An Improved Method for In Vitro Large Scale Propagation of Piper betle L

Misra, Pratibha; Datta, Subodh Kumar; Johri, J. K.; Singh, Harpal; Srivastava, Alok Kumar

doi:10.1007/bf03263215

Cited by 4 publications

(7 citation statements)

References 5 publications

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“…Among the different explants nodal explants showed the best response towards shoot regeneration and subsequent plant development of P. betle (upper 2 -6 nodes). Some literature showed that nodal segments of upper 4 -5 nodes were suitable as the explants (Misra et al 2004). In the present study it was observed that inter-nodal segments did not show any sign of shoot regeneration, very few explants produced slight callus.…”

Section: Resultssupporting

confidence: 47%

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In vitro mass propagation of Piper betle L

et al. 2019

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An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best response on shoot/explants 13.2 ± 4.5 after 8 weeks of callus culture on MS medium with 0.5 mg/l BAP. The best response towards root induction was observed on half strength of MS with 0.25 mg/l IBA. The well rooted plants were successfully acclimatized and transferred to soil.

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Section: Resultssupporting

confidence: 47%

“…Some studies demonstrated that MS was a suitable medium for shoots and root induction in P. betle (Misra et al 2004). During this study, shoot regeneration was obtained through direct organogenesis from nodal explants using MS containing different hormonal supplements.…”

Section: Resultsmentioning

confidence: 99%

In vitro mass propagation of Piper betle L

et al. 2019

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“…Thus, culture medium with a suitable combination and concentration of auxin and cytokinins plays an important role in in vitro shoots multiplication. Misra et al (2004);Praveen (2005); Vidya et al (2005) had reported that, higher cytokinin concentration with lower auxin concentration is suitable for shoots multiplication and rooting in C. longa, which is analogous with the present finding. The effect of BAP alone or combination with NAA was also documented by Debergh and Zimmerman (1991) and suggested that herbaceous plants are highly responsive and most cultured herbaceous species produce robust, well-formed shoots suitable for further shoot proliferation to BAP treatments.…”

Section: Culture Initiation and Multiple Shoot Productionsupporting

confidence: 88%

“…The increase of BAP concentration higher than 8 mg L -1 or Kinetin higher than 4 mg L -1 suppressed the rate of shoot multiplication and shows stunted growth. In the present study, the number of shoots produced was much higher than previous reports by various workers (George, 1993;Misra et al, 2004;Nasirujjaman et al, 2005;Panda et al, 2007;Prathanturarug et al, 2003;Praveen, 2005;Rahman et al, 2004;Roy and Raychandhari, 2004;Sato et al, 1987; NAA using 250 ml phytajar 2001; Sunitibala et al, 2001;Vreugdenhil et al, 1998). Thus, highest multiplication rate (17.50±0.34) with average height of (13.02±0.27) was seen in Growtek (Fig.…”

Section: Culture Initiation and Multiple Shoot Productioncontrasting

confidence: 64%

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Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

et al. 2012

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Eleven cultivars of C. longa var. Lakadong were collected from Manipur having different topography. Curcumin content in different cultivars has been analyzed by using UV-Visible Spectrophotometer (100 Bio-Carry Spectrophotometer). The curcuminoids content were analyzed and quantified for identification of best quality cultivar. Thoubal Cultivar with highest curcumin content (9.44%) was subjected for tissue culture technique using different culture vessels and silver nitrate for rapid multiplication and scaling up of microrhizome production. High multiplication rate of 27.40±0.47 were obtained in Murashige and Skoog's medium supplemented with 3% sucrose + 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate. Effect of different culture vessels and silver nitrate were studied for microrhizome and multiple shoots formation. Relatively higher rate of shoots along with microrhizome (17.5±0.32) can be seen in Growtek which was grown without any plant growth regulator. Growtek was used for scaling up of microrhizome production in vitro and utmost microrhizome was produced in liquid Murashige and Skoog's medium supplemented with 8% sucrose, 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate (36.25±0.27). Addition of silver nitrate in the medium resulted in improvement of microrhizome induction in vitro. Higher concentration of silver nitrate (33, 44, 66, 88 µM) negatively affected the microrhizome and shoot multiplication and shows inhibition of tissue response completely. Analysis of in vitro derived plantlets during acclimatization shows that the exogenous applied of silver nitrate shows superior growth as compared to control. 90-95% of plantlets with and 75-80% plantlets without silver nitrate treatment were successfully established under ex vitro acclimatization. The protocol could be utilized for large scale production of true-to-type plantlets and as alternative method to step forward towards an improved commercial propagation system for more efficient and productivity.

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Clonal propagation of Rosa clinophylla Thory. through axillary bud culture

Misra

Chakrabarty

2009

Scientia Horticulturae

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An Improved Method for In Vitro Large Scale Propagation of Piper betle L

Cited by 4 publications

References 5 publications

In vitro mass propagation of Piper betle L

In vitro mass propagation of Piper betle L

Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

Clonal propagation of Rosa clinophylla Thory. through axillary bud culture

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