An Improved Protocol for Micropropagation of Jojoba (Simmondsia chinensis (Link) Schneider)

Abstract

In vitro consecutive micropropagation stages of Simmondsia chinensis was studied and a micropropagation protocol was developed by Four media were tested Murashige and Skoog (MS), Schenk and Hildebrandt (SH), Driver and Kuniyuk (DKW) and Lloyd and McCown (Woody plant medium, WPM) with shoot-tip and one node cutting for the establishment stage. Murashige and Skoog (MS) was found to be the best medium and one node cutting as starting material for establishment from mother plants. Multiple shoots were obtained on MS medium supplemented with1.00 mgl-1 6-benzyladenine (BA) in the combination with1.00 mgl-1 Indole-3-acetic acid (IAA). High frequency of rooting was obtained on full MS solid medium supplemented with 7.00 mgl-1 Indole-3-butyric acid (IBA) alone. Regenerated plantlets were successfully transferred to pots containing mixture of peat moss, vermiculite and sand at equal volume with 90% survival.