An Improved Chemically Defined Basal Medium (Cmrl-1415) for Newly Explanted Mouse Embryo Cells

Healy, George M.; Parker, Raymond C.

doi:10.1083/jcb.30.3.531

Cited by 80 publications

(8 citation statements)

References 25 publications

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“…Single cell suspensions were obtained from the femurs of the fasted rats by forcing cold CMRL-1415 (Healey & Parker, 1966) from a 23 gauge needle through the marrow cavity. The resulting cell suspension was washed by centrifugation (700 g at 4°C for 15 min) and resuspended in 5 ml CMRL-1415.…”

Section: A T E R I a L S A N D M E T H O D S 1 Preparation Of Cellmentioning

confidence: 99%

Erythropoietin Sensitivity of Rat Bone Marrow Cells Separated by Velocity Sedimentation

et al. 1970

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A B S T R A C TRat bone marrow cells have been separated on the basis of their sedimentation at unit gravity. The cell population most responsive to erythropoietin in vitro was found to have a sedimentation velocity of about 6.6 mm/hr. In the process of becoming hemoglobin-synthesizing cells, it undergoes cell division and its sedimentation velocity decreases to 3.9 mm/hr and then to 2.1 mm/hr, the sedimentation velocity of mature red blood cells.

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Section: A T E R I a L S A N D M E T H O D S 1 Preparation Of Cellmentioning

confidence: 99%

Erythropoietin Sensitivity of Rat Bone Marrow Cells Separated by Velocity Sedimentation

et al. 1970

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“…Fibroblasts were grown in tissue culture media [4] and cryopreserved in liquid nitrogen as a 'bank' of cells as previously described [2], When needed, the cells were thawed, concentrated and resuspended [2] at a concentration o f 2.5 X 106 cells/ml for intravi treal injection.…”

Section: Cell Culturementioning

confidence: 99%

Further Observations on the Prevention of Experimental Proliferative Vitreo-Retinopathy by 5-Fluorouracil

Ophir¹

1991

Ophthalmic Res

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The rate of retinal detachment in fellow control eyes of rabbits following fibroblast administration is progressively lower with increased dose of 5-Fluorouracil in the test eyes. Thus, it seems mandatory to choose control eyes in other animals rather than the fellow eyes of the treated ones. Retinal detachment appeared in the second week or earlier after intravitreal fibroblast administration. However, in eyes treated intravitreally with 5-Fluorouracil, retinal detachment or pucker appeared later, even in the second month suggesting routine follow-up of at least 2 months in future studies.

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“…Recently, medium CMRL-1415 was developed for the growth of primary cultures of mouse embryonic tissue (10). An important change in this medium was the introduction of a stronger buffering system attributed, in part, to the use of certain free-base amino acids and to a fourfold increase in the concentration of dibasic sodium phosphate.…”

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confidence: 99%

Improved Chemically Defined Basal Medium (CMRL-1969) for Primary Monkey Kidney and Human Diploid Cells 1

Healy¹,

Teleki²,

Seefried³

et al. 1971

Applied Microbiology

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An improved tissue culture basal medium, CMRL-1969, supplemented with serum, has been evaluated by measuring the growth responses of primary cultures of trypsin-dispersed monkey kidney cells (PMKC) and of an established culture of a human diploid cell strain (HDCS). Medium H597, an early modification of medium 199 which has been used successfully in the preparation of poliomyelitis vaccine for 15 years, was used for comparison. In addition, parallel testing was done with Basal Medium Eagle (BME) widely used for the growth of HDCS. The improvements in basal medium CMRL-1969 are attributed to changes in amino acid concentrations, in vitamin composition, and, in particular, to enhanced buffering capacity. The latter has been achieved by the use of free-base amino acids and by increasing the dibasic sodium phosphate. The new medium has already been used to a dvantage for the production of polioviruses in PMKC where equivalent titers were obtained from cultures initiated with 70% of the number of cells required with earlier media. The population-doubling time was reduced in this system. Also, with small inocula of HDCS, the time required to obtain maximum cell yield was shorter with CMRL-1969 than with BME. Both media were supplemented with 10% calf serum. Maximum cell yields after repeated subcultivation in the new basal medium were greatly increased and the stability of the strain, as shown by chromosomal analysis, was not affected. Basal medium CMRL-1969 can be prepared easily in liquid or powdered form.

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An Improved Chemically Defined Basal Medium (Cmrl-1415) for Newly Explanted Mouse Embryo Cells

Cited by 80 publications

References 25 publications

Erythropoietin Sensitivity of Rat Bone Marrow Cells Separated by Velocity Sedimentation

Erythropoietin Sensitivity of Rat Bone Marrow Cells Separated by Velocity Sedimentation

Further Observations on the Prevention of Experimental Proliferative Vitreo-Retinopathy by 5-Fluorouracil

Improved Chemically Defined Basal Medium (CMRL-1969) for Primary Monkey Kidney and Human Diploid Cells 1

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