An Immunosensor Using Electroactive COF as Signal Probe for Electrochemical Detection of Carcinoembryonic Antigen

Liang, Huihui; Luo, Ying; Li, Yanyan; Song, Yonghai; Wang, Linyu

doi:10.1021/acs.analchem.1c05426

Cited by 97 publications

(34 citation statements)

References 54 publications

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“…Common tumor markers include carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 24-2 [ 16 , 17 ]. CEA is a structural protein and one of the components of cell membranes; it can be detected in the serum of patients with CRC [ 18 ]. Elevated CEA is common in gastrointestinal tumors, urinary tract tumors, lung cancer, breast cancer, and other diseases.…”

Section: Introductionmentioning

confidence: 99%

Expression Levels and Clinical Significance of Serum miR-497, CEA, CA24-2, and HBsAg in Patients with Colorectal Cancer

Liu

Chen

2022

BioMed Research International

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The objective of the current study was to look at the levels of blood micro ribonucleic acid- (miR-) 497, carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 24-2, and hepatitis B surface antigen (HBsAg) in patients with colorectal cancer (CRC), as well as the clinical importance of these markers in CRC patients. The serum levels of miR-497, CEA, CA24-2, and HBsAg were compared between 60 patients with CRC (observation group) and another 60 patients with colorectal polyps (control group). The 4 indicators in patients with lymph node metastasis and liver metastasis were compared. The diagnostic effects of 4 detection methods and the combined detection were analyzed, and the influence of 4 indicators on the 5-year cumulative survival rate of patients was discussed. The results showed that the serum levels of miR-497 and HBsAg were lower, and the levels of CEA and CA24-2 were higher in the observation group ( P < 0.05 ). The combined detection had the best diagnostic effect, and CEA alone had the best prediction effect. The serum level of miR-497 was significantly lower in patients with lymphatic metastasis, with the significantly higher levels of CEA and CA24-2 ( P < 0.05 ). The HBsAg level of patients with liver metastases was greatly lower than that of patients without liver metastases ( P < 0.05 ). The 5-year cumulative survival rate of patients with high levels of CEA and CA24-2 was significantly lower than that of patients with low level of CEA. The 5-year cumulative survival rate was lower in patients with low level of HBsAg, but the difference was small. The 5-year cumulative survival rate of patients with elevated serum miR-497 was observably lower. In conclusion, combined detection could diagnose CRC more accurately. Serum miR-497, CEA, and CA24-2 were important in the diagnosis of lymph node metastasis of CRC. HBsAg did a better job of predicting liver metastases in CRC patients. High level of CEA significantly reduced the cumulative survival rate of CRC patients and could predict the long-term survival rate of patients. Serum levels of miR-497, CEA, CA24-2, and HBsAg played a positive role in the diagnosis and evaluation of CRC and could identify lymph node and liver metastases, having a high clinical guidance value.

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Section: Introductionmentioning

confidence: 99%

Expression Levels and Clinical Significance of Serum miR-497, CEA, CA24-2, and HBsAg in Patients with Colorectal Cancer

Liu

Chen

2022

BioMed Research International

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“…According to a previous report [ 36 ], the redox mechanism is speculated to be that the nitrogroup contained in the structure of furazolidone is reduced under the synergistic catalysis of Pt and rGO. With the increase in the scanning rate, the peak current density of furazolidone increased ( Figure 3 c), and a good linear relationship was presented ( Figure 3 d), indicating that the reaction process was a typical surface control process [ 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

