An immunohistochemical evaluation of the proteins <scp>W</scp>nt1 and glycogen synthase kinase (<scp>GSK</scp>)‐3β in invasive breast carcinomas

Mylona, Eleni; Vamvakaris, Ioannis; Giannopoulou, Ioanna; Theohari, Irene; Papadimitriou, Christos; Keramopoulos, Antonios; Nakopoulou, Lydia

doi:10.1111/his.12095

Cited by 12 publications

(16 citation statements)

References 30 publications

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“…These results confirm that Wnt1 is up-regulated in breast cancer, and that it is not involved with a more invasive breast tumour type; both findings confirming those of previous studies [9][10][11]21,22]. EpithelialCatenin and sFRP4 revealed no significant difference between tumour types and also between normal and tumour tissue.…”

Section: Discussionsupporting

confidence: 89%

“…It is well established that Wnt1 is involved in breast cancer [9][10][11]; however, this study has revealed that Wnt1 expression does not differ with breast cancer invasiveness, showing that Wnt1 plays a role in a breast tumour phenotype but not with a more advanced disease state, which is in agreement with Karim et al [21] who found that, while cytoplasmic Wnt1 expression increased with increasing tumour grade, it was not a statistically significant difference. Indeed, a more recent study [22] reported that Wnt1 is only involved in the initial phases of breast cancer development, where it supports differentiation and apoptosis of the tumour cells.…”

Section: Discussionmentioning

confidence: 99%

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Expression Profile of Wnt/β-Catenin Signalling Molecules and the Wnt Antagonist Secreted Frizzled-Related Protein 4 in Apoptosis in Breast Cancer Tissue Micro-Arrays

et al. 2014

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Wnt proteins are often up-regulated in cancer. The secreted frizzled-related proteins (sFRPs) can abrogate Wnt signalling and are involved in apoptosis. We investigated the expression of Wnt1, -Catenin, and an antagonist, sFRP4, as well as apoptosis in breast cancer using tissue micro-arrays (TMAs) comprising 191 tissue cores. Results demonstrated stronger staining intensity for Wnt1 in tumour versus non-tumour samples (p<0.05). Epithelial sFRP4 did not differ between invasive and non-invasive tissue; however, there was increased sFRP4 expression in the blood vessels and lymphocyte cells of tumour compared to non-tumour tissue. These data suggest Wnt involvement in determining the breast cancer phenotype and highlight a potential new role for sFRP4 as a diagnostic/prognostic marker.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Expression Profile of Wnt/β-Catenin Signalling Molecules and the Wnt Antagonist Secreted Frizzled-Related Protein 4 in Apoptosis in Breast Cancer Tissue Micro-Arrays

et al. 2014

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“…The study was approved by the institutional ethical committee of the Medical School of the University of Athens. Informed consent was signed by all patients under study in order to use for research purposes their biological samples and clinical data [27].…”

Section: Methodsmentioning

confidence: 99%

High Farnesoid X Receptor (FXR) expression is a strong and independent prognosticator in invasive breast carcinoma

Giaginis¹,

Karandrea²,

Alexandrou³

et al. 2017

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Farnesoid X Receptor (FXR), a nuclear receptor superfamily member, is related with bile acids, glucose and lipids metabolism and recently with cancer. In the present study the clinical significance of FXR expression in invasive breast carcinoma was evaluated. FXR protein expression was assessed immunohistochemically on paraffin-embedded breast cancer tissues obtained from 115 breast cancer patients and was statistically analyzed with clinicopathological parameters, estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) expression, as well as with tumor cells' proliferative capacity and overall and disease-free patients' survival. FXR positivity was noted in 91 (79.1%) and high FXR expression in 51 (44.3%) out of 115 invasive breast carcinoma cases. High FXR expression was significantly associated with smaller tumor size (p=0.0318) and increased tumor cells' proliferative rate (p=0.0375). Invasive breast carcinoma patients presenting high FXR expression showed significantly longer overall and disease-free survival times compared to those with low FXR expression (log-rank test, p=0.0052 and p=0.0058). In multivariate analysis, FXR expression was identified as independent prognostic factor of overall and disease-free patients' survival (Cox-regression analysis, p=0.0023 and p=0.0049, respectively). The present data support evidence that FXR may be implicated at the earlier stage of breast malignant disease progression, being a strong and independent prognosticator of favorable overall and disease-free survival in invasive breast carcinoma.

