An improved spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation

Sun, Ming; Zigman, Seymour

doi:10.1016/0003-2697(78)90010-6

Cited by 724 publications

(329 citation statements)

References 6 publications

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“…33 Superoxide dismutase was assayed according to the method of Sun and Zigma 1978 34 while catalase was evaluated as reported by Aebi, 1984. 35 Reduced glutathione was measured as described by Sedlak and Lindsay 1968 36 while malondialdehyde was evaluated according to Buege and Aust 1978.…”

Section: Evaluation Of Renal Function and Kidney Oxidative Stress Indmentioning

confidence: 99%

Prospects of N-Acetylcysteine and Melatonin as Treatments for Tramadol-Induced Renal Toxicity in Albino Rats

Adikwu¹,

Bokolo²

2017

Pharm Sci

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Section: Evaluation Of Renal Function and Kidney Oxidative Stress Indmentioning

confidence: 99%

Prospects of N-Acetylcysteine and Melatonin as Treatments for Tramadol-Induced Renal Toxicity in Albino Rats

Adikwu¹,

Bokolo²

2017

Pharm Sci

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“…Catalase activity was quantified according to Sinha (1972) and the SOD activity was quantified according to Sun and Zigman (1978).…”

Section: Catalase and Sodmentioning

confidence: 99%

Oxidative Status of Boar Semen during Storage

Zanella¹,

Zanella²,

Poetini³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Abstract:The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 µM. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen.

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“…Catalase (CAT) activity was determined according to Beers and Sizer (1952), superoxide dismutase (SOD) activity was determined by the method of Sun and Zigman (1978), and glutathione peroxidase (GPx) activity was determined with method of Rotruck et al (1973). Lipid peroxidation in the liver was measured by the thiobarbituric acid-reactive method according to Kimura et al (1981).…”

Section: Biochemical Assaysmentioning

confidence: 99%

Antioxidative and Hepatoprotective Effects ofPhysalis peruvianaExtract against Acetaminophen-Induced Liver Injury in Rats

Chang

Lin

et al. 2008

Pharmaceutical Biology

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The aim of this study was to examine the antioxidant activities of Physalis peruviana L. (Solanaceae) aqueous extract (PPWE) and its protective effect against acetaminophen (APAP)-induced hepatotoxicity in rats. Using different models of antioxidant assay, namely ferric thiocyanate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and reducing power, PPWE showed a dose-dependent increase in antioxidant activities, with total antioxidant activity (IC 50 : 0.81 µg/ml) close to that of vitamin C (IC 50 : 0.89 µg/ml). APAP at 850 mg/kg significantly increased the levels of serum glutamic pyruvic transaminase (sGPT), glutamic oxaloacetic transaminase (sGOT) and alkaline phosphatase (sALP). However, pre-treatment with PPWE at doses150, 300, and 600 mg/kg body weight significantly prevented the increase in these enzymes, which are the major indicators of liver hepatitis. Biochemical assays of liver homogenate showed that PPWE at 150∼600 mg/kg significantly enhanced superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) concentrations, and diminished the level of thiobarbituric acid reactive substances (TBARS). Furthermore, liver histological observation also showed an obvious amelioration in the liver cell necrosis, liver lesion, and fatty changes in PP-treated groups. High performance liquid chromatographic analysis showed that ellagic acid (ca. 0.2%) but not others could be the major component contributing to the antioxidant and hepatoprotective activities of PPWE. The present study concludes that PPWE possesses antioxidant activity and potent hepatoprotective effect against APAP-induced liver injury in rats.

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An improved spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation

Cited by 724 publications

References 6 publications

Prospects of N-Acetylcysteine and Melatonin as Treatments for Tramadol-Induced Renal Toxicity in Albino Rats

Prospects of N-Acetylcysteine and Melatonin as Treatments for Tramadol-Induced Renal Toxicity in Albino Rats

Oxidative Status of Boar Semen during Storage

Antioxidative and Hepatoprotective Effects ofPhysalis peruvianaExtract against Acetaminophen-Induced Liver Injury in Rats

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