An in vitro antagonistic efficacy validation of Rhizophora mucronata

Manilal, Aseer; Merdekios, Behailu; Idhayadhulla, Akbar; Muthukumar, C; Melkie, Mulugeta

doi:10.1016/s2222-1808(14)60622-8

Cited by 18 publications

(12 citation statements)

References 14 publications

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“…The antibacterial assay was performed as per the methodology described in our previous studies [2, 15]. Fresh cultures of respective pathogens (approx.…”

Section: Methodsmentioning

confidence: 99%

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In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

Ameya¹,

Manilal²,

Merdekios³

2017

TOMICROJ

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Background:Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria.Methods: Nicotiana tabacum L. was collected from the Western Ethiopia and extracted in seven organic solvents. An in-vitro anti-bacterial activity of plant extracts was carried out by agar well diffusion assay against microbial type culture collection of human pathogens, clinical bacterial isolates, and biofilm forming bacteria. Gas Chromatographic and Mass Spectroscopic (GC-MS) analysis was used to determine the phytochemical constituents.Results:Antimicrobial activities of plant extract vary by extraction solvents; and ethyl acetate based extracts showed better antimicrobial activities. Of the experimental organisms, biofilm forming uropathogens were the most sensitive while clinical isolates were quite resistant. Analysis of the active ethyl acetate extract by GC-MS evinced a mixture of five volatile compounds; and Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) was the major compound detected. The overall results of the present study revealed that N. tabacum L extract has high antimicrobial activities against biofilm forming uropathogens.Conclusion:High antimicrobial activity was observed in ethyl acetate extract of N. tabacum against the biofilm forming bacteria whereas the clinically isolated bacteria were the most resistant group. The antibacterial property demonstrated could be due to Pyridine, 3-(1-methyl-2-pyrrolidinyl)-(S) with a broad spectrum of activity.

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“…The antibacterial assay was performed as per the methodology described in our previous studies [2, 15]. Fresh cultures of respective pathogens (approx.…”

Section: Methodsmentioning

confidence: 99%

“…Thence, a novel class of antimicrobials is urgently needed to combat the increased incidence of multidrug resistant pathogens [2]. It is proposed that about twenty novel classes of antibiotics are required to support modern medicine during the next 50 years [3].…”

Section: Introductionmentioning

confidence: 99%

In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

Ameya¹,

Manilal²,

Merdekios³

2017

TOMICROJ

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“…vulnificus, V. alginolyticus, V. anginllarum, dan V. lohi (Manilal et al, 2010;Babuselvam et al, 2012). Selain itu, jenis mangrove ini dapat dijadikan sebagai alternatif pengobatan penyakit vibriosis pada larva lobster (Baskaran & Mohan, 2012).…”

Section: Aktivitas Antibakteri Penyebab Vibriosis Terhadap Udang Windunclassified

AKTIVITAS ANTIBAKTERI PENYEBAB VIBRIOSIS TERHADAP UDANG WINDU DARI EKSTRAK HERBAL MANGROVE Sonneratia alba DAN Bruguiera gymnorrhiza

Muliani

Tampangallo

Atmomarsono

2017

Jurnal Riset Akuakultur

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ABSTRAKPenelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak herbal mangrove Sonneratia alba dan Bruguiera gymnorrhiza terhadap penyebab penyakit vibriosis pada udang windu. Daun mangrove S. alba dan B. gymnorrhiza masing-masing diambil dari Kabupaten Maros dan Pangkep. Sampel daun dikeringanginkan selama dua minggu, ditepungkan, diekstraksi dengan metanol 80%, dan dievaporasi. Rendemen yang diperoleh dipartisi menggunakan dua jenis pelarut yaitu butanol dan dietileter. Uji bioassay dilakukan baik secara kualitatif maupun kuantitatif melalui penentuan minimum inhibition concentration (MIC) dan minimum bactericidal concentration (MBC PENDAHULUANPenyakit vibriosis atau penyakit udang berpendar merupakan salah satu jenis penyakit udang yang banyak menimbulkan kematian baik di tambak maupun di panti perbenihan. Vibriosis disebabkan oleh beberapa jenis bakteri vibrio seperti Vibrio harveyi, Vibrio parahaemolytiucus, Vibrio alginolyticus, Vibrio fischeri. Di antara beberapa jenis vibrio yang paling berbahaya adalah V. harveyi (Kannapiran et al., 2009) dan sampai saat ini penyakit ini masih merupakan kendala budidaya udang terutama di panti perbenihan.Berbagai upaya telah dilakukan untuk menanggulangi serangan penyakit pada budidaya udang, namun hingga saat ini kematian udang di tambak dan di panti perbenihan akibat serangan penyakit masih saja terus terjadi. Penggunaan bahan alam termasuk mangrove dan tanaman asosiasinya untuk penanggulangan penyakit di bidang perikanan mulai dirintis meskipun masih terbatas pada skala laboratorium di antaranya sebagai antibakteri (Velmurugan & Citarasu, 2010;Rajasekar et al., 2011;Rajeswari et al., 2012).

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“…Antimicrobial assay was performed as per the methodology described by Manilal et al, [12]. Brine shrimp cytotoxicity of the methanolic extract of various concentrations (100 to 500 µg/ml) was evaluated after Manilal et al, [13].…”

Section: Introductionmentioning

confidence: 99%

“…This assay is widely employed in the screening process of botanical for the isolation of bioactive metabolites. Cytotoxic activity of other algae from the Indian coast is already reported [13,19]. Therefore the bioassay guided purification and fractionation of P. tetrastromatica may give-forth potent antibiotic and anticancer leads.…”

Section: Introductionmentioning

confidence: 99%

An In Vitro Antibacterial and Cytotoxic Potentials of Bioactive Metabolites Extracted from Padina tetrastromatica

Manilal¹,

Mama²

2016

Transl Biomed

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Marine brown algae, Padina tetrastromatica was extracted using diverse organic solvents of increasing polarity such as diethyl ether, chloroform, acetone, dichloromethane: methanol and methanol. The obtained extracts were evaluated for antibacterial and brine shrimp cytotoxicity assays. It was found that methanolic extract of P. tetrastromatica exhibited the broadest and highest range of antibacterial activity. In cytotoxic activity, the methanolic extract of P. tetrastromatica showed significant activity (LD 50 =354.4 µg/mL). Based on the present findings, it could be inferred that the bioassay guided purification and fractionation of crude methanolic extract of P. tetrastromatica may bring forth potent novel antibiotic and anticancer leads.

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An in vitro antagonistic efficacy validation of Rhizophora mucronata

Cited by 18 publications

References 14 publications

In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

AKTIVITAS ANTIBAKTERI PENYEBAB VIBRIOSIS TERHADAP UDANG WINDU DARI EKSTRAK HERBAL MANGROVE Sonneratia alba DAN Bruguiera gymnorrhiza

An In Vitro Antibacterial and Cytotoxic Potentials of Bioactive Metabolites Extracted from Padina tetrastromatica

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