An improved method for monitoring active oxygen in bacteria-treated suspension cells using luminol-dependent chemiluminescence

Glazener, Judith A.; Orlandi, Elizabeth W.; Harmon, G. L.; Baker, C. Jacyn

doi:10.1016/0885-5765(91)90023-b

Cited by 59 publications

(29 citation statements)

References 16 publications

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Section: Ectocarpus Siliculosusmentioning

confidence: 99%

“…Following incubation the material was rinsed and placed in fresh half-strength modified at final concentrations of 100 µM. The concentration of hydrogen peroxide in the medium around algal thalli was determined using the luminol chemiluminescence method (Glazener et al 1991) with a LUMAT LB 9507 luminometer (EG&G Berthold, Bad Wildbach, Germany), as described before (Küpper et al 2001).For all further studies following the finding of PGA 2 as a potent inducer of the oxidative burst, a dose-response curve for the oxidative burst was measured using the aforementioned chemiluminescence assay, with final PGA 2 concentrations of 0.1, 1, 3, 6 and 10 µM, respectively. For analyses of free fatty acids and oxylipins, Laminaria thalli were incubated with PGA 2 at a final concentration of 10 µM.…”

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confidence: 99%

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Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Zambounis¹,

Gaquerel²,

Strittmatter³

et al. 2012

ALGAE

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We report an oxidative burst triggered by prostaglandin A 2 (PGA 2 ) in the brown algal kelp Laminaria digitata, constituting the first such discovery in an alga and the second finding of an oxidative burst triggered by a prostaglandin in a living organism. The response is more powerful than the oxidative burst triggered by most other chemical elicitors in Laminaria. Also, it is dose-dependent and cannot be inhibited by diphenylene iodonium, suggesting that another source than NAD(P)H oxidase is operational in the production of reactive oxygen species. Despite the very strong oxidative response, rather few effects at other levels of signal transduction pathways could be identified. PGA 2 does not increase lipolysis (free fatty acids) in Laminaria, and only one oxylipin (15-hydroxyeicosatetraenoic acid; 15-HETE) was found to be upregulated in Laminaria. In a subsequent set of experiments in the genome model Ectocarpus siliculosus, none of 5 selected candidate genes, all established participants in various stress responses, showed any significant differences in their expression profiles.Key Words: Cyclopentenone; diphenylene iodonium; Phaeophyta; polyunsaturated fatty acids; prostaglandin A 2 INTRODUCTIONBrown algae (Phaeophyta) make up most of the benthic biomass on temperate and polar rocky shores. Kelp forests are key ecosystems of such coastal areas and they constitute habitat and breeding grounds for a very large diversity of marine life forms -besides offering a physical protection by providing a wave energy-absorbing buffer for coasts. Brown algae belong to a lineage that has been evolving independently of other major photosynthetic Received December 20, 2011, Accepted February 23, 2012 *Corresponding Author E-mail: fkuepper@abdn.ac.uk Tel: +44-1224+44- -274490, Fax: +44-1224 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. (Reymond and Farmer 1998, Orozco-Cárdenas et al. 2001).The oxidative burst was initially discovered in human macrophages (Baldridge and Gerard 1933). It is now recognized also as a key element of plant (Wojtaszek 1997, Mittler et al. 2004) and algal , Dring 2005, Cosse et al. 2007) defense, where it is considered both as a rapid defense response and an internal emergency signal. It consists of the rapid and massive production of ROS such as superoxide (O 2 -), hydrogen peroxide (H 2 O 2 ), and hydroxyl radicals (• OH)which are likely produced via the interaction of plasma membrane-associated NAD(P)H oxidases (Wojtaszek 1997). Hence, pathogen attack triggers both the production of ROS and the oxidation cascade of fatty acids leading to the production of bioactive oxylipins in plants. In algal systems, the oxidative burst was initially discovered in response to injury in a red alga (Collén et al. 1994). Over the last decade, oxidative...

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Section: Ectocarpus Siliculosusmentioning

confidence: 99%

mentioning

confidence: 99%

Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Zambounis¹,

Gaquerel²,

Strittmatter³

et al. 2012

ALGAE

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“…One gram plants, initially with a DGR of 3'5-4"5% at c. 350 #mol photons m -z s -I, was exposed to 50-800 #mol photons m -2 s -x for an hour in an air-tight 50 ml glass bottle filled with NSW. H20 2 production was determined by luminol-dependent chemiluminescence (LDC) as detailed in Glazener et al (1991), with the exception that the buffer was 0"4 M MOPS (3[-N-morpholino]propanesulphonic acid, Sigma) and that an LKB 1250 luminometer equipped with a flat-bed recorder (Kipp & Zonen type BD 41, made in Holland) was used. To assay the effect of PPFD on HeO2 production, the algae were maintained at pH 8"2; to examine the effects of pH, the algae received 400 #mol photons m -z s -1.…”

Section: Hydrogen Peroxide Determinationmentioning

confidence: 99%

Destructive hydrogen peroxide production inEucheuma denticulatum(Rhodophyta) during stress caused by elevated pH, high light intensities and competition with other species

