2004
DOI: 10.1177/1087057104269496
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An Improved Method for Checking HTS/uHTS Liquid-Handling Systems

Abstract: An efficient method is presented to determine precision and accuracy of multichannel liquid-handling systems under conditions near to application. The method consists of gravimetrical determination of accuracy and optical determination of precision based on the dilution of absorbing and fluorescent dye solutions in microplates. Mean delivery volume per well can be determined with precision better than a 0.04% coefficient of variation (CV). Optical signal precision, CV(S), is improved by multiwavelength measure… Show more

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Cited by 34 publications
(39 citation statements)
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“…The evaluation method applied has been described elsewhere. 17 In short, the channel-to-channel coefficient of variation of the sample volumes delivered to a microplate, CV(V), is determined. The sample volume to be dispensed is set to 2 µL.…”
Section: Evaluation Of a Liquid-handling Devicementioning
confidence: 99%
“…The evaluation method applied has been described elsewhere. 17 In short, the channel-to-channel coefficient of variation of the sample volumes delivered to a microplate, CV(V), is determined. The sample volume to be dispensed is set to 2 µL.…”
Section: Evaluation Of a Liquid-handling Devicementioning
confidence: 99%
“…Because a modern LHS is usually equipped with capacity of liquid level detection, liquid volume can be measured by calculation based on microwell plate dimensions and liquid level in mircowell. However, gravimetric measurements have been used extensively to calibrate the liquid dispensers in LHS (Bergsdorf et al 2006;Rhode et al 2004). It utilizes the mass and the density of solution to determine the volume.…”
Section: %Ih = 100 × !J Kld7mentioning
confidence: 99%
“…For fluorophore-and absorbancebased methods, it is straightforward to use 80% to 100% DMSO solutions, 5,7 while measuring nanoliter volumes in more than 80% DMSO with ratiometric photometry presents technical challenges. 2,4,6 In fact, validated commercial ratiometric photometry solutions above 75% DMSO have previously not been available. 6 For high-throughput applications, dual-dye photometric methods are superior.…”
Section: Introductionmentioning
confidence: 99%