An improved capillary model for describing the microstructure characteristics, fluid hydrodynamics and breakthrough performance of proteins in cryogel beds

Yun, Junxian; Jespersen, Gry Ravn; Kirsebom, Harald; Gustavsson, Per-Erik; Mattìasson, Bo; Galaev, Igor Yu.

doi:10.1016/j.chroma.2011.06.056

Cited by 32 publications

(35 citation statements)

References 86 publications

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“…where N g is the total number of capillary groups with the same diameter, n i the number of capillaries in group i, Q i the flow rate [80] 4 /(128 )…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

“…The concentration at the out let of the cryogel bed was obtained by averaging the concentrations in capillaries based on their flow rates and written as [80] …”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

“…The model was solved numerically by the finite difference method applied to poly(hydroxyethyl methacrylate), PHEMA, cryogels prepared by radical cryo-copolymerisation. The authors analysed the axial dispersion behaviour, the pressure drop vs. flow rate performance, the non-adsorption breakthrough curves of lysozyme, bovine serum albumin, BSA, and concanavalin A, at various flow velocities in the cryogel beds [80]. The model developed was based on the structural characteristics (diameter, length, porosity, and permeability) and the analysis of the protein breakthrough curves gave detailed information on the textural and adsorption properties of the cryogels.…”

mentioning

confidence: 99%

“…3). However, the direct calculations of the PSD with quantitative analysis of SEM images were not carried out in this paper [80]. Additionally, Jurga et al [112] prepared bioactive scaffolds using cryogelation of dextran or gelatine linked to laminin.…”

mentioning

confidence: 99%

“…One of the most advanced structural characterisation of cryogels was performed by Yun et al [80]. They developed a capillary-based model to characterise the structure of monolithic cryogels applying such key parameters as the PSD, f(d i ), determined from Eq.…”

mentioning

confidence: 99%

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Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

286

205

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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“…where N g is the total number of capillary groups with the same diameter, n i the number of capillaries in group i, Q i the flow rate [80] 4 /(128 )…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

“…The concentration at the out let of the cryogel bed was obtained by averaging the concentrations in capillaries based on their flow rates and written as [80] …”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

286

205

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Cryogel‐based co‐culture of Lactobacillus paracasei and Lactobacillus buchneri towards phenyllactic acid bioproduction: fundamental hydrodynamics and biotransformation characteristics

Lou

Hou

Chen

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND: Phenyllactic acid is an important monomer for bioplastics, food preservative, feed additive and pharmaceutical agent. However, bioproduction of phenyllactic acid suffers drawbacks regarding the high cost of substrates, poor efficiency and yield of fermentation or conversion, and therefore new bioproduction strategies need to be investigated. RESULTS: In this work, bioproduction of phenyllactic acid was developed by employing semi-hydrophobic cryogels as cell entrapment matrices to co-culture Lactobacillus paracasei and L. buchneri strains, and the obtained cell-loaded cryogels were used as biocatalysts in the biotransformation from phenylalanine. Flow characteristics and permeabilities of fermentation broths through cryogels, co-culture performance and biotransformation characteristics were studied. Permeabilities of cell-free fermentation broths through the cryogel bed were correlated as a function of concentrations of glucose and metabolites of organic acids. The flow rate versus pressure drop relationships for these bio-based broths through cryogels were predicted successfully with a capillary-based model. The cell growth was enhanced by cryogels and the maximum cell production of 40.6 g L −1 was achieved with cryogels. The maximum yield from phenylalanine of 1.0 mg mL −1 to phenyllactic acid was 39.6%, which was higher than that of 31.4% by co-culture of L. casei and L. paracasei.CONCLUSION: Flow of fermentation broths through cryogels is crucial to develop a novel cryogel-based bioproduction strategy for phenyllactic acid and can be characterized by capillary-based modeling. Semi-hydrophobic cryogels could be an interesting class of immobilization matrices for high-cell-density co-culture towards the green strategy with these new biocatalysts in phenyllactic acid bioproduction and thus have potential applications in industries.

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Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)‐based anion‐exchange cryogel

Dong

Chen

Dai

et al. 2013

J of Separation Science

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Bovine milk whey contains several bioactive proteins such as α-lactalbumin, β-lactoglobulin, and immunoglobulin G (IgG). Chromatographic separation of these proteins has received much attention in the past few years. In this work, we provide a chromatographic method for the efficient isolation of IgG from bovine milk whey using a poly(2-hydroxyethyl methacrylate)-based anion-exchange cryogel. The monolithic cryogel was prepared by grafting 2-(dimethylamino) ethyl methacrylate onto the poly(2-hydroxyethyl methacrylate)-based cryogel matrix and then employed to separate IgG under various buffer pH and salt elution conditions. The results showed that the buffer pH and the salt concentration in the step elution have remarkable influences on the purity of IgG, while the IgG recovery depended mainly on the loading volume of whey for a given cryogel bed. High purity IgG (more than 95%) was obtained using the phosphate buffer with pH of 5.8 as the running buffer and the salt solution in as the elution liquid. With suitable loading volume of whey, the maximum IgG recovery of about 94% was observed. The present separation method is thus a potential choice for the isolation of high-purity IgG from bovine milk whey.

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An improved capillary model for describing the microstructure characteristics, fluid hydrodynamics and breakthrough performance of proteins in cryogel beds

Cited by 32 publications

References 86 publications

Cryogels: Morphological, structural and adsorption characterisation

Cryogels: Morphological, structural and adsorption characterisation

Cryogel‐based co‐culture of Lactobacillus paracasei and Lactobacillus buchneri towards phenyllactic acid bioproduction: fundamental hydrodynamics and biotransformation characteristics

Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)‐based anion‐exchange cryogel

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