An improved chemiluminescence-based liposome immunoassay involving apoenzyme.

Haga, Makoto; Hoshino, Shoichiro; Okada, Hiroe; Hazemoto, Norio; Kato, Yuriko; Suzuki, Yasuo

doi:10.1248/cpb.38.252

Cited by 21 publications

(5 citation statements)

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“…All bound liposomes are lysed by addition of a detergent releasing the signal-generating marker (Figure 3). Different LIA methods (167)(168)(169)(170)(171)(172) were developed for analysis of various pharmaceutical compounds (Table 8).…”

Section: Liposome Immunoassaymentioning

confidence: 99%

Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Advances

Darwish

2006

Int. J Biomed Sci.

189

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Immunoassays are bioanalytical methods in which the quantitation of the analyte depends on the reaction of an antigen (analyte) and an antibody. Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries. The importance and widespread of immunoassay methods in pharmaceutical analysis are attributed to their inherent specificity, high-throughput, and high sensitivity for the analysis of wide range of analytes in biological samples. Recently, marked improvements were achieved in the field of immunoassay development for the purposes of pharmaceutical analysis. These improvements involved the preparation of the unique immunoanalytical reagents, analysis of new categories of compounds, methodology, and instrumentation. The basic methodologies and recent advances in immunoassay methods applied in different fields of pharmaceutical analysis have been reviewed.

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Section: Liposome Immunoassaymentioning

confidence: 99%

Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Advances

Darwish

2006

Int. J Biomed Sci.

189

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“…A possible strategy for performing liposome-based immunoassays consists of replacing the enzyme by specific probes encapsulated into the liposome cavity that can be detected by colorimetric, [10] fluorimetric, [11] chemiluminometric, [12] photothermic [13] or electrochemical methods. [14] The choice of the detection method is typically made on the basis of the equipment available in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

“…The herein reported results show how the proposed method is competitive in terms of the achievable sensitivity in respect to the currently available methods, with the great advantage of using a well-established, simple, fast responding, low cost detection technique as a laboratory pH-meter. Although different methods and strategies by using liposomes have been numerously reported for the bioassays on the basis of various signal-generation principles, [10][11][12][13][14] there is no report focusing on liposome-based pH readout in the bioassays until now. Moreover, the great advantage of the proposed method over existing ones relies in the reduction of the analysis costs.…”

Section: Introductionmentioning

confidence: 99%

LipHosomes: Reporters for Ligand/Anti‐Ligand Assays Based On pH Readout

et al. 2020

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A new class of liposomes (LipHosomes) is designed to induce a change of pH upon releasing their content. pH‐readout reports on the number of LipHosomes in the specimen. LipHosomes were prepared by entrapping NaOH or bicarbonate buffer in the intravesicular compartment. The liposomes suspension was purified from unentrapped compounds and brought to pH 7.0. The pH gradient between intra‐ and extra‐liposomal compartments is maintained because the phospholipidic membrane works as a semipermeable membrane thus preventing diffusion of ions across the membrane. The release of the liposomal content triggers a quantifiable variation of the pH of the medium. This feature has been harnessed in analytical assays based on ligand/anti‐ligand molecular recognition by exploiting the biotin‐streptavidin binding scheme. A pH difference of 0.2 units was observed upon the release of the payload from biotinylated LipHosomes bound to streptavidinated plates. The test showed an excellent sensitivity being able to reveal a concentration of bound LipHosomes in the sub‐pM range.

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“…Thereafter, enzyme-linked immunosorbent assays (ELISA) [10,11] and chemiluminescence immunoassay (CLIA) [12,13] were widely used in clinical test and many commercially available assay kits were developed. Besides, colorimetric immunoassay [14], time-resolved fluoroimmunoassay [15,16], and liposome immunoassay [17,18] have also been reported for CEA analysis. In recent years, the electrochemical immunosensor has been one of the attractive analytical tools used in CEA detection due to its simplicity and high sensitivity [19,20].…”

Section: Introductionmentioning

confidence: 99%

Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

Zhang

Zhao

et al. 2009

Sci. China Chem.

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A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in micro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H 2 O 2 )-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL 1. The detection limit of CEA was 0.61 ng mL 1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation. micro-plate magnetic chemiluminescence immunoassay, carcinoembryonic antigen, tumor marker

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An improved chemiluminescence-based liposome immunoassay involving apoenzyme.

Cited by 21 publications

References 0 publications

Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Advances

Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Advances

LipHosomes: Reporters for Ligand/Anti‐Ligand Assays Based On pH Readout

Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

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