An Improved Internal Surface Reversed Phase

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

“…An additional weak-cation exchanger functionality was demonstrated for this support due to the free carboxyl group of the terminal phenylalanine [24]. A second generation of GFF support (GFF II) was developed by Perry et al [25] to improve performances in terms of efficiency, retentivity and reproducibility. ISRP supports allowed to withstand several hundred plasma or serum injections (total volume of 6-7 ml) without losing performance [26,27].…”

Determination of phenobarbital in plasma by micellar liquid chromatography

“…The HPLC procedures reported for determining phenobarbital require sample pre-treatments such as protein precipitation (Perry et al, 1992), solvent extraction (Ohmacht et al, 1991;Kuroda et al, 1996), solidphase extraction (Kuono et al, 1993;Moriyama et al, 1994), dialysis (Johansen et al, 1995), or the use of column-switching techniques (Lubda et al, 1996;Kouno et al, 1997). Special chromatographic columns which allow the direct injection of the physiological samples have also been proposed (Perry et al, 1992;Haginaka and Wakai, 1992;Nimura et al, 1995).…”

“…Special chromatographic columns which allow the direct injection of the physiological samples have also been proposed (Perry et al, 1992;Haginaka and Wakai, 1992;Nimura et al, 1995).…”