An in vivo microfabricated scaffold for tendon repair

Curtis, Adam; Wilkinson, C. D. W.; Crossan, J. F.; Broadley, Caroline; Darmani, Homa; Johal, K K; Jörgensen, H; Monaghan, W.

doi:10.22203/ecm.v009a07

Cited by 26 publications

(18 citation statements)

References 13 publications

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“…122,123,132 The topology-related alignment and guidance has proven to be useful in vivo, for implantable scaffolds have been molded with nanogrooves for improved tendon repair. 33 Additionally, adrenal gland cells were grown on nanogrooves, 50 which are commonly used cell models to study the repair of the nervous system because they exhibit neuronal characteristics in the presence of nerve growth factor (NGF). To investigate whether physical topology also affects neurite outgrowth, cells exposed to suboptimal concentrations of NGF extended more neurites on nanogrooves than on flat substrates.…”

Section: Nanotopologymentioning

confidence: 99%

Nanotechnology for Cell–Substrate Interactions

et al. 2006

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In the pursuit to understand the interaction between cells and their underlying substrates, the life sciences are beginning to incorporate micro- and nanotechnology-based tools to probe and measure cells. The development of these tools portends endless possibilities for new insights into the fundamental relationships between cells and their surrounding microenvironment that underlie the physiology of human tissue. Here, we review techniques and tools that have been used to study how a cell responds to the physical factors in its environment. We also discuss unanswered questions that could be addressed by these approaches to better elucidate the molecular processes and mechanical forces that dominate the interactions between cells and their physical scaffolds.

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Section: Nanotopologymentioning

confidence: 99%

Nanotechnology for Cell–Substrate Interactions

et al. 2006

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“…This phenomenon is termed topographic or contact guidance [Dunn and Heath, 1976;Brunette, 1986a;Clark et al, 1987Clark et al, , 1990Curtis and Wilkinson, 1998] and is exhibited by a variety of cell populations contacting grooved substrata of various chemical compositions, fabricated by different techniques and having different groove/ridge geometries and dimensions [Brunette et al, 1983;Chehroudi et al, 1988;Clark et al, 1991;den Braber et al, 1995den Braber et al, , 1996Walboomers et al, 1998;Miller et al, 2001;Dalby et al, 2003;Curtis et al, 2005;Evans et al, 2005].…”

Section: Introductionmentioning

confidence: 99%

Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Hamilton

Oakley

Jaeger

et al. 2009

Cell Motil. Cytoskeleton

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Anisotropic substrata such as micromachined grooves can control cell shape, orientation, and the direction of cell movement, a phenomena termed topographic guidance. Although many types of cells exhibit topographic guidance, little is known regarding cell responses to conflicting topographic cues. We employed a substratum with intersecting grooves in order to present fibroblasts and epithelial cells with conflicting topographic cues. Using time-lapse and confocal microscopy, we examined cell behavior at groove intersections. Migrating fibroblasts and epithelial cells typically extended a cell process into the intersection ahead of the cell body. After travelling along the "X" groove to enter the intersection, the leading lamellipodia of the cell body encountered the perpendicular "Y" groove, and spread latterly along the "Y" groove. The formation of lateral lamellipodia resulted in cells forming "T" or "L" morphologies, which were characterized by the formation of phosphotyrosine-rich focal adhesions at the leading edges. The "Y" groove did not prove an absolute barrier to cell migration, particularly for epithelial cells. Analysis of cytoskeletal distribution revealed that F-actin bundles did not adapt closely to the groove patterns, but typically did align to either the "X" or "Y" grooves. In contrast microtubules (MT) adapted closely to the walls. Inhibition of microtubule nucleation attenuated fibroblast and epithelial cell orientation within the intersection of the perpendicular grooves. We conclude that MT may be the prime determinant of fibroblast and epithelial cell conformation to conflicting topographies.

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“…Three-dimensional (3D) cell cultures have a wide variety of applications in basic cell and tissue studies (Yin et al, 2004;Entcheva and Bien, 2003;Motlagh et al, 2003), in vivo tissue repair and clinical practice (Curtis et al, 2005;Stoklosowa, 2001). Previous studies have attempted to provide grooved surfaces for cardiomyocytes to mimic their in vivo 3D circumstances, and have shown that the alignment, orientation and shape of the cells were guided by the grooved surfaces (Yin et al, 2004;Entcheva and Bien, 2003;Motlagh et al, 2003).…”

Section: Introductionmentioning

confidence: 99%