An improved reference method for serum cations measurement by ion chromatography

Zou, Jihua; Shen, Min; Zhang, Man; Tu, Minmin; Feng, Rui; Yan, Ying; Zou, Bingde

doi:10.1002/jcla.22429

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…In the light of the problem, a more suitable measurement procedure should be developed to apply to more laboratories. According to the use of ion chromatography (IC) reference measurement procedures to determine cations in human serum reported in the previous literature, 8‐11 the measurement precisions obtained are comparable to those achieved in clinical chemistry using other reference procedures and are approximately in the range of 1% 12 …”

Section: Introductionmentioning

confidence: 71%

Ion chromatography as candidate reference method for the determination of chloride in human serum

Shen

Tu²,

Zhang³

et al. 2020

Clinical Laboratory Analysis

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Background We developed an ion chromatography (IC) method for measurement of chloride in human serum which was regarded as a simple, rapid, accurate, and sensitive technique. The method will be hopefully selected as a candidate reference method. Method Serum aliquots of 0.1 mL were diluted 500 times with Milli‐Q water, and chloride in serum samples was measured by IC with a gradient elution procedure using a KOH eluent generator. Results Based on the data, chloride in human serum was well detected by IC. The calibration curve for chloride was linear in the concentration range from 0 to 0.42 mmol/L with a correlation coefficient of .99995 under the optimum experimental conditions. The chloride concentration had a good linear relationship with the peak areas of chloride. This method was sensitive because of the low limit of detection (LOD) and the low limit of quantification (LOQ) 9.87 × 10 −5 mmol/L and 3.27 × 10 −4 mmol/L, respectively. Besides, the method was highly precise with the within‐run coefficient of variations (CVs) for the measurement of low, medium, and high concentration level samples 0.32%, 0.73%, and 0.50%. As for the evaluation of accuracy, the biases were less than ±1% and 2% by comparing with National Institute of Science and Technology (NIST) standard material SRM 956d and 2013‐2018 IFCC‐RELA samples, respectively. Finally, the biases between IC method and the inductively coupled plasma mass spectrometry (ICP‐MS) method were less than 1% which showed good agreement. Conclusion Ion chromatography is a simple sample treatment procedure for the determination of chloride in human serum with high sensitivity and specificity. The proposed method could be recommended as a candidate reference method for the determination of serum chloride in human serum.

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Section: Introductionmentioning

confidence: 71%

Ion chromatography as candidate reference method for the determination of chloride in human serum

Shen

Tu²,

Zhang³

et al. 2020

Clinical Laboratory Analysis

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“…Consequently, a sensitive, cost-effective, rapid and easy operation method for H 2 O 2 determination would be demanded for bioassays and environmental applications [ 3 ]. Up to now, several techniques for H 2 O 2 determination, such as chromatography [ 4 ], chemiluminescence, electrochemistry [ 5 , 6 ] and colorimetric method [ 7 ], have been reported. Among these techniques, colorimetric route has several outstanding advantages, including visibility, low cost, easy automation, portability and operation convenience [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

A facile preparation of FePt-loaded few-layer MoS2 nanosheets nanocomposites (F-MoS2-FePt NCs) and their application for colorimetric detection of H2O2 in living cells

Dai

et al. 2019

J Nanobiotechnol

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BackgroundRapid and sensitive detection of H2O2 especially endogenous H2O2 is of great importance for series of industries including disease diagnosis and therapy. In this work, uniform FePt nanoparticles are successfully anchored onto Few-layer molybdenum disulfide nanosheets (F-MoS2 NSs). The powder X-ray diffraction, transmission electron microscopy, UV–Vis spectra and atomic force microscopy were employed to confirm the structure of the obtained nanocomposites (F-MoS2-FePt NCs). The prepared nanocomposites show efficient peroxidase-like catalytic activities verified by catalyzing the peroxidation substrate 4,4′-diamino-3,3′,5,5′-tetramethylbiphenyl (TMB) with the existence of H2O2.ResultsThe optimal conditions of the constructed colorimetric sensing platform is proved as 35 °C and pH 4.2. Under optimal catalytic conditions, the detection limit for H2O2 detection reaches 2.24 μM and the linear ranger is 8 μM to 300 μM. Furthermore, the proposed colorimetric sensing platform was successfully utilized to detect the intracellular H2O2 of cancer cells (MCF-7).ConclusionsThese findings indicated that the F-MoS2-FePt-TMB-H2O2 system provides a potential sensing platform for hydrogen peroxide monitoring in living cells.Electronic supplementary materialThe online version of this article (10.1186/s12951-019-0465-3) contains supplementary material, which is available to authorized users.

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Determination of nutrient concentration in liquid culture of cyanobacteria Nostoc sp. by capillary electrophoresis and inductively coupled plasma mass spectrometry

Melicherová,

Vaculovič,

Kočí

et al. 2024

Analytical Biochemistry

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An improved reference method for serum cations measurement by ion chromatography

Abstract: Ion chromatography with a simple sample treatment procedure for the determination of cations in human serum with high sensitivity and specificity was developed. The proposed method could be recommended as a candidate reference method for the determination of serum cations.

Cited by 5 publications

References 18 publications

Ion chromatography as candidate reference method for the determination of chloride in human serum

Ion chromatography as candidate reference method for the determination of chloride in human serum

A facile preparation of FePt-loaded few-layer MoS2 nanosheets nanocomposites (F-MoS2-FePt NCs) and their application for colorimetric detection of H2O2 in living cells

Determination of nutrient concentration in liquid culture of cyanobacteria Nostoc sp. by capillary electrophoresis and inductively coupled plasma mass spectrometry

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