Quantitative Aspects of Water Buffalo (Bubalus bubalis) Spermatogenesis.

Pawar, Harcharan Singh; Wrobel, Karl‐Heinz

doi:10.1679/aohc.54.491

Cited by 14 publications

(8 citation statements)

References 32 publications

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“…Furthermore, there are no desmosomes or tight junctions between adjacent Sertoli cells beyondtheSertoli-Sertolijunctions dividing thebasal andtheadluminal tubularcompartment; as a consequence all intercellular spaces in the adluminal tubular compartment communicate freely with the tubular lumen. Also for other ruminant species a heavy cell loss is reported during meiosis I1 (Bilaspuri and Guraya, 1980;Pawar, 1991). Sertoli cells occupy 3 1.4. to 38.3% of the tubular epithelium in the fallow deer, depending on stage of cycle.…”

Section: Discussionmentioning

confidence: 93%

“…Initially also the spermatogeniccycleof thefallow deer was divided into 8 stages with accomplished spermiation as demarcation between two cycles. However, since stages 5 , 6 and 7 are of short duration and do not reveal significant differences, the findings of stages 5-7 in the definition of Ekstedt et al (1986) were sum-marized, a procedure that has been already successfully applied in quantitative studies of bovine (Wrobel and Schimmel, 1989) and water buffalo (Pawar andWrobel, 1990a, 1990b) seminiferous epithelium. The duration of the single stages is reflected by their frequency in histological sections.…”

Section: Methodsmentioning

confidence: 99%

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Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Wrobel

Kessler

Schimmel

1993

Reprod Domestic Animals

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Contents: The quantitative morphology of the seminiferous epithelium was studied in 10 testes of 16‐ to 17‐monthsold fallow bucks (Dama dama). Seminiferous tubules constitute 77.2% of the testicular parenchyma and amount to a total length of 400–750 m per testis. According to varying cell associations 6 different stages can be observed during the spermatogenic cycle. The average tubular diameter varies between 215 μm (stage 1) and 230 μm (stage 8), the epithelial height between 67.6 μm in stage 3 and 71.9 μm in stage 8 when spermiation occurs. From primary spermatocytes during the prophase of the first meiotic division preleptotenes have smallest nuclear (203.7 μm3) and cellular (469.1 μm3) volumes, whereas late diplotenes show a nuclear volume of 846.7μm3 and a cellular volume of 3440.5 μm3, that is an increase of more than 4 times for the nucleus and more than 7 times for the cell body. Numerical analysis reveals 4 spermatogonial divisions and a considerable loss of spermatogenic cells during the second meiotic division and subsequent spermiogenesis. Sertoli cells constitute between 31.4% (stage 4) and 38.3% (stage 1) of the tubular epithelium. The calculated cell volume of an individual Sertoli cell increases during every cycle from 6515.3 μm3 instage 3 to 8350.0 μm3 in stage 8; that is a volume change of 22%. The Sertoli cell organelle with most prominent cyclical variations is the endoplasmic reticulum. A lipid cycle is absent in the seminiferous epithelium of the fallow deer. Inhalt: Quantitative Studien am Keimepithel des männlichen Damwilds (Dama dama). Das Keimepithel im Hoden von fünf 16 bis 17 Monate alten Damwildjährlingen (Dama dama) wurde mit morphometrischen Methoden untersucht. Tubuli seminiferi machen 77,2% des Hodenparenchyms aus; die Gesamttubuluslange pro Hoden betragt zwischen 400 und 750 m. Sechs Spermatogenesestadien können beim Damhirsch identifiziert werden, wobei für diese Einteilung wechselnde Zellassoziationen im Keimepithel zugrunde gelegt wurden. Der Durchmesser der Tubuli seminiferi wechselt zwischen 215 μm (Phase 1)und 230 μm (Phase 8). Die Tubulusepithelhöhe schwankt ebergfalls zwischen 67,6 μm in Phase 3 und 71,9 μm in Phase 8 (Spermiation). Präleptotäne habendie kleinsten Kern‐ und Zellvolumina (203,7 μm3 bzw. 469,1 μm3) von allen Spermatozyten I. Ordnung, Diplotäne aus Phase 4 die größten Werte (846,7 μm3 bzw. 3440,5 μm3), das ist ein Zuwachs um das Vierfache bei mKern und um das Siebenfache beim Zeilleib. Eine numerische Anaiyse derZellzahlen in Tubulusquerschnitten aus den verschiedenen Phasen der Spermatogenese erlaubt die Schlußfolgerung, daß es 4 Spermatogonienteilungen bei dieser Spezies gibt, und daß eine beträchtliche Anzahl aller rechnerisch möglichen Keimzellen während der Spermatokenese verloren geht, vor allem während der zweiten Reifeteilung und der Spermiogenese. Sertolizellen machen zwischen 31,4 (Phase 4) und 38,3% (Phase 1) des Keimepithels aus. Eine Sertolizelle aus Phase 3 besitzt ein rechnerisches Zellvolumen von 6515,3 μm3, eine Zelle aus Phase 8 ein Zell...

