An in situ gelling system for bone regeneration of osteochondral defects

Bellini, Davide; Cencetti, Claudia; Meraner, Joachim; Stoppoloni, Daniela; d’Abusco, Anna Scotto; Matricardi, Pietro

doi:10.1016/j.eurpolymj.2015.02.043

Cited by 24 publications

(9 citation statements)

References 26 publications

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“…Their structures were reported to lead to significant rates of osteogenesis, and stimulated osteogenic differentiation of bone marrow stromal cells even in the absence of osteogenic media. This work compliments several other reports that have shown the potential of GG for bone tissue engineering and regeneration with alternative blending materials including hyaluronic acid 34 , gelatin 82 and gold nanorods 81 , and GG blending appears to provide a promising route towards bone tissue engineering…”

Section: Examples Of Engineered Tissues Using Ggsupporting

confidence: 89%

“…In most cases, GG products need to be sterilised prior to use in cell culture and TE. Various techniques are in use for this purpose including autoclaving [34][35][36] , ethylene oxide treatment 37 , microfiltration 38 , and antibiotic supplements 39 . Although no studies directly compare these methods with respect to their impacts on GG, equivalent investigations using other polysaccharides [40][41][42][43] and ECM materials 40,44,45 provide a good indication of the likely effects on GG.…”

Section: Purification and Sterilisationmentioning

confidence: 99%

“…One approach for resolving this issue is to blend GG with bioinformative materials that induce regular cell behaviour. For example, Cencetti et al, 70 Bellini et al, 34 Kang et al, 71 and Cerqueira et al 72 have each blended GG with hyaluronic acid (HA), a polysaccharide present in the mammalian ECM, to form engineered tissues for bone, intervertebral discs and vascularisation, respectively. The ECM protein fibronectin has also been employed to improve cell binding and differentiation in GG scaffolds for endothelial cell entrapment.…”

Section: Blending For Biofunctionalitymentioning

confidence: 99%

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Tissue engineering with gellan gum

et al. 2016

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Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built. Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsStevens, L. R., Gilmore, K. J., Wallace, G. Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built.

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Section: Examples Of Engineered Tissues Using Ggsupporting

confidence: 89%

Section: Purification and Sterilisationmentioning

confidence: 99%

Section: Blending For Biofunctionalitymentioning

confidence: 99%

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Tissue engineering with gellan gum

et al. 2016

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“…A biological performance was enhanced modifying GG hydrogel into new derivatives [12,13,[15][16][17][18]. There are also studies of GG hydrogel combined with other types of hydrogels such as hyaluronic acid, alginate, gelatin and polyethylene glycol (PEG) to tune the physicochemical properties [19][20][21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier

Choi

Lee

et al. 2019

Colloids and Surfaces B: Biointerfaces

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In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery. The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined. The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro. The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype. Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo. Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.

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“…Initially, a two-layered environment inside the cavity was formed; subsequently, diffusion of the formulation components promoted further hydrogel formation. The hydrogel provided a good environment for osteoblasts proliferation; the formulation had plausible adhesiveness and durability [ 28 ]. A clotrimazole-loaded formulation for anti-fungal dental application was obtained via a combination of GG as a gelling agent and Ca 2+ ions in the form of a citrate complex.…”

Section: Non-selective Ion-sensitive Formulationsmentioning

confidence: 99%

Recent Developments in Ion-Sensitive Systems for Pharmaceutical Applications

2021

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Stimuli-responsive carriers of pharmaceutical agents have been extensively researched in recent decades due to the possibility of distinctively precise targeted drug delivery. One of the potentially beneficial strategies is based on the response of the medical device to changes in the ionic environment. Fluctuations in ionic strength and ionic composition associated with pathological processes may provide triggers sufficient to induce an advantageous carrier response. This review is focused on recent developments and novel strategies in the design of ion-responsive drug delivery systems. A variety of structures i.e., polymeric matrices, lipid carriers, nucleoside constructs, and metal-organic frameworks, were included in the scope of the summary. Recently proposed strategies aim to induce different pharmaceutically beneficial effects: localized drug release in the desired manner, mucoadhesive properties, increased residence time, or diagnostic signal emission. The current state of development of ion-sensitive drug delivery systems enabled the marketing of some responsive topical formulations. Concurrently, ongoing research is focused on more selective and complex systems for different administration routes. The potential benefits in therapeutic efficacy and safety associated with the employment of multi-responsive systems will prospectively result in further research and applicable solutions.

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An in situ gelling system for bone regeneration of osteochondral defects

Cited by 24 publications

References 26 publications

Tissue engineering with gellan gum

Tissue engineering with gellan gum

Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier

Recent Developments in Ion-Sensitive Systems for Pharmaceutical Applications

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