An improvised in vitro vegetative propagation technique for Bambusa tulda: influence of season, sterilization and hormones

Bhadrawale, Deepti; Mishra, Jay Prakash; Mishra, Yogeshwar

doi:10.1007/s11676-017-0569-2

Cited by 12 publications

(12 citation statements)

References 15 publications

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“…Results of in vitro establishment of Bambusa vulgaris (Figure 3) are similar to those found in B. tulda (Bhadrawale et al, 2018), G. chacoensis (Ornellas et al, 2019) and B. oldhami (Silveira et al, 2020). Thus, different responses of B. vulgaris explants were observed, varying according to the months of collection and origin.…”

Section: In Vitro Establishmentsupporting

confidence: 74%

“…Tissue contamination is an undesirable aspect of the in vitro introduction process. Fungal contamination is reported in several bamboo species, as well as Bambusa tulda (Bhadrawale et al, 2018), B. vulgaris (Furlan et al, 2018;Ribeiro et al, 2020), Drepanostachyum luodianense (Lin et al, 2019), Guadua angustifolia (Nogueira et al, 2019), Guadua chacoensis (Ornellas et al, 2019), Guadua magna (Nogueira et al, 2019) and Guadua latifolia (Leão et al, 2020). However, the results showed differences between stock plant cultivated in different environments (A1, A2, and A3) and shoot collection (SC1, …, SC9) factors at 30 days of in vitro cultivation.…”

Section: In Vitro Establishmentmentioning

confidence: 99%

“…There are traditional, ecological, and industrial applications to bamboo plants, such as food, carbon sequestration and cellulose production (Ramakrishnan et al, 2020;Mustafa et al, 2021). Because of high plasticity regarding climate conditions, bamboos are highly adaptable, what makes some of these species cosmopolitan -they can be found practically anywhere on the planet (80% of bamboo forest lands and species are distributed across the Asian Pacific), including Brazil (Akinlabi et al, 2017;Bhadrawale et al, 2018;Kumar et al, 2021;Mustafa et al, 2021;Sawarkar et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Teixeira

Gonçalves

Modesto

et al. 2021

CERNE

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Background:We have developed a micropropagation technique methodology to clonal microgarden formation of Bambusa vulgaris selected adult plant. Collection site (i.e., stock plant cultivated in different environments) and seasonality of shoot collection (i.e., months of the year) effects on in vitro culture were evaluated. Explants that showed normal development were ex vitro rooted in miniincubator system, and three culture media (WPM, MS and deionized water + agar) supplemented with plant growth regulators (IBA, NAA and BAP) and antibiotic (streptomycin sulphate and culture medium free -control) were evaluated. Micropropagated plants were acclimatized in a shadow house and transferred to a semi-hydroponic system for establishment of a clonal micro-garden. The efficacy of the cloning protocol was determined with genetic fidelity analysis by ISSR molecular markers.Results: Considering all inoculations, 21.9% of nodal segments were in vitro established in nine shoot collections. The rooting percentage was 36.6%, and no interactions were observed between the use of culture medium and antibiotic. Culture medium free antibiotic resulted in 80.0% of survival and 50.0% of adventitious rooting. Micropropagated plants presented adequate growth and adaptation to ex vitro conditions in the clonal micro-garden. Molecular analyses by ISSR markers indicated the absence of genetic variations, and histological analyses revealed normal adventitious root formation originating from meristematic cells. Conclusion:The formation of a clonal micro-garden was demonstrated, proving the feasibility of the tested technique. Our results contribute to the development of a clonal propagation protocol for B. vulgaris selected adult plants.

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Section: In Vitro Establishmentsupporting

confidence: 74%

Section: In Vitro Establishmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Teixeira

Gonçalves

Modesto

et al. 2021

CERNE

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“…A solution of 0.1% mercuric chloride was used for the sterilization of field grown C. aconitifolius stem segments. Mercury chloride is a commonly used effective surface fungicide for explant sterilization [ 32 ]. Rafiq et al [ 33 ] showed that the scale of Oriental Hybrid Lilium cv.…”

Section: Discussionmentioning

confidence: 99%

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Jiang

et al. 2022

Plants

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Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. aconitifolius plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (p < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.

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“…The character of bacteria introduced to tissue cultures with plant explants can be epiphytic or endophytic. Epiphytic bacteria inhabit a surface of plants, therefore they are in the main removable by various disinfectants (Bhadrawale et al, 2018;Arab et al, 2014, Tomaszewska-Sowa andFigas, 2011;Moutia and Dookun, 1999). Bacteria living within healthy plant tissues, i.e.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Contamination of Plant in vitro Cultures in Commercial Production Detected by High-Throughput Amplicon Sequencing

Tekielska¹,

Peňázová²,

Kovács³

et al. 2019

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The study overviews results of bacterial incidence in in vitro plant tissue cultures obtained from commercial laboratory dealing with plants micropropagation. For the exploration, the 454 pyrosequencing of the 16S rRNA gene was used. Three samples of plant in vitro cultures with visual bacterial contamination were subjected for identification of present bacteria. Eleven genera as Acinetobacter, Lactobacillus, Methylobacterium, Roseomonas, Microbacterium, Mycobacterium, Curtobacterium, Acidovorax, Magnetospirillum, Chryseobacterium and Ralstonia were detected. Obtained results confirmed the advantages of high‑throughput amplicon sequencing analysis for identification of bacterial communities in contaminated plant in vitro cultures what provides an important information for applying appropriate measures to eliminate bacterial contamination.

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An improvised in vitro vegetative propagation technique for Bambusa tulda: influence of season, sterilization and hormones

Cited by 12 publications

References 15 publications

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Bacterial Contamination of Plant in vitro Cultures in Commercial Production Detected by High-Throughput Amplicon Sequencing

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