An improved method for genomic DNA extraction from strawberry leaves

Nunes, Claudinéia Ferreira; Ferreira, Juliano Lino; Fernandes, Mônique Carolina Nunes; Breves, Sâmera de Souza; Generoso, Andressa Leal; Soares, Bárbara Dantas Fontes; Dias, Mário Sérgio Carvalho; Pasqual, Moacir; Borém, Aluízio; Cançado, Geraldo Magela de Almeida

doi:10.1590/s0103-84782011000800014

Cited by 35 publications

(31 citation statements)

References 8 publications

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“…The samples were transported to the molecular biology laboratory at UFLA and stored at -80 ºC in ultra-low freezer. Leaf samples were lyophilized until complete dryness and subsequently grinded, followed by genomic DNA extraction using the method described by Nunes et al (2011).…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

“…Among the molecular markers available, microsatellites or SSRs (simple sequence repeats) and ISSRs (inter simple sequence repeats) are widely used to study the genetic diversity and population structure of many plant species. SSR markers are codominant and multiallelic, whereas ISSR markers are dominant and multilocus (Borém andCaixeta 2009, Faleiro 2007). The combination of two molecular markers can provide a more accurate inference regarding the genetic and genomic composition of a germplasm collection.…”

Section: Introductionmentioning

confidence: 99%

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Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Santos

Ferreira

Setotaw

et al. 2016

An. Acad. Bras. Ciênc.

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Jatropha is a potential oilseed crop, which requires mitigating factors such as the low genetic variability of the species. The solution runs through the research of Brazilian germplasm. Attention should be given to the germplasm of jatropha the north of Minas Gerais, because this is the oldest national collection and because this region may be a regions of jatropha diversity due to selection pressure arising from environmental adversities. Therefore, the objective of this study was to investigate the genetic diversity of 48 accessions of collection from Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), using SSR and ISSR markers. The results showed low genetic diversity, but some individuals stood out as J. mollissima (48), J. podagrica (47), Mexican accessions (42, 43, 44 and 45) and some national accessions (28, 29, 41 and 46). Therefore, aiming to increase the genetic variability and improve the effectiveness of jatropha breeding programs, it is suggested to explore such as parental accessions to generate commercial hybrids. This fact implies the possibility to support future production of jatropha, since this culture may be an important source of income, especially for small farmers living in semiarid regions of Brazil.

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Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Santos

Ferreira

Setotaw

et al. 2016

An. Acad. Bras. Ciênc.

Self Cite

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“…For conservation management to be performed using molecular techniques, a sufficient concentration of quality DNA is required for amplification reactions, digestions, and cloning (Nunes et al, 2011), as well as for further characterization and genetic diversity analyses. These studies typically involve numerous researchers and samples, necessitating an inexpensive and rapid method for DNA extraction.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

et al. 2014

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ABSTRACT. Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpinia ferrea are rare, it is important to develop and/or adapt a DNA extraction protocol that produces quality DNA samples to enable the design of strategies for the conservation of this threatened species. This study aimed to compare five methods for DNA extraction and to determine the most efficient protocol for C. ferrea. Sufficient genomic DNA was obtained from the leaves of C. ferrea using all the tested protocols to perform techniques DNA extraction from Caesalpinia ferrea involving molecular markers. Two protocols based on the detergent cetyl trimethyl ammonium bromide, as well as a commercial kit, yielded high concentrations of pure DNA. However, when polymerase chain reaction amplifications were performed, DNA was only successfully amplified from extractions performed with the commercial kit, which produced sufficient genomic DNA of good quality from the leaves of C. ferrea to perform techniques involving molecular markers.

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“…The leaves were transported to the laboratory and stored in a deep freezer at -80°C until DNA extraction. Genomic DNA was extracted according to the method developed by Nunes et al (2011). To verify the quality of the DNA, electrophoresis of sample DNA was performed on a 0.7% agarose gel with the addition of ethidium bromide (0.3 mg/mL), and the results were visualized under UV light using a Vetec photo documenter.…”

Section: Dna Extractionmentioning

confidence: 99%

Molecular characterization and population structure study of cambuci: strategy for conservation and genetic improvement

et al. 2016

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ABSTRACT. Cambuci (Campomanesia phaea) belongs to the Myrtaceae family and is native to the Atlantic Forest of Brazil. It has ecological and social appeal but is exposed to problems associated with environmental degradation and expansion of agricultural activities in the region. Comprehensive studies on this species are rare, making its conservation and genetic improvement difficult. Thus, it is important to develop research activities to understand the current situation of the species as well as to make recommendations for its conservation and use. This study was performed to characterize the cambuci accessions found in the germplasm bank of Coordenadoria de Assistência Técnica Integral using inter-simple sequence repeat markers, with the goal of understanding the plant's population structure. The results showed the existence of some level of genetic diversity among the cambuci accessions that could be exploited for the genetic improvement of the species. Principal coordinate analysis and discriminant analysis clustered the 80 accessions into three groups, whereas Bayesian modelbased clustering analysis clustered them into two groups. The formation of two cluster groups and the high membership coefficients within the groups pointed out the importance of further collection to cover more areas and more genetic variability within the species. The study also showed the lack of conservation activities; therefore, more attention from the appropriate organizations is needed to plan and implement natural and ex situ conservation activities.

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An improved method for genomic DNA extraction from strawberry leaves

Abstract: Several extraction methods of genomic DNA for identification and characterization of genetic diversity in

Cited by 35 publications

References 8 publications

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

Molecular characterization and population structure study of cambuci: strategy for conservation and genetic improvement

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