An improved method for the determination of 5‐hydroxymethylfurfural in Shenfu injection by direct analysis in real time‐quadrupole time‐of‐flight mass spectrometry

Abstract

The emergence of direct analysis in real time (DART) ion source provides the great possibility for rapid analysis of hazardous substance in drugs. DART mass spectrometry (DART-MS) enabled the conducting of a fast and non-contact analysis of various samples, including solid or liquid ones, without complex sample preparation or chromatographic separation. In this study, a modified DART-quadrupole time-of-flight mass spectrometry (DART-QTOF-MS) method was developed for identification and determination of 5-hydroxymethylfurfural (5-HMF) in Shenfu (SF) injection. The quantitative transfer of sample solution was introduced to the glass tips of DIP-it sampler at a fixed volume, which significantly increases the repeatability and accuracy of analytical results. The protonated ion of dibutyl phthalate in the atmosphere was used as the reference mass for TOF-MS recalibration during the data acquisition for constant high accuracy mass measurements. Finally, the developed DART-MS method was used to determine 5-HMF in seven batches of SF injection, and the contents of 5-HMF were not higher than 100 µg/mL. The results obtained were further confirmed by an ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometer (UHPLC-QQQ-MS). The overall results demonstrated that the DART-QTOF-MS method could be applied as an alternative technique for rapid monitoring 5-HMF in herbal medicine injection. Copyright © 2015 John Wiley & Sons, Ltd.

Efficacy and safety of Shenfu injection for the treatment of post-acute myocardial infarction heart failure: A systematic review and meta-analysis

“…Meyer (Araliaceae, Ginseng radix et rhizoma ) and Aconitμm carmichaelii Debx (Ranunculaceae, Aconiti radix ), is derived from the traditional Chinese medicine formula Shenfu decoction, which has been used in China for over hundreds of years. Several studies have reported the chemical profile of SFI using different methods; SFI mainly includes Aconitine alkaloids , Ginsenosaponin , Aconitum alkaloids , Ginsenoside , Aconitine , and Hydrophilic compound (for details, see Supplementary Table S1 ), among which ginsenosides and aconite alkaloids are identified as the main active ingredients of SFI ( Yang et al, 2014 ; Gao et al, 2016 ; Li et al, 2016 ).…”

Recent application of direct analysis in real time mass spectrometry in plant materials analysis with emphasis on traditional Chinese herbal medicine

“…Combined with the different MS data acquisition strategies, such as selected ion monitoring (SIM), multiple reaction monitoring (MRM), or parallel reaction monitoring (PRM), the extracted peak is more specific for the target ion, which could further improve the quantification accuracy. Li and coworkers analyze the 5‐hydroxymethylfurfural (5‐HMF) in Shenfu injection using DART‐Q‐TOF MS (Gao et al, 2016). By using EIC of 5‐HMF and internal standard of hamarline, good linearity ( R 2 = 0.996) and excellent performance for intra‐day and inter‐day precisions (RSD = 1.2% and 8.3%, respectively) were obtained using DART‐MS, and the results were comparable to that obtained by LC‐QQQ MS method.…”

“…Eight sampling replicates are shown for each of the following rates. Reproduced from Gao et al (2016) with permission from Wiley. [Color figure can be viewed at wileyonlinelibrary.com]…”

A Comprehensive and Rapid Quality Evaluation Method of Traditional Chinese Medicine Decoction by Integrating UPLC-QTOF-MS and UFLC-QQQ-MS and Its Application

“…Previous research has confirmed that the curative effect of SFPD is an integrative effect of ginsenosides and alkaloids [12,13,14]. Several methods for component analysis and determination of major constituents in ShenFu injection and their serum pharmacochemistry have been reported by using HPLC-PAD [15], UPLC-Q-TOF-MS [16,17], and HILIC-RPLC-MS/MS technologies [14]. Few papers have focused specifically on the decoction of ShenFu, either investigating pharmacochemistry [12] or just quantitative analysis for several constituents [18].…”