An impedance-based integrated biosensor for suspended DNA characterization

Ma, Hanbin; Wallbank, Richard W. R.; Chaji, Reza; Li, Jiahao; Suzuki, Yuji; Jiggins, Chris D.; Nathan, Arokia

doi:10.1038/srep02730

Cited by 55 publications

(41 citation statements)

References 34 publications

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“…Measuring DNA or protein using fluorescent labels requires expensive and precise equipment and has unexpected interference with detection systems [1]. On the other hand, electrical sensors enable the label-free detection by measuring electrical signals instead of fluorescence brightness.…”

Section: Introductionmentioning

confidence: 99%

“…It is a very promising bio-sensing method because the limits of detection are very low and the time-to-results are relatively fast [2]. Recently, it is called electrochemical impedance spectroscopy (EIS) because it primarily measures the impedance change depending on the concentration of DNA or the amount of protein captured in the antibody [1,4].…”

Section: Introductionmentioning

confidence: 99%

“…These electrodes are expected to have different properties each time they are made, but research on the variations of the characteristics is hard to find. Instead, calibration is performed taking into account changes in electrode properties before use [1,6].…”

Section: Introductionmentioning

confidence: 99%

“…For example, to determine the concentration of prostate-specific antigen associated with prostate cancer, three gold electrodes were prepared by photolithography on a silicon substrate [3]. There are also cases where an electrode array is made using CMOS technology and used for DNA detection [1]. On the other hand, in order to observe the motion of the cell, an electrode is formed on glass or printed circuit board (PCB) substrate [5,7].…”

Section: Introductionmentioning

confidence: 99%

“…For measurement of impedance as in the reference [1], saline solution was distributed on electrodes and measured by sinusoidal stimulation using transimpedance amplifier. Assuming that the electrode width is main cause of the impedance fluctuation, it is expected that the fluctuation becomes smaller when the electrode is arranged in multiple layers.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Reducing Impedance Fluctuation by Electrode Structure for Electrochemical Impedance Spectroscopy

Park¹,

Park²,

Kim³

et al. 2017

Advanced Science and Technology Letters

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Abstract. In this paper, we investigated the electrode structure of the electrical bio-sensor to reduce impedance measurement fluctuation. Five pairs of the same electrodes were formed on the PCB substrate, and a fluidic channel was constructed with a double-sided tape and polycarbonate cover so that all the electrodes had the same reagent distribution. In order to find an electrode structure with little change in impedance, the structure of the electrode pairs arranged on the PCB substrate was divided into five types, one to five layers. The electrodes arranged on the PCB substrate are made the same size and the spacing between the electrode pairs is the same. As a result of an experiment in which physiological saline was injected into an electrode chip made of five electrode structures, it was confirmed that the change in the impedance between the electrodes was small as the number of electrode layers was increased.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reducing Impedance Fluctuation by Electrode Structure for Electrochemical Impedance Spectroscopy

Park¹,

Park²,

Kim³

et al. 2017

Advanced Science and Technology Letters

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Ultrasensitive Detection of Mitochondrial DNA Mutation by Graphene Oxide/DNA Hydrogel Electrode

Sun

Peng

et al. 2014

Adv Funct Materials

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6905wileyonlinelibrary.com of DNA. The bionic structure benefi ts the adsorption and hybridization of DNA. [ 3 ] The intermediate conductivity of electrode is sensitive to the change of conductivity introduced by trace DNA on the surface of electrode. On the contrary, this weak change is unobvious for the electrode with too high or low conductivity. As is well known, the conductivity of semiconductor silicon can be increased by adding certain impurities in minute quantities. Graphene oxide (GO) is a semiconductor and its conductivity can be improved by doping negatively charged molecules. [ 4 ] Therefore, the conductivity of hydrogel containing GO, water and natural biomolecules would be very sensitive to negatively charged molecules, such as DNA, and this hydrogel could be developed as novel DNA biosensor with high sensitivity.Fish sperm DNA is a natural DNA extracted from fi sh sperm and could form hydrogel with GO. [ 5 ] The GO/DNA hydrogel contains three components: GO, fi sh sperm DNA and water. Every component plays an important role in improving the sensitivity of DNA detection. GO is a semiconductor with intermediate conductivity compared with its reduced form (RGO) and most polymers, [ 6 ] which might produce a large conductivity change when trace DNA molecule was introduced. The hydrogel contains a large amount of fi sh sperm DNA molecules that could provide much higher biocompatibility than that of other chemical cross−linkers, which is advantageous for DNA hybridization in this bionic structure. The hydrogel also contains rich water that is similar to the natural environment for DNA hybridization, which is also benefi cial to improving the hybridization effi ciency of DNA. In addition, the hydrogel has a large specifi c surface and high diffusivity of small molecules [ 7 ] besides the biocompatibility. Therefore, it is a viable candidate for sensory and actuation applications. The sensitivity of biosensor is infl uenced by the conductivity of hydrogel which depends upon its thickness and component. [ 8 ] Conventionally, the hydrogel is simply coated onto the surface of classical electrodes, which makes the shape and thickness of hydrogel irregular and uncontrollable, respectively. One possible route is to form the hydrogel directly in a tube so that the shape would be fi xed and the thickness could be controlled as needed. The conductivity of hydrogel could be tuned by changing its component and thickness.Herein, we reported a hydrogel electrode prepared from GO and fi sh sperm DNA ( Scheme 1 ). The mixed solution of GO and Ultrasensitive detection of nucleic acid has attracted considerable attention recently in academic research and clinic diagnostics. Current approaches for DNA analysis involve complicated or expensive processes for labeling and often yield a high detection limit. In this study, a hydrogel electrode prepared from graphene oxide and fi sh sperm DNA is used for label−free mitochondrial DNA detection by impedimetric approach. The hydrogel has a bionic structure containing rich water ...

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Electrochemical Properties of Highly Sensitive and Selective CuO Nanostructures Based Neurotransmitter Dopamine Sensor

et al. 2017

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In this article, electrochemical properties of CuO nanostructures based dopamine (DA) sensor was investigated. The morphology, structure, optical, and compositional properties of the CuO nanostructures were characterized by using SEM, XRD, UV‐Vis, and XPS techniques. The electrochemical properties were investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The CV results indicate that biosensors based on CuO nanostructures exhibit a high selectivity and sensitivity of 0.1975 μA μM–1 toward DA and effectively avoids the interference of ascorbic acid (AA) and uric acid (UA). The obtained EIS spectra for CuO sensors were analysed using an electrical equivalent circuit to understand the bulk and surface response via the capacitive and resistive parameters. The EIS measurement also leads to the direct determination of parameters like series resistance and ion diffusion phenomena at electrode‐electrolyte interface. The experimental CV and EIS results along with their analysis will have a significant impact on understanding the mechanism of high sensitivity and selectivity performance of CuO based sensors. This study may also lay the basis for efficient characterization of biosensors by coupling both the CV and EIS characterization techniques.

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An impedance-based integrated biosensor for suspended DNA characterization

Cited by 55 publications

References 34 publications

Reducing Impedance Fluctuation by Electrode Structure for Electrochemical Impedance Spectroscopy

Reducing Impedance Fluctuation by Electrode Structure for Electrochemical Impedance Spectroscopy

Ultrasensitive Detection of Mitochondrial DNA Mutation by Graphene Oxide/DNA Hydrogel Electrode

Electrochemical Properties of Highly Sensitive and Selective CuO Nanostructures Based Neurotransmitter Dopamine Sensor

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