An improved process for cell disruption and astaxanthin extraction from Phaffia rhodozyma

Abstract

Phaffia rhodozyma is one of the most important natural sources of the carotenoid astaxanthin, and the key process for extracting intracellular astaxanthin is disrption of the thick cell wall. In this work, an improved process for cell disruption and astaxanthin extraction from Phaffica rhodozyma was studied using an autoclave at low acid concentration. Under the optimum conditions (HCl 0.5 M and autoclave pressure 0.1 Mpa, 15 min), the relative residual astaxanthin and astaxanthin extractability reached 90.4 ± 3.5% and 84.8 ± 3.2%, respectively. The scanning electron microscopy pictures showed that all yeast cells shattered into fragments after autoclave treatment at low acid concentration condition, whereas cells were intact or partly broken after treatment by some other physical and chemical processes. This new method left no residual toxin and gavehigher extraction recovery, with good prospects for industrial use.

Production and stability of water‐dispersible astaxanthin oleoresin from Phaffia rhodozyma

“…The pigment source, the pretreatment temperature, the time, the solid–liquid ratio and the solvent used in the extraction process are the principal variables that affect the yield of astaxanthin extraction. Some incomplete pretreatments have been developed for the extraction of astaxanthin from P. rhodozyma (Xiao et al ., ), H. pluvialis (Mendes‐Pinto et al ., ) and Rhodotorula glutinis (Park et al ., ). In this study, the effects of both the pretreatment and the solvent on the pigment extraction yield from P. rhodozyma were determined, and the results are shown in Table .…”

Astaxanthin as feed supplement in aquatic animals

“…High astaxanthin yield was also noted in different studies employing hydrochloric acid pretreatment (Mendes-Pinto et al 2001;Dong et al 2014). Acidic method was also optimized for the isolation of astaxanthin from P. rhodozyma in a few studies (Ni et al 2008;Xiao et al 2009). Zou et al (2013) recorded a relatively high astaxanthin extraction yield of 18 mg g À1 from dried H. pluvialis biomass using ethanol: ethyl acetate (1:1 v/v) mixture with a brief processing period (2 h).…”

Study on the relationship between intracellular metabolites and astaxanthin accumulation during Phaffia rhodozyma fermentation

“…The astaxanthin-producing Phaffia rhodozyma has great industrial potential for natural astaxanthin production with such advantages as fast breeding, short growth cycle, and mature fermentation process [6,7,8]. However, the production of natural astaxanthin cannot meet the market demand, but a large majority of the commercial supply (approximately 97%) is synthetic astaxanthin [9].…”