An In Vitro Evaluation of Anti-Inflammatory Activity of Newly Synthesized 1,3,4 Oxadiazole Derivatives

Abstract

In the area of heterocyclic chemistry, oxadiazole has made major contributions. An enormous number of oxadiazoles have been synthesized and exposed to biological screening; the screening results improved their status due to its potential activities and their efficacy in diverse fields of daily life. Due to its wide range of pharmacological and therapeutic effects, the 1,3,4-oxadiazole moiety is the most important oxadiazole. In this study, we discuss a new family of 2,5-disubstituted 1,3,4-oxadiazole compounds' anti-inflammatory characteristics (4a-4h).The antiinflammatory efficacy of the oxadiazole derivative was studied by utilizing Human Red Blood Cell membrane stabilization (HRBCs) method. Many synthesized compounds displayed remarkable anti-inflammatory activity in HRBCs test. In the novel synthesized derivatives, compounds 2-(5-bromo-2-(trifluoromethoxy)phenyl)-5-p-tolyl1,3,4-oxadiazole (4h) and 2-(5-bromo-2-(trifluoromethoxy) phenyl)-5-phenyl-1,3,4-oxadiazole (4a) exhibited the maximum activity of 90.76 % and 88.35 % protection, respectively at concentration 500 µg/ml, compared with DFS that showed 91.86 % inhibition of RBC haemolysis at the same concentration.