Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Abstract

Contents: The quantitative morphology of the seminiferous epithelium was studied in 10 testes of 16‐ to 17‐monthsold fallow bucks (Dama dama). Seminiferous tubules constitute 77.2% of the testicular parenchyma and amount to a total length of 400–750 m per testis. According to varying cell associations 6 different stages can be observed during the spermatogenic cycle. The average tubular diameter varies between 215 μm (stage 1) and 230 μm (stage 8), the epithelial height between 67.6 μm in stage 3 and 71.9 μm in stage 8 when spermiation occurs. From primary spermatocytes during the prophase of the first meiotic division preleptotenes have smallest nuclear (203.7 μm3) and cellular (469.1 μm3) volumes, whereas late diplotenes show a nuclear volume of 846.7μm3 and a cellular volume of 3440.5 μm3, that is an increase of more than 4 times for the nucleus and more than 7 times for the cell body. Numerical analysis reveals 4 spermatogonial divisions and a considerable loss of spermatogenic cells during the second meiotic division and subsequent spermiogenesis. Sertoli cells constitute between 31.4% (stage 4) and 38.3% (stage 1) of the tubular epithelium. The calculated cell volume of an individual Sertoli cell increases during every cycle from 6515.3 μm3 instage 3 to 8350.0 μm3 in stage 8; that is a volume change of 22%. The Sertoli cell organelle with most prominent cyclical variations is the endoplasmic reticulum. A lipid cycle is absent in the seminiferous epithelium of the fallow deer. Inhalt: Quantitative Studien am Keimepithel des männlichen Damwilds (Dama dama). Das Keimepithel im Hoden von fünf 16 bis 17 Monate alten Damwildjährlingen (Dama dama) wurde mit morphometrischen Methoden untersucht. Tubuli seminiferi machen 77,2% des Hodenparenchyms aus; die Gesamttubuluslange pro Hoden betragt zwischen 400 und 750 m. Sechs Spermatogenesestadien können beim Damhirsch identifiziert werden, wobei für diese Einteilung wechselnde Zellassoziationen im Keimepithel zugrunde gelegt wurden. Der Durchmesser der Tubuli seminiferi wechselt zwischen 215 μm (Phase 1)und 230 μm (Phase 8). Die Tubulusepithelhöhe schwankt ebergfalls zwischen 67,6 μm in Phase 3 und 71,9 μm in Phase 8 (Spermiation). Präleptotäne habendie kleinsten Kern‐ und Zellvolumina (203,7 μm3 bzw. 469,1 μm3) von allen Spermatozyten I. Ordnung, Diplotäne aus Phase 4 die größten Werte (846,7 μm3 bzw. 3440,5 μm3), das ist ein Zuwachs um das Vierfache bei mKern und um das Siebenfache beim Zeilleib. Eine numerische Anaiyse derZellzahlen in Tubulusquerschnitten aus den verschiedenen Phasen der Spermatogenese erlaubt die Schlußfolgerung, daß es 4 Spermatogonienteilungen bei dieser Spezies gibt, und daß eine beträchtliche Anzahl aller rechnerisch möglichen Keimzellen während der Spermatokenese verloren geht, vor allem während der zweiten Reifeteilung und der Spermiogenese. Sertolizellen machen zwischen 31,4 (Phase 4) und 38,3% (Phase 1) des Keimepithels aus. Eine Sertolizelle aus Phase 3 besitzt ein rechnerisches Zellvolumen von 6515,3 μm3, eine Zelle aus Phase 8 ein Zell...

Correlation of histological and histometric changes in rats testes treated with chloroquine phosphate

“…The negative correlation observed between the lumen and seminiferous tubules in the control group was expected. This supports the work of Wrobel et al, 1998. Positive correlation observed in the test groups indicates a deviation from the normal that shows that the test drug had effects on these groups.…”

Histological organization of roe deer testis throughout the seasonal cycle: variable and constant components of tubular and interstitial compartment

“….005w>The general histology of active roe deer testis is similar to most mammals, especially in comparison with other ruminants such as cattle and sheep (Swierstra 1966;Ortavant et al 1977;Wrobel et al 1995) or cervids such as red deer (Hochereau-de Reviers and Lincoln 1978) and fallow deer (Wrobel et al 1993), as has also been shown in earlier qualitative descriptions (Stieve 1950;Short and Mann 1966;Marchlewsaka-Koij and Kruczek 1988;Gentile et al 1989). During active spermatogenesis, volume proportions of the tubular and interstitial compartment are in the range reported for many other mammalian species (Russell at al.…”

Immunocytochemical Demonstration of Cytoskeletal Proteins in Seminiferous Tubules of Adult Rams and Bulls.

“…During the seminiferous epithelial cycle, Sertoli cells undergo extensive changes in volume, cell shape and internal organization (RUSSELL et al, 1981;VOGL and SOUCY, 1985;AMLANI and VOGL, 1988;WROBEL and SCHIMMEL, 1989;SINGHPAWAR and WROBEL,1991;WROBEL et al, 1993), which all involve also their cytoskeletal elements. Furthermore, cytoskeletal filaments participate in the organization of the blood-testis-barrier, in the formation of contact between Sertoli and spermatogenic cells, in vertical germ cell transport and in spermiation.…”

“…For the present study the first classification method was adopted with 8 stages and accomplished spermiation as a demarcation between the two cycles. However, since stages 5, 6 and 7 are of short duration, these were combined into one stage 5-7, as has already been done in quantitative studies of the tubular epithelium of other ruminants (WROBEL and SCHIMMEL, 1989;SINGHPAWAR and WROBEL, 1991;WROBEL et al, 1993).…”