion cells in vivo does not remove completely the expression of Thy-1 in the retina. To reconcile these differences we have postulated that ganglion cell death could induce a glial response including the expression of Thy-1 in Müller cells, the main glial cell type in the retina. Using immunocytochemistry, we have shown that pure cultures of Müller cells were strongly labelled with antibodies against Thy-1. PCR amplification of cDNA reverse transcribed from Müller cell RNA indicated the presence of Thy-1 transcripts. Double labelling experiments with anti-Thy1 and anti-glutamine synthetase, a marker of Müller cells, indicated the presence of both antigens in the same cells. Although Müller cells expressed Thy-1 mRNA and protein when cultured in the absence of neuronal cells, when co-cultured with retinal neurons they were not labelled with antibodies against Thy-1. Our results suggest that Thy-1 is expressed by Müller cells following loss of retinal neurons. Thy-1 may have an important function during glial response to neuron death in retina.

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Section: Discussionmentioning

confidence: 86%

Rat retinal müller cells express Thy‐1 following neuronal cell death

Dabin

Barnstable

1995

Glia

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“…Their eyeballs were removed, and Müller cells were isolated by a technique described previously [14] and cultured in a dish with 10 ml of Dulbecco's Modified Eagle Medium (Sigma Chemical Company). Müller cells were immunocytochemically identified by two specific markers, anti-glutamine synthetase monoclonal antibody (Chemicon, Temecula, Calif., USA) and anti-carbonic anhydrase II polyclonal antibody (Rockland, Gilbertsville, Pa., USA) [21].…”

Section: Methodsmentioning

confidence: 99%

Triamcinolone acetonide suppresses interleukin-1 beta-mediated increase in vascular endothelial growth factor expression in cultured rat Müller cells

Itakura

Akiyama

Hagimura

et al. 2005

Graefe's Arch Clin Exp Ophthalmo

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“…Mü ller Glial Cell Culture-Mü ller glial cells were cultured from adult white rabbits following the procedure described previously by Edwards et al (24), which was modified from the method of Lewis et al (25). Animals were killed by intravenous sodium pentobarbital (100 mg/kg).…”

Section: Preparation Of [mentioning

confidence: 99%

Apolipoprotein E Is Synthesized in the Retina by Müller Glial Cells, Secreted into the Vitreous, and Rapidly Transported into the Optic Nerve by Retinal Ganglion Cells

Amaratunga

Abraham

Edwards

et al. 1996

Journal of Biological Chemistry

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We have investigated the synthesis and transport of apoE, the major apolipoprotein of the central nervous system, in the retina of the living rabbit. Four hours after the injection of [ ]Met/ Cys-labeled apoE is rapidly transported into the optic nerve and its terminals in the lateral geniculate and superior colliculus within 3-6 h in two distinguishable vesicular compartments. Mü ller glia in cell culture also synthesize and secrete apoE. Taken together, these results suggest that apoE is synthesized by Mü ller glia and secreted into the vitreous. ApoE is also internalized by retinal ganglion cells and/or synthesized by these cells and rapidly transported into the optic nerve and brain as an intact molecule. We discuss the possible roles of retinal apoE in neuronal dynamics.ApoE, a 36-kDa glycoprotein, is a component of a number of circulating plasma lipoproteins, including very low density lipoproteins, high density lipoproteins, and chylomicron remnants (1). Its primary function appears to be that of a recognition ligand for the receptor-specific removal of cholesteryl ester-rich lipoproteins from the circulation (2, 3). It also plays a role in local transport of cholesterol, as seen in nerves of both peripheral (4, 5) and central nervous systems (reviewed in Ref. 6) and has been implicated in mediating immune responses and cell proliferation, processes that may not be related to its association with lipid (for review, see Ref. 1).In plasma, apoE transports lipoproteins containing cholesterol, triglycerides and other lipids to various cells via binding to the low density lipoprotein (LDL) 1 receptor (7) or the LDL receptor-related protein (LRP)/␣ 2 -macroglobulin receptor (8, 9). In the central nervous system, apoE is primarily synthesized by the major glial cell, the astrocyte, in rodents and humans (10, 11) and is found in significant quantities in the cerebrospinal fluid (10). Since apolipoprotein B, the other molecule that can mediate the internalization of lipoproteins via association with the LDL receptor, is not synthesized in the central nervous system (reviewed in Ref. 6), it is highly likely that apoE plays a major role in cholesterol and lipid transport in this compartment.We have employed the in vivo rabbit retina to probe the synthesis, intracellular transport, and metabolism of central nervous system proteins including kinesin, the motor for anterograde rapid transport (12, 13), and the ␤-amyloid precursor protein (APP) (14, 15). Since it has been shown recently that individuals having the ⑀4 allele of apoE are at high risk for Alzheimer's disease (16), we decided to use this widely accepted model of normal adult retinal protein synthesis and metabolism (e.g. Refs. 17 and 18) to examine the synthesis and transport of apoE. In this report, we present evidence, obtained by the injection of [ 35 S]methionine/cysteine into the vitreous chamber that overlies the retina, that apoE is synthesized in significant quantities in vivo by rabbit Mü ller cells, the predominant glial cell of the retina. ApoE ...

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An immunocytochemical study of cat retinal Müller cells in culture

Cited by 37 publications

References 43 publications

Rat retinal müller cells express Thy‐1 following neuronal cell death

Rat retinal müller cells express Thy‐1 following neuronal cell death

Triamcinolone acetonide suppresses interleukin-1 beta-mediated increase in vascular endothelial growth factor expression in cultured rat Müller cells

Apolipoprotein E Is Synthesized in the Retina by Müller Glial Cells, Secreted into the Vitreous, and Rapidly Transported into the Optic Nerve by Retinal Ganglion Cells

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