An In Vitro Study on the Effect of Amorphous Calcium Phosphate and Fluoride Solutions on Color Improvement of White Spot Lesions

Abstract

The ability of remineralizing agents to improve the color of white spot lesions (WSL) is an important aspect that should be investigated. The aim of this study was to evaluate the effects of 0.05% amorphous calcium phosphate (ACP), 0.5% ACP, and 0.05% fluoride solutions, as well as artificial saliva on the color improvement of white spot lesions (WSLs). In this in vitro study, 50 human premolar teeth were randomly classified into five groups. At baseline, all the samples were assessed by using a colorimeter (E0). Then, white spot lesions were induced on the surface of the teeth by means of a pH-cycling model, and the colorimeter was used again (E1). Afterwards, samples of the 1st and 2nd groups were kept in 0.05% ACP and 0.5% ACP solutions for 1 min/day, respectively. The 3rd group specimens were placed in 0.05% fluoride solution for 1 min/day. The other two groups were kept in artificial saliva and distilled in water separately. All the samples were assessed by the colorimeter for a third time (E2). We found no significant difference between the groups in ∆E1. There was also no significant difference among 0.05% ACP solution, 0.5% ACP solution, 0.05% fluoride solution, and artificial saliva considering ∆E2. However, a significant difference was noted between the above-mentioned solutions and distilled water in ∆E2. With respect to ∆E3, there were considerable differences between ACP solution and artificial saliva. The same results were obtained for the difference between fluoride solution and artificial saliva. However, no significant difference was found among 0.05% ACP, 0.5% ACP, and 0.05% fluoride solutions in terms of ∆E3. In Conclusion, ACP is as effective as fluoride in the color improvement of WSLs and the recommended treatment for this purpose is daily use of 0.05% ACP, 0.5% ACP or 0.05% fluoride solutions.

In vitro remineralization of enamel white spot lesions with a carrier-based amorphous calcium phosphate delivery system

“…26 The L value reveals lightness, a value represents the position of red or green axes, and b value demonstrates the position of yellow or blue axes. 27 The color measurement system was made up of a spectrophotometer (PR-650 Spectra Scan, equipped with MS-75 and SL-0.5X lens;…”

Clinical Evaluation of Different Minimal Invasive Treatment Modalities of Mild to Moderate Dental Fluorosis Using A Visual Analog Scale.

“…et al, who found that no significant difference in color improvement of white spot lesions between sodium fluoride and ACP. 15 This conflict might have resulted from application of ACP as mouthwash lacking casein, so the reaction was undermined by the rapid formation of calcium phosphate phase rather than diffusion into subsurface lesion when stabilized by casein.…”

Effect of ibuprofen and low-intensity pulsed ultrasound on the reduction of pain after initial archwire placement: a double-blind randomized clinical trial

“…Like any medical treatment, orthodontic treatment may have side effects such pain, root resorption, periodontal disease, temporomandibular dysfunction, caries, speech problems and enamel damage. [1][2][3][4][5] Pain is a common experience in orthodontic patients and is considered the most important factor deterring patient referral for further treatment. 6 Orthodontic pain arises from ischemia, inflammation, and edema in the compressed periodontal ligament.…”