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“…At the time of the last follow-up, 25 (20.7%) patients had died from disease, 15 (12.4%) were alive with disease and 81 (66.9%) were alive and disease-free. All patients received conventional postoperative treatment depending on the extent of the disease, including adjuvant chemotherapy, radiation therapy and anti-estrogen therapy, when indicated, according to the consensus recommendations at the time [35]. [19,36].…”

Section: Patientsmentioning

confidence: 99%

Elevated Hu-Antigen Receptor (HuR) Expression is Associated with Tumor Aggressiveness and Poor Prognosis but not with COX-2 Expression in Invasive Breast Carcinoma Patients

Giaginis

Sampani

Kotta-Loizou

et al. 2017

Pathol. Oncol. Res.

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Hu-antigen R (HuR), a RNA-binding protein, is considered to play a crucial role in tumor development and progression by stabilizing or regulating a group of cellular mRNAs of cancer-related genes, such as cyclooxygenase-2 (COX-2). The present study aimed to evaluate the clinical significance of HuR and COX-2 expression in invasive breast carcinoma. HuR and COX-2 protein expression was assessed immunohistochemically on paraffin-embedded breast cancer tissue sections obtained from 121 patients and was statistically analyzed with clinicopathological parameters, estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), as well as with tumor cells' proliferative capacity and overall and disease-free patients' survival. High HuR expression was positively associated with larger tumor size and advanced disease stage (p = 0.0234 and p = 0.0361, respectively), being more frequently observed in ER negative cases (p = 0.0208). High COX-2 expression was negatively associated with histological (p < 0.0001) and nuclear (p = 0.0033) grade and tumor cells' proliferative rate (p = 0.0015), being more frequently observed in luminal-A compared to other molecular subtypes (p = 0.0221). High HuR expression was associated with poor overall and disease-free patients' survival at both univariate (log-rank test, p = 0.0092 and p = 0.0004, respectively) and multivariate (Cox-regression analysis, p = 0.0223 and p = 0.0004, respectively) level. On the other hand, high COX-2 expression was associated with favorable overall and disease-free patients' survival merely at univariate level (log-rank test, p = 0.0389 and p = 0.0154, respectively). HuR expression was not associated with COX-2 expression (Spearman R = 0.1489, p = 0.1032). The present data support evidence that HuR is associated with tumor aggressiveness and poor prognosis in breast carcinoma, reinforcing its potential as promising therapeutic target in this type of neoplasia.

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An immunohistochemical evaluation of the proteins Wnt1 and glycogen synthase kinase (GSK)‐3β in invasive breast carcinomas

Cited by 12 publications

References 30 publications

Expression Profile of Wnt/β-Catenin Signalling Molecules and the Wnt Antagonist Secreted Frizzled-Related Protein 4 in Apoptosis in Breast Cancer Tissue Micro-Arrays

Expression Profile of Wnt/β-Catenin Signalling Molecules and the Wnt Antagonist Secreted Frizzled-Related Protein 4 in Apoptosis in Breast Cancer Tissue Micro-Arrays

High Farnesoid X Receptor (FXR) expression is a strong and independent prognosticator in invasive breast carcinoma

Elevated Hu-Antigen Receptor (HuR) Expression is Associated with Tumor Aggressiveness and Poor Prognosis but not with COX-2 Expression in Invasive Breast Carcinoma Patients

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