An in vitro radiation induced mutagenesis-selection system for salinity tolerance in sugarcane

Patade, Vikas Yadav; Suprasanna, Penna

doi:10.1007/s12355-009-0042-4

Cited by 32 publications

(10 citation statements)

References 24 publications

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“…Proline accumulation is a common physiological response in numerous plants to various collections of abiotic and biotic stresses (Alhasnawi et al, 2014a). Therefore, the present results reflective of proline accumulation, in line with those of Gandonou et al, (2006); Patade and Suprasanna, (2009), suggests a higher accumulation of proline in callus exposed to salinity stress medium. Under salinity conditions, growth performance and salt tolerance of common rice callus with regards to fresh weight, dry weight, and RGR were effectively improved with β-glucan supplementation.…”

Section: Biochemical Estimationsupporting

confidence: 87%

Accumulation of antioxidants in rice callus (Oryza sativa L.) induced by β-glucan and salt stress

Alhasnawi¹,

Zain²,

Kadhimi³

et al. 2017

Aust J Crop Sci

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Salinity stress causes a considerable reduction in callus growth. Mushroom polysaccharides (β-glucan) are the most promising groups of antioxidant compounds. An in vitro experiment was conducted to evaluate the potential function of exogenously applied β-glucan in alleviating the accumulation of antioxidants in rice callus. In this study, morphological, chemical, and biochemical parameters of an embryogenic callus of the rice variety, MR269, were investigated under 200mM NaCl stress conditions after pre-treatment with (0, 0.5, 1 and 1.5 mg/L) β-glucan in culture media. The present study sought to evaluate chemical, biochemical, and callus growth characterization. The results revealed that callus exposed to stressful media exhibited a significant decrease in callus growth and contents of K + and Ca +2. In addition, significant accumulation of Na + and Na + /K + was found, as well as an enhanced enzymatic antioxidant system and elevated proline activities under NaCl conditions. Furthermore, exogenous addition of β-glucan at 0.5-1.5mg/L under NaCl stress induced a pronounced, significant increase of callus growth, K + and Ca +2 contents, and enzymatic antioxidant and proline activities. In contrast, a significant decrease was found in the levels of Na + and Na + /K + , particularly in callus treated with NaCl. β-Glucan ameliorates the adverse effects of NaCl stress, and the extent of amelioration depends on the type of β-glucan agent as well as treatment duration (three months). The changes mentioned are important for determining the morphological, chemical, and biochemical parameters of salinity tolerance in callus. This study demonstrated that exogenous β-glucan exhibits alleviation of the harmful effects of salt stress and increases salinity resistance in callus rice.

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Section: Biochemical Estimationsupporting

confidence: 87%

Accumulation of antioxidants in rice callus (Oryza sativa L.) induced by β-glucan and salt stress

Alhasnawi¹,

Zain²,

Kadhimi³

et al. 2017

Aust J Crop Sci

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“…Culture exposed to 100 mM treatment almost 50% survivals as compared to control nontreated culture (Table 2). Similar results obtained by Patade and Suprasanna (2009) for 171.1 mM and Munir and Aftab (2009) for 120 mM concentration of NaCl. These results are in close agreement with those reported by Mallikarjun et al 2008; Shomeili et al 2011; Gandonou et al (2005b).…”

Section: In Vitro Salinity Screeningsupporting

confidence: 82%

Evaluation of Differential Physiological and Biochemical Response of Sugarcane (Saccharum spp. Complex cv. Co 94012) Parent and Mutant’s in Response to Salt Stress

Gadakh¹,

Patel²,

Ban³

2017

IJBSM

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The present investigation carried out at Main Sugarcane Research Station, Navsari Agricultural University, Navsari during 2011−2014 to develop salt tolerance lines in sugarcane. Sugarcane mutants against salt tolerance were derived using in vitro mutagenesis and regeneration under NaCl stress. Sugarcane cv. 94012 was used for callus development from meristimatic leaf whorl on MS medium supplemented with 4 mg/l 2,4-D+2% sucrose. At treatment of Ethyl Methane Sulphonate (0.5%) for 2 hours treatment 48.60% calli survived similarly out of four concentration of NaCl 100 mM concentration of NaCl give at least 50.20% survived of calli. So these concentrations were used for development of salt tolerant mutant in sugarcane. The calli were treated with EMS lethal dose (LD 50) placed on shoot regeneration media containing LD 50 concentration of NaCl. The plants which regenerated from the tolerant calli were grown in pot culture system under five levels of salinity stress as compared to parental plants (source variety). With increasing supply of NaCl, root growth was more adversely affected. The growth of shoot, Chlorophyll content, photosynthesis rate, stomatal conductance and dry matter showed a decreasing trend but it shows significantly slow rate in tolerant mutants than parental plants. Mutant plants performed significantly well than parental plants in salt stress condition in all physiological and biochemical parameters. The result showed that high salt concentration Na + content in shoot of tolerant mutant and but was less than the normal plant. While, K + content in shoot was high in tolerant mutants than normal plants.

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“…The application of radiation-induced mutagenesis with in vitro culture has proved effective in the induction of genetic variation, selection, and multiplication of mutant clones [6,9,27]. Assessment of radiosensitivity is a prerequisite for identifying a suitable dose for in vitro mutagenesis of a particular cultivar.…”

Section: Discussionmentioning

confidence: 99%

Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane (Saccharum officinarum L.)

et al. 2015

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Gamma ray-induced in vitro mutagenesis and selection for salt (NaCl) tolerance were investigated in sugarcane (Saccharum officinarum L.). Embryogenic callus cultures were irradiated (10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaCl (0, 50, 100, 150, 200, and 250 mmol L −1 ). Increasing NaCl concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na + and K + concentration. Higher accumulation of proline and glycine betaine was observed in NaCl stressed callus irradiated at 20 Gy. Na + concentration increased and K + concentration decreased with increasing salt level. Irradiated callus showed 50-60% regeneration under NaCl stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80-85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%, number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane.

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An in vitro radiation induced mutagenesis-selection system for salinity tolerance in sugarcane

Cited by 32 publications

References 24 publications

Accumulation of antioxidants in rice callus (Oryza sativa L.) induced by β-glucan and salt stress

Accumulation of antioxidants in rice callus (Oryza sativa L.) induced by β-glucan and salt stress

Evaluation of Differential Physiological and Biochemical Response of Sugarcane (Saccharum spp. Complex cv. Co 94012) Parent and Mutant’s in Response to Salt Stress

Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane (Saccharum officinarum L.)

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