Mtolera

Collén

Pedersén

et al. 1995

European Journal of Phycology

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Growth of Eucheuma denticulatum was studied in the field and in laboratory experiments. Field co-cultivation of E, denticulatum with the green alga Ulva reticulata or the seagrass Thalassia sp. reduced daily growth rate (DGR) of a Tanzanian and a Philippine strain of E. denticulatum by I0-100% and 10-55%, respectively, depending upon the type of water current: a unidirectional water current produced the best growth. Laboratory co-cultivation of a Tanzanian strain of E. denticulatum with U. reticulata also reduced DGR (to 8% of the control) and nitrate-nitrogen uptake rate (to < 30% of the control) of E. denticu]atum and, moreover, it increased epiphytism of a red filamentous alga on E. denticulatum. E. denticulatum monoculture at pH 8"6 4-0"5 or at photosynthetic photon flux densities (PPFDs) higher than its growth optimum (350 4-50 #tool photons m -2 s -I) also increased epiphytism. The lack of a competitive mechanism for inorganic carbon uptake in Eucheuma may have contributed to its reduced growth during co-cultivation. During co-cultivation, elevated pH regimes (pH ~ 8"5) were created around the Eucheuma thalli as a result of photosynthesis, thus decreasing the concentration of CO 2 in the seawater to values around 1 #raM. As Eucheuma depends mainly on the CO 2 in the seawater for its growth, a higher pH can cause CO 2 limitation by decreasing CO2 concentration. Hydrogen peroxide (H202) production from the Tanzanian strain was also determined by luminol-dependent chemiluminescence. H202 production was found to increase with increased pH and PPFD (probably as a result of oxidative stress). Preincubation of plants with catalase for 5 min before addition of luminol prevented chemiluminescence, confirming H20 z as the substrate of the luminol reaction. We suggest that the inefficiency of E. denticulatum in HCO 3 utilisation contributes to its poor growth during field coexistence with seagrasses or Ulva sp. and that carbon deficiency induces H202 production in E. denticulatum.

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“…Indeed, the production of powerful oxidizing species such as OZ-. and H202 have been observed in plant cells soon after elicitor treatments (Aposto1 et al, 1989;Anderson et al, 1991;Arnott and Murphy, 1991;Vera-Estrella et al, 1992) or upon * Corresponding author; fax 33-42-25-46-56. challenge with pathogens (Chai and Doke, 1987;Adam et al, 1989;Baker et al, 1991;Glazener et al, 1991;Devlin and Gustine, 1992). In addition, it has been shown that severa1 cellular redox state-modifying compounds, such as sulfhydryl reagents, are able to elicit the accumulation of the isoflavonoid phytoalexins in clover callus (Gustine, 1981(Gustine, , 1987 and in soybean (Glycine max) hypocotyls (Stossel, 1984).…”

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confidence: 99%

Involvement of Oxidative Processes in the Signaling Mechanisms Leading to the Activation of Glyceollin Synthesis in Soybean (Glycine max)

1994

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l h e efficiency of hydroperoxides (tert-butyl hydroperoxide, hydrogen peroxide) and sulfhydryl reagents (iodoacetamide, p-chloromercuribenzene sulfonic acid) as glyceollin elicitors was examined in relation to sulfhydryl oxidation, or alteration, and to lipid peroxidation, i n 3-d-old soybean hypocotyl/radicle, Glycine max. lhese oxidative events were investigated as possible early steps i n the transduction mechanisms leading to phytoalexin synthesis. Free protein sulfhydryl groups were not modified after any of the eliciting treatments, thus indicating that immediate massive protein oxidation or modification cannot be considered a signal transduction step. Unlike sulfhydryl reagents, which led to a decrease of the free nonprotein sulfhydryl group (free np-SH) pool under all of the eliciting conditions, the results obtained with hydroperoxides indicated that immediate oxidation of the np-SH is not required for the signal transduction. Moreover, elicitation with 10 mM tertbutyl hydroperoxide did not lead to further oxidation or to changes i n np-SH leve1 during the critical phase of phenylalanine ammonialyase activation (the first 20 h), suggesting that np-SH modifications are probably not involved i n hydroperoxide-induced elicitation. O n the other hand, all treatments leading to significant glyceollin accumulation were able to trigger a rapid (within 2 h) lipid peroxidation process, whereas noneliciting treatments did not. In addition, transition metals, such as Fez+ and Cu+, were shown to stimulate both hydrogen peroxide-induced lipid peroxidation and glyceollin accumulation, again emphasizing that the two processes are at least closely linked in soybean. Among the oxidative processes triggered by activated oxygen species, oxidation of sulfhydryl compounds, or lipid peroxidation, our results suggest that lipid peroxidation i s sufficient to initiate glyceollin accumulation i n soybean. This further supports the hypothesis that lipid peroxidation could be involved as a step in the signal cascade that leads to induction of plant defenses.

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An improved method for monitoring active oxygen in bacteria-treated suspension cells using luminol-dependent chemiluminescence

Cited by 59 publications

References 16 publications

Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Destructive hydrogen peroxide production inEucheuma denticulatum(Rhodophyta) during stress caused by elevated pH, high light intensities and competition with other species

Involvement of Oxidative Processes in the Signaling Mechanisms Leading to the Activation of Glyceollin Synthesis in Soybean (Glycine max)

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An improved method for monitoring active oxygen in bacteria-treated suspension cells using luminol-dependent chemiluminescence

Cited by 59 publications

References 16 publications

Prostaglandin A2triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Prostaglandin A2triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Destructive hydrogen peroxide production inEucheuma denticulatum(Rhodophyta) during stress caused by elevated pH, high light intensities and competition with other species

Involvement of Oxidative Processes in the Signaling Mechanisms Leading to the Activation of Glyceollin Synthesis in Soybean (Glycine max)

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Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?