An improved perfusion fixation method for the testis

Forssmann, Wolf‐Georg; Ito, Susumu; Weihe, Eberhard; Aoki, Agustı́n; Dym, Martin; Fawcett, Don W.

doi:10.1002/ar.1091880304

Cited by 312 publications

(85 citation statements)

References 11 publications

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“…The sheep were perfused through the descending aorta with a mixture of polyvinylpyrolidinone, heparin, and procaine hydrochloride in saline to clear the blood from the vascular network, with the excess perfusate being drained from the inferior vena cava (Forssmann et al, 1977). Once the perfusate from the inferior vena cava was clear, four of the animals were perfused with an India ink/gelatin mixture (Miller and Jee, 1980) and the remaining six animals were perfused with an India ink/ formalin mixture (Hayat, 1981).…”

Section: Methodsmentioning

confidence: 99%

Microvascularization of the hypermineralized calcified fibrocartilage and cortical bone in the sheep proximal femur

et al. 2002

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It is well known that the incidence of hip fractures is increasing as the population ages, and that vascularity is one of the most important characteristics for any tissue (the proximal femur being no exception). Additionally, calcified fibrocartilage from tendon and ligament insertions comprises a significant portion of the fractional area of the proximal femur's cortical shell. The goal of the present investigation was to quantify and compare the microvascularity of the cortical bone and calcified fibrocartilage of the proximal femur in a sheep model. There were no regional differences in the vascular density of the cortical bone. However, the calcified fibrocartilage from tendon and capsular insertions were determined to be avascular, and regions of the proximal femur with insertions lacked a vascularized periosteum. If a vessel was present in the calcified fibrocartilage, it was located within an isolated region of bone tissue or osteoid. Since blood vessels appear to be a significant contributor to the health and remodeling of mineralized tissue, it is hypothesized that the large areas of avascular calcified fibrocartilage present on the elderly femoral neck may predispose these regions to damage accumulation. Therefore future research should examine the role of the vascularity to the proximal femur in the mechanisms of numerous pathological conditions, such as avascular necrosis, osteopenia, and hip fractures.

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Section: Methodsmentioning

confidence: 99%

Microvascularization of the hypermineralized calcified fibrocartilage and cortical bone in the sheep proximal femur

et al. 2002

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“…A canula of suitable size was inserted into the straight intratunical portion of the testicular artery and a rinsing fluid (for composition see WROBEL et al, 1978) was instilled at low pressure. One ovine testis was fixed with Bouin's fluid; all other testes were fixed with solution I and II of FORSSMANN et al (1977). Cube-shaped blocks with a square length of about 1 mm were cut from the tissue and postfixed in solution II.…”

Section: Methodsmentioning

confidence: 99%

Immunocytochemical Demonstration of Cytoskeletal Proteins in Seminiferous Tubules of Adult Rams and Bulls.

Steger

Schimmel

Wrobel

1994

Archives of Histology and Cytology

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“…Immediately after slaughter in Assiut (Egypt) abattoir, the testes were removed and fixed by vascular perfusion via the straight intratunical portion of the testicular artery. Following a rinsing procedure, the fixatives I and II of FORSSMANN et al (1977) were instilled at low, steady pressure (for details see WROBEL et al, 1978WROBEL et al, , 1988. Small blocks from seminiferous parenchyma were postfixed in solution II for 3-5h, washed in 0.2M phosphate buffer, osmicated (1% Os 04), dehydrated in a graded series of ethanol and embedded in ERL (SPURR, 1969).…”

Section: Methodsmentioning

confidence: 99%