Antioxidative and Hepatoprotective Effects ofPhysalis peruvianaExtract against Acetaminophen-Induced Liver Injury in Rats

Abstract

The aim of this study was to examine the antioxidant activities of Physalis peruviana L. (Solanaceae) aqueous extract (PPWE) and its protective effect against acetaminophen (APAP)-induced hepatotoxicity in rats. Using different models of antioxidant assay, namely ferric thiocyanate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and reducing power, PPWE showed a dose-dependent increase in antioxidant activities, with total antioxidant activity (IC 50 : 0.81 µg/ml) close to that of vitamin C (IC 50 : 0.89 µg/ml). APAP at 850 mg/kg significantly increased the levels of serum glutamic pyruvic transaminase (sGPT), glutamic oxaloacetic transaminase (sGOT) and alkaline phosphatase (sALP). However, pre-treatment with PPWE at doses150, 300, and 600 mg/kg body weight significantly prevented the increase in these enzymes, which are the major indicators of liver hepatitis. Biochemical assays of liver homogenate showed that PPWE at 150∼600 mg/kg significantly enhanced superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) concentrations, and diminished the level of thiobarbituric acid reactive substances (TBARS). Furthermore, liver histological observation also showed an obvious amelioration in the liver cell necrosis, liver lesion, and fatty changes in PP-treated groups. High performance liquid chromatographic analysis showed that ellagic acid (ca. 0.2%) but not others could be the major component contributing to the antioxidant and hepatoprotective activities of PPWE. The present study concludes that PPWE possesses antioxidant activity and potent hepatoprotective effect against APAP-induced liver injury in rats.

Antioxidant Capacity and Metal Content of Physalis peruviana L. Fruit Sold in Markets

“…Demir et al [7] also found similar results that the antioxidant activity of P. peruviana fruit, from a local supplier from Antalya, using the DPPH radical scavenging method determined the IC 50 values of ascorbic acid and P. peruviana were calculated as 1.06 µg/ml and 430 µg/ml, respectively. Chang et al [8] suggest that the aqueous extract of P. peruviana has antioxidant activity in a concentration-dependent manner. When compared with vitamin C (for the 5 µg/ml, scavenging 94.64 %), their extract concentrations 50 µg/ml, 100 µg/ml, and 300 µg/ml showed the scavenging 13.17 %, 22.04 %, 52.72 %, respectively.…”

“…P. peruviana is a medicinal plant widely used in folk medicine for treating diseases such as malaria, asthma, hepatitis, dermatitis, cancer, diuretic, rheumatism [3,7]. The fruit of P. peruviana may be considered as good sources of natural antioxidants for medicinal uses [8,9]. Fruits are valuable sources of nutritional elements such as magnesium (Mg), calcium (Ca), sodium (Na), potassium (K), phosphorus (P), iron (Fe), zinc (Zn) [10].…”

Antioxidant activity and free radical-scavenging of cape gooseberry (Physalis peruviana L.) in hepatocellular carcinoma rats model

“…Additionally, the observed increase in alterations to liver enzymes, including ALT, AST and ALP, in HCCreceived CHJ rats may be due to the improvement of the functional status of hepatocytes with preservation of cellular architecture leakage of intracellular enzymes through membrane-stabilizing activity. [18,53] Previous studies have suggested that CGJ is a significant source of natural antioxidative compounds.…”

“…[ [12][13][14] Cape gooseberry extracts show antioxidant activity, [15,16] anti-inflammatory activity, [17,18] and anti-hepatotoxic [18] and antiproliferative effects on hepatoma cells. [19] This fruit also has excellent potential as a food-based strategy for anti-diabetic and anti-hypertensive products.…”

Exploring the potential of an Andean fruit : an interdisciplinary study on the cape gooseberry (Physalis peruviana L.) value chain

“…A number of studies has been conducted to test health properties of Physalis peruviana L plant, cape gooseberry and calyx, concluding to a potential association of intakes with in vitro and in vivo anti-inflammatory activity [25,26], inhibition of TPA-induced (12-O-tetradecanoylphorbol-13-acetate) oedema in mice [27], effectiveness in decreasing cadmium toxicity in rats [28,8,29], hepatoprotective, renoprotective and cholesterol suppression effects [8,[30][31][32][33][34], antidiabetic and antihypertension activity [35][36][37][38][39] and cytotoxic activity against in vitro non-specific cancer cells [40] and lung cancer cells [41]. These potential health benefits are associated with the presence of phytochemicals in the extracts such as withanolides, physalins, vitamin C, flavonoids and phenolics, among others.…”