An Improved Ferritin Assay for Canine Sera

Andrews, Gordon A.; Smith, Joseph; Gray, Mary Ann; Chavey, Patricia S.; Weeks, Brad R.

doi:10.1111/j.1939-165x.1992.tb00584.x

Cited by 31 publications

(27 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This attests to both the reproducibility of the assay and the stability of frozen ferritin. The within-assay variability and assay-to-assay variabilities were comparable to those observed with other monoclonal antibody-based ferritin assays [Weeks et al, 1988;Andrews et al, 1992Andrews et al, , 1994.…”

Section: Discussionsupporting

confidence: 77%

“…However, the finding that anti-equine ferritin antibodies cross react with rhinoceros ferritin, and the subsequent development of an ELISA test to measure serum ferritin in the rhinoceros [Smith et al, 1995] led us to investigate whether we could develop an assay to measure fur seal ferritin using murine monoclonal antibodies previously raised to canine ferritin [Andrews et al, 1992].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme‐linked immunosorbent assay to quantitate serum ferritin in the northern fur seal (Callorhinus ursinus)

et al. 2004

Self Cite

View full text Add to dashboard Cite

Serum ferritin concentration correlates with tissue iron stores in humans, horses, calves, dogs, cats, and pigs. Serum ferritin is considered the best serum analyte to predict total body iron stores in these species, and is more reliable than serum iron or total iron-binding capacity, both of which may be affected by disorders unrelated to iron adequacy or excess (including hypoproteinemia, chronic infection, hemolytic anemia, hypothyroidism, renal disease, and drug administration). Iron overload has been documented to result in hemochromatosis in captive northern fur seals (Callorhinus ursinus); therefore, we developed an enzyme-linked immunosorbent assay (ELISA) to measure serum ferritin in this species. The assay uses two murine anti-canine ferritin monoclonal antibodies in a sandwich arrangement that was originally used in an ELISA to measure serum ferritin in dogs. Ferritin isolated from fur seal liver was used as a standard. Ferritin standards were linear from 0 to 50 ng/ml. Recovery of purified ferritin from fur seal serum varied from 89% to 99%. The within-assay variability was 6%, and the assay-to-assay variability for two different samples was 10% and 16%.

show abstract

Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Enzyme‐linked immunosorbent assay to quantitate serum ferritin in the northern fur seal (Callorhinus ursinus)

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…As técnicas para a determinação da ferritina sérica são reações dirigidas por anticorpos, como radioimunoensaio, ELISA, imunoturbidimetria e quimioluminescência, de alta sensibilidade. Os reagentes comerciais de ELISA para a dosagem de ferritina sérica em cães já foram desenvolvidos nos Estados Unidos (WEEKS et al, 1988;ANDREWS et al, 1992) e no Japão (WATANABE et al, 2000), no entanto, não são comercializados, devido ao custo elevado para a produção inviabilizando sua exportação.…”

Section: Introductionunclassified

Parâmetros utilizados na avaliação do metabolismo do ferro em cães

et al. 2011

View full text Add to dashboard Cite

RESUMO A avaliação do metabolismo de ferro associado aos resultados hematológicos constitui uma ferramenta importante para o diagnóstico, controle e diferenciação de estados anêmicos. O presente trabalho objetivou determinar ABSTRACT The determination of iron metabolism rates associated with hematological findings is an important tool to diagnosis, control and differentiation of anemic states. This study has as objective to determine the iron parameters (serum iron, unsaturated iron binding-capacity, total iron bindingcapacity, transferrin saturation rate (IST), transferrin and ferritin) in healthy dogs. It was selected 120 dogs, shared in four different groups of sex and age (30 young males and 30 adult males, 30 young females and 30 adult females

show abstract

“…The values indicate the means measured in triplicate, and the error bars show standard deviation. concentrations determined previously in normal dogs by Andrews [1] and Watanabe [14] were 252 ng/ml (n=61, 80-800 ng/ml) and 479 ng/ml (n=51, 298-959 ng/ml), respectively. The values reported in this study are obviously much higher.…”

mentioning

confidence: 99%

“…From past reports, detection of serum ferritin in dogs has been accomplished by sandwich enzyme-linked immunosorbent assays (ELISAs) using a mouse anti-canine ferritin monoclonal antibody, and purified canine liver ferritin as the standard [1]. However, it has been reported that in canine serum an autoantibody exists that binds to ferritin.…”

mentioning

confidence: 99%

Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

Chikazawa

Hori

Hoshi

et al. 2013

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.

show abstract

An Improved Ferritin Assay for Canine Sera

Cited by 31 publications

References 9 publications

Enzyme‐linked immunosorbent assay to quantitate serum ferritin in the northern fur seal (Callorhinus ursinus)

Enzyme‐linked immunosorbent assay to quantitate serum ferritin in the northern fur seal (Callorhinus ursinus)

Parâmetros utilizados na avaliação do metabolismo do ferro em cães

Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

Contact Info

Product

Resources

About