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Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Wrobel

Kessler

Schimmel

1993

Reprod Domestic Animals

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“…Little is known regarding the function of these nuclear modifications but they may provide a unique site for nuclear targeting of specific proteins (Rothbarth et al ., ). Other unique aspects of the Sertoli cell nucleus include a high density of nuclear pores that varies depending on the stage of spermatogenesis (Cavicchia et al ., ) and heavy vesiculation of the nucleoplasm in some species (Pawar & Wrobel, ).…”

Section: Morphology Of the Sertoli Cellmentioning

confidence: 99%

The Sertoli cell: one hundred fifty years of beauty and plasticity

et al. 2016

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SUMMARY It has been one and a half centuries since Enrico Sertoli published the seminal discovery of the testicular ‘nurse cell’, not only a key cell in the testis, but indeed one of the most amazing cells in the vertebrate body. In this review, we begin by examining the three phases of morphological research that have occurred in the study of Sertoli cells, because microscopic anatomy was essentially the only scientific discipline available for about the first 75 years after the discovery. Biochemistry and molecular biology then changed all of biological sciences, including our understanding of the functions of Sertoli cells. Immunology and stem cell biology were not even topics of science in 1865, but they have now become major issues in our appreciation of Sertoli cell’s role in spermatogenesis. We end with the universal importance and plasticity of function by comparing Sertoli cells in fish, amphibians, and mammals. In these various classes of vertebrates, Sertoli cells have quite different modes of proliferation and epithelial maintenance, cystic vs. tubular formation, yet accomplish essentially the same function but in strikingly different ways.

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“…Whereas preleptotene spermatocytes during the short premeiotic S-phase (stage 1) have positive nuclei, the MIB-IR disappears completely during leptotene and zygotene. This absence of MIB-expression may be the consequence of a change in intranuclear topography typical for ruminants (Pawar & Wrobel, 1991). From leptotene through zygotene the paired chromosomes are displaced to one side of the nucleus, leaving the other half empty.…”

Section: Discussionmentioning

confidence: 99%

Expression of the proliferation-associated Ki-67 antigen in bovine testicular tubular cells during the seminiferous epithelial cycle, demonstrated with the MIB-1 antibody

2009

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Ki-67 expression in the seminiferous tubule of the bovine testis was studied by immunohistochemistry during the seminiferous epithelial cycle using the monoclonal antibody MIB-1. Spermatogonial proliferation is most obvious in stages 5-7, and 8, when B-spermatogonia divide. A lower rate of spermatogonial propagation is observed preceding or during meiosis in stages 1-4. The MIB-1 antibody also gives positive results with some post-spermatogonial tubular cells. Preleptotenes passing through S-phase in stage 1 reveal positive nuclei. During prophase of meiosis I pachytenes react strongly, diplotenes react in an attenuated manner, while leptotenes and zygotenes stay negative. Secondary spermatocytes seen in stage 4 are positive as are the chromosomes during meta-and anaphase of the meiotic divisions. Post-meiotic spermatids are also decorated but stop Ki-67 expression abruptly at the end of stage 4. Sertoli cells are negative.

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Quantitative Aspects of Water Buffalo (Bubalus bubalis) Spermatogenesis.

Cited by 14 publications

References 32 publications

Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

The Sertoli cell: one hundred fifty years of beauty and plasticity

Expression of the proliferation-associated Ki-67 antigen in bovine testicular tubular cells during the seminiferous epithelial cycle, demonstrated with the MIB-1 antibody

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