An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses

Abstract

A possible antigenic relationship between the porcine enteropathogenic coronavirus-like agent (CVLA) and 6 known coronaviruses was examined by immunoelectron microscopy (IEM) and by immunofluorescence (IF). CVLA did not show cross reactivity with infectious bronchitis virus, transmissible gastroenteritis virus (TGEV), canine coronavirus (CCV) hemagglutinating encephalomyelitis virus (HEV), neonatal calf diarrhea coronavirus (NCDCV) or feline infectious peritonitis virus (FIPV). Antigenic relationship was detected by IEM between TGEV and CCV, NCDCV and HEV and by IF between TGEV and CCV, TGEV and FIPV, HEV and NCDCV.

The Biology of Coronaviruses

“…(Collins et al, 1982;Hasony & Macnaughton, 1981, 1982Schmidt & Kenny, 1981, 1982Yaseen & Johnson-Lussenburg, 1981). Studies on the antigenic relationships of coronaviruses present a complex pattern, but the data suggest that the family can be divided into four distinct groups, and these are shown in Table 2 (Gerna et al, 1981 ;Horzinek et al, 1982;Macnaughton, 1981;Maru & Sato, 1982;Pedersen et al, 1978;Pensaert et al, 1981;Reynolds et al, 1980;Schmidt & Kenny, 1981).…”

Coronaviruses in poultry and other birds

“…They have been assigned to three groups. Initially this was based on antigenic relationships (McKintosh et al ., 1969;Bradburne, 1970;Pensaert et al ., 1981), which were later largely supported by gene sequencing. Some 25 years ago it was suggested that TCoV was closely related to BCoV of Group 2 (Dea et al ., 1990).…”

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

“…failed to detect any antigenic cross-reactivity between PEDV and other coronaviruses (Pensaert et al, 1981;Kusanagi et al, 1992). However, some cross-reactivity has been detected with feline infectious peritonitis virus (FIPV;Yaling et aL, 1988).…”