A Paper-Based Electrochemical Sensor Based on PtNP/COFTFPB−DHzDS@rGO for Sensitive Detection of Furazolidone

et al. 2022

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Herein, a paper-based electrochemical sensor based on PtNP/COFTFPB−DHzDS@rGO was developed for the sensitive detection of furazolidone. A cluster-like covalent organic framework (COFTFPB−DHzDS) was successfully grown on the surface of amino-functional reduced graphene oxide (rGO-NH2) to avoid serious self-aggregation, which was further loaded with platinum nanoparticles (PtNPs) with high catalytic activity as nanozyme to obtain PtNP/COFTFPB−DHzDS@rGO nanocomposites. The morphology of PtNP/COFTFPB−DHzDS@rGO nanocomposites was characterized, and the results showed that the smooth rGO surface became extremely rough after the modification of COFTFPB−DHzDS. Meanwhile, ultra-small PtNPs with sizes of around 1 nm were precisely anchored on COFTFPB−DHzDS to maintain their excellent catalytic activity. The conventional electrodes were used to detect furazolidone and showed a detection limit as low as 5 nM and a linear range from 15 nM to 110 μM. In contrast, the detection limit for the paper-based electrode was 0.23 μM, and the linear range was 0.69–110 μM. The results showed that the paper-based electrode can be used to detect furazolidone. This sensor is a potential candidate for the detection of furazolidone residue in human serum and fish samples.

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“…redox reaction. [73] These COF-based electrochemical immunosensors show wide detection range, low detection limit, and practicality, indicating outstanding application prospects for determining biomarkers in clinical analysis.…”

Section: Antigen/antibody@cofmentioning

confidence: 99%

“…When CEA is captured by Ab1@COF Tab‐Dva , two units of electrons are transferred between COF TFPB‐Thi (reduction) and COF TFPB‐Thi (oxidation); therefore, the amount of CEA can be quantified based on the changes in the peak signal intensity of the COF TFPB‐Thi redox reaction. [ 73 ] These COF‐based electrochemical immunosensors show wide detection range, low detection limit, and practicality, indicating outstanding application prospects for determining biomarkers in clinical analysis.…”

Section: Biomedical Applications Of Biomacromolecule@cofmentioning

confidence: 99%

Covalent Organic Frameworks as a Biomacromolecule Immobilization Platform for Biomedical and Related Applications

Wang

Lewis

Wang

et al. 2022

Advanced Therapeutics

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With the development of biotherapeutic field, biomacromolecules with definite curative and small side effects have gradually become a hot spot in the research and development of new drugs. However, the use of biomacromolecules as drug candidates pose some technical difficulties related to their medicinal properties, mainly drug stability and in vivo transport and delivery. Currently, diverse nanodrug delivery systems have been used to deliver biomacromolecules because they can maintain stability, significantly increase bioavailability, and improve targeted distribution of biomacromolecules, thereby improving efficacy and reducing toxicity. In recent years, covalent organic frameworks (COFs) have attracted increasing interest as stable, efficient, and reusable biomacromolecule immobilization carriers. COFs exhibit good stability and biocompatibility with no biological toxicity, making them outstanding host materials for immobilizing biomacromolecules. However, the synthesis of COFs requires severe experimental conditions; the commonly used strategies for immobilizing biomacromolecules in metal-organic frameworks (MOFs), such as biomimetic mineralization, one-pot synthesis, and coprecipitation, are likely not applicable to COFs. Herein, three strategies for immobilizing biomacromolecules in COFs and the multifunctional biomedical applications of the biomacromolecule@COF composite systems are reviewed. Next, the superior properties and cutting-edge applications of MOFs are mentioned and then the advantages of COF-MOF hybrid materials are highlighted.

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An Immunosensor Using Electroactive COF as Signal Probe for Electrochemical Detection of Carcinoembryonic Antigen

Cited by 97 publications

References 54 publications

Expression Levels and Clinical Significance of Serum miR-497, CEA, CA24-2, and HBsAg in Patients with Colorectal Cancer

Expression Levels and Clinical Significance of Serum miR-497, CEA, CA24-2, and HBsAg in Patients with Colorectal Cancer

A Paper-Based Electrochemical Sensor Based on PtNP/COFTFPB−DHzDS@rGO for Sensitive Detection of Furazolidone

Covalent Organic Frameworks as a Biomacromolecule Immobilization Platform for Biomedical and Related Applications

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