An immunocytochemical study of the morphological reaction of nerves containing calcitonin gene-related peptide to microabscess formation and healing in rat molars

Taylor, Patrick E.; Byers, Margaret R.

doi:10.1016/0003-9969(90)90029-a

Cited by 80 publications

(50 citation statements)

References 30 publications

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“…The culture medium was changed every 2 days. The time intervals for cavity preparation were chosen to simulate those described in previous experiments on both human (Marsland and Shovelton, 1957) and rat (Taylor and Byers, 1990) teeth in vivo. These intervals are further corroborated by the healing process encountered after the application of calcium hydroxide (Schroder and Granath, 1971;Yamamura, 1985).…”

Section: Specimen Collectionmentioning

confidence: 99%

An in vitro Model of Human Dental Pulp Repair

Magloire¹,

Joffre²,

Bleicher³

1996

J Dent Res

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Abstract. Pulp tissue responds to dentin injury by laying down reactionary dentin secreted by existing odontoblasts or reparative dentin elaborated by odontoblast-like cells that differentiated from precursor cells in the absence of inner dental epithelium and basement membrane. Furthermore, growth factors or active dentin matrix components are fundamental signals involved in odontoblast differentiation. In vitro, dental pulp cells cultured under various conditions are able to express typical markers of differentiation, but no culture system can re-create pulp response to dentin drilling. This paper reports the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation (BrdU-positive cells), neovascularization (positive with antitype-IV collagen antibodies), and the presence of functional (3H proline-positive) cuboidal cells close to the injured area.After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. This tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.

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Section: Specimen Collectionmentioning

confidence: 99%

An in vitro Model of Human Dental Pulp Repair

Magloire¹,

Joffre²,

Bleicher³

1996

J Dent Res

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“…Cavity preparation or pulp exposure has been known to induce considerable sprouting of sensory nerve fibers (KIMBERLY and BYERS, 1988;TAYLOR et al, 1988;SATO, 1989;BYERS et al, 1990;TAYLOR and BYERS, 1990). A role for reparative dentin formation and pulp survival by neuropeptides has been reported (TAYLOR and BYERs,1990;BYERS and TAYLOR, 1993).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Localizations of Class II Antigens and Nerve Fibers in Human Carious Teeth: HLA-DR Immunoreactivity in Schwann Cells.

Yoshiba

Iwaku

et al. 1998

Archives of Histology and Cytology

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“…However, there is no available data on how dendritic cells are involved with the odontoblast differentiation in the regenerative process following direct capping with TCP containing 3Mix on the infected pulp. Some investigations have suggested that neural elements -including calcitonin gene-related peptide (CGRP) -influence the degree of tissue damage and pulpal healing after tooth injury; much more extensive reinnervation causes the regeneration of odontoblast-like cells (Kvinnsland et al, 1991;Byers et al, 1992) since that protein is capable of enhancing wound healing and promoting fibroblast proliferation (Taylor and Byers, 1990;Byers and Taylor, 1993;Trantor et al, 1995). This study aims to clarify the responses of neural elements and immune cells to antimicrobials in the regenerative process following direct capping with TCP containing 3Mix on the infected pulp.…”

Section: Introductionmentioning

confidence: 99%

<b>Responses of infected dental pulp to αTCP-containing antimicrobials in rat molars</b>

Sato

Kenmotsu

Nakakura-Ohshima

et al. 2011

Archives of Histology and Cytology

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. Subsequently, the exposed pulp was covered with TCP or TCP containing 3Mix, followed with glass ionomer cement. A pulp abscess lacking both dendritic cells and PGP 9.5-reactive nerve fibers was induced after pulp capping with TCP; in contrast, numerous dendritic cells accumulated along the pulp-dentin border followed by the differentiation of odontoblast-like cells and matrix deposition after the application of TCP containing 3Mix. PGP 9.5-reactive nerve fibers were also densely distributed and surrounded the accumulated dendritic cells in the medial dental pulp beneath TCP containing 3Mix. The findings indicate that the application of TCP containing 3Mix to the infected pulp induces an intense accumulation of dendritic cells, suggesting that these cells play crucial roles in the differentiation of odontoblast-like cells under pathological conditions. IntroductionIn carious or traumatic pulpal exposures, dental pulp is easily infected and the bacteria may survive within the dentinal tubules or pulp tissue even after treatment. In addition, it has been reported that bacteria of carious dentin can invade the pulp tissue through dentinal tubules without pulpal exposure by the carious process (Hoshino et al., 1992). Bacterial flora of humans in carious dentin (Hoshino, 1985), dental plaque (Hoshino et al., 1989), and necrotic pulp (Sato et al., 1993b) have been reported to consist mainly of obligate anaerobes. A mixture of ciprofloxacin, metronidazole, and minocycline or cefaclor Responses of infected dental pulp to αTCP-containing antimicrobials in rat molars S u m m a r y . -t r i c a l c i u m p h o s p h a t e ( T C P ) w i t hthe addition of antimicrobials such as ciprofloxacin, metronidazole, and cefaclor (3Mix) has been applied to sterilize the infected dentin and pulp in vivo. Both clinical and animal experiments have shown that 3Mix is effective for sterilizing infected tissues. However, the responses of the infected dental pulp to 3Mix remain to be fully determined at the cellular level. This study aims to clarify the responses of neural elements and immune cells to antimicrobials during the healing process of infected pulp using immunohistochemistry for protein gene product (PGP) 9.5 and class II major histocompatibility complex molecules using both light and electron microscopy. An artificial pulp exposure was prepared on the maxillary

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An immunocytochemical study of the morphological reaction of nerves containing calcitonin gene-related peptide to microabscess formation and healing in rat molars

Cited by 80 publications

References 30 publications

An in vitro Model of Human Dental Pulp Repair

An in vitro Model of Human Dental Pulp Repair

Immunohistochemical Localizations of Class II Antigens and Nerve Fibers in Human Carious Teeth: HLA-DR Immunoreactivity in Schwann Cells.

<b>Responses of infected dental pulp to αTCP-containing antimicrobials in rat molars</b>

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