An In Vitro Evaluation of the Growth of Human Periodontal Ligament Fibroblasts after Exposure to a Methacrylate-based Endodontic Sealer

Heitman, Erich P.; Joyce, Anthony P.; McPherson, James C.; Roberts, Susan Jo; Chuang, Augustine

doi:10.1016/j.joen.2007.10.018

Cited by 24 publications

(35 citation statements)

References 27 publications

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“…Physicochemical and biological properties of a novel PU sealer A high degree of NCO group conversion could avoid the cytotoxicity 26,27 caused by residual free monomers during polymerization. Increasing the degree of conversion not only ensures the elimination of uncured monomers, which may cause adverse biological reactions, but it also results in more favorable mechanical properties, such as surface hardness, flexural strength, and fracture toughness.…”

mentioning

confidence: 99%

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Wang¹,

Zuo²,

Zhao³

et al. 2015

IJN

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A root canal sealer with antibacterial activity can be efficacious in preventing reinfection that results from residual microorganisms and/or the leakage of microorganisms. In the present study, a series of injectable, self-curing polyurethane (PU)-based antibacterial sealers with different concentrations of silver phosphate (Ag 3 PO 4 ) were fabricated. Subsequently, their physicochemical properties, antibacterial abilities, and preliminary cytocompatibilities were evaluated. The results indicated that the fabricated PU-based sealers can achieve a high conversion rate in a short amount of time. More than 95% of the isocyanate group of PU sealers with 3 wt% (PU3) and 5 wt% (PU5) concentrations of Ag 3 PO 4 were included in the curing reaction after 7 hours. With the exception of those for film thickness for PU5, the results of setting time, film thickness, and solubility were able to meet the requirements of the International Organization for Standardization. The antibacterial tests showed that PU3 and PU5 exhibit stronger antimicrobial effects than that achieved with 1 wt% Ag 3 PO 4 (PU1) and AH Plus (positive control) against Streptococcus mutans . The cytocompatibility evaluation revealed that the PU1 and PU3 sealers possess good cytocompatibility and low cytotoxicity. These results demonstrate that the PU3 sealer offers good physicochemical and antimicrobial properties along with cytocompatibility, which may hold great application potential in the field of root canal fillings.

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mentioning

confidence: 99%

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Wang¹,

Zuo²,

Zhao³

et al. 2015

IJN

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“…Furthermore, the Epiphany and Resilon systems induced a slight inflammatory reaction after 42 days. The toxicity of Epiphany might be explained by the presence of unpolymerized hydrophilic monomers that can readily diffuse into the cell culture medium 32) and elicit a significant toxicity 33) . The second generation of Epiphany (Epiphany SE) is a non-etching methacrylate with an acidic resin monomer that is originally found in primers that have been incorporated into self-adhesive resin-based sealers 34) .…”

Section: Discussionmentioning

confidence: 99%

“…First-generation Epiphany was shown to induce bone formation and cause a slight osseous inflammatory reaction in the in vivo experiment 8) . The in vitro experiments demonstrated that Epiphany in freshly mixed or set conditions showed severe-to-moderate cytotoxicity 9) , and its cytotoxicity increased with time, posing significant risks 10,11) . The other in vitro studies tested the effect of Epiphany on human fibroblasts and L929 cells, and showed its strong cytotoxicity on these cells 12,13) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the biocompatibility of resin-based root canal sealers in rat periapical tissue

et al. 2013

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We evaluated the biocompatibility of resin-based root canal sealers (RCSs) in the periapical tissues of rats. Wistar rats underwent tooth replantation for reproducing the response of periapical tissue with RCSs. The resin-based Epipany SE™, AH Plus Jet™, the eugenol-based sealer (Canals) and a control group were employed. The upper right first molar was extracted and applied with RCSs on apices, and then the tooth was repositioned. Histological evaluation demonstrated that mild inflammation occurred in the periapical tissue with Epiphany and AH Plus Jet sealers on day 7, whereas Canals induced severe-to-moderate inflammation. The statistical analyses demonstrated that the significant differences were observed between Canals and the other groups on day 7 regarding inflammatory response. On day 14, the lesions induced by all sealers were healed and replaced predominantly by fibrous connective tissue. Our results suggest that Epiphany SE and AH Plus Jet are good biocompatible materials.

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“…36,98 Epiphany sealer has been shown to be cytotoxic when compared to zinc oxide eugenol and epoxy-resin based sealers 100,105-110 with one study suggesting that it became more cytotoxic with increased exposure time. 111 However, it has also been demonstrated that although Epiphany was cytotoxic it did not effect the viability of human leucocytes. 112 The reported cytotoxicity may be due to the leakage of uncured monomer from the oxygen inhibition layer, 100,101,113,114 or due to the degradation of the sealer in an aqueous environment.…”

Section: Biocompatibilitymentioning

confidence: 99%

Root canal filling using Resilon: a review

Shanahan

Duncan

2011

Br Dent J

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An In Vitro Evaluation of the Growth of Human Periodontal Ligament Fibroblasts after Exposure to a Methacrylate-based Endodontic Sealer

Cited by 24 publications

References 27 publications

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Evaluation of the biocompatibility of resin-based root canal sealers in rat periapical tissue

Root canal filling using Resilon: a review

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