An increase in switching frequency correlates with an increase in recombination of the ribosomal chromosomes of Candida albicans strain 3153A

Ramsey, H. E.; Morrow, Brian J.; Soll, David R.

doi:10.1099/13500872-140-7-1525

Cited by 38 publications

(44 citation statements)

References 21 publications

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“…However, the acquisition of phenotypic traits in tandem with chromosomal variation has been reported previously in C. albicans and C. glabrata. Chromosomal rearrangements and deletions have been associated with phenotypic switching in C. albicans (43). Moreover, the fluconazole resistance phenotype of a C. glabrata isolate has been linked to the duplication of the chromosome carrying the ERG11 gene, encoding the fluconazole target enzyme (27).…”

Section: Microevolution In Vivomentioning

confidence: 99%

Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo

et al. 2002

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The present study investigates further the population structure of Candida dubliniensis and its ability to exhibit microevolution. Using 98 isolates (including 80 oral isolates) from 94 patients in 15 countries, we confirmed the existence of two distinct populations within the species C. dubliniensis, designated Cd25 group I and Cd25 group II, respectively, on the basis of DNA fingerprints generated with the C. dubliniensis-specific probe Cd25. The majority of Cd25 group I isolates (48 of 71, 67.6%) were from human immunodeficiency virus (HIV)-infected individuals, whereas the majority of Cd25 group II isolates (19 of 27, 70.4%) were from HIV-negative individuals (P < 0.001). Nucleotide sequence analysis of the internal transcribed spacer (ITS) regions of the rRNA genes from 19 representative isolates revealed the presence of four separate genotypes. All of the Cd25 group I isolates tested belonged to genotype 1, while the Cd25 group II population was comprised of three distinct genotypes (genotypes 2 to 4), which corresponded to distinct clades within the Cd25 group II population. These findings were confirmed using genotype-specific PCR primers with 70 isolates. We also showed that C. dubliniensis can exhibit microevolution in vivo and in vitro as occurs in other yeast species. DNA fingerprinting using the C. dubliniensis probes Cd25, Cd24, and Cd1 and karyotype analysis of multiple oral isolates recovered from the same specimen from each of eight separate patients revealed microevolution in six of eight of the clonal populations. Similarly, sequential clonal isolates from various anatomical sites in two separate patients exhibited microevolution. Microevolution was also shown to occur when two clinical isolates susceptible to fluconazole were exposed to the drug in vitro. The epidemiological significance of the four C. dubliniensis genotypes and the ability of C. dubliniensis to undergo microevolution has yet to be established.

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Section: Microevolution In Vivomentioning

confidence: 99%

Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo

et al. 2002

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“…The karyotype variation among the clinical isolates seems to confer one kind of genetic variation in this organism. Several groups have reported a correlation between the diversity of karyotype and colony morphology (Suzuki et al, 1989;Rustchenko et al, 1990;Rustchenko, 1991;Ramsey et al, 1994). Rustchenko et al (1994) showed a correlation between a karyotypic change and the assimilation of carbon source in L-sorbose and D-arabinosepositive mutants derived from a laboratory strain 3153A of C. albicans.…”

Section: Introductionmentioning

confidence: 99%

Chromosome translocation induced by the insertion of the URA blaster into the major repeat sequence (MRS) in Candida albicans

Iwaguchi

Suzuki

Sakai

et al. 2004

Yeast

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Electrophoretic karyotype studies have shown that clinical isolates of Candida albicans have extensive chromosome length polymorphisms. Chromosome translocation is one of the causes of karyotypic variation. Chromosome translocation events have been shown to occur very frequently at or near the major repeat sequence (MRS) on chromosomes. The MRS consists of the repeated sequences RB2, RPS and HOK, and the repeated sequences are considered to be the template for recombination. To investigate which element of the MRS is important for chromosome translocation, we constructed three cassettes, each containing a URA blaster and sequences homologous to one of the repeats, for insertion into the MRS region on the chromosomes. The ura3 strain STN22u2, which shows a stable, standard karyotype, was transformed with each construct. Insertion events with each cassette occurred at almost all chromosomes. Insertion into the RB2 repeat, but not into the RPS repeat, was accompanied by chromosome translocation in some transformants: chromosome translocations between chromosomes R and 7 and chromosomes 1 and 7 were found, as well as deletions of 7A and 7C from chromosome 7. We conclude that the insertion at the RB2 region may initiate chromosome translocation in C. albicans.

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“…Suzuki et al (1989) and Rustchenko-Bulgac et al (1990) proposed that there was a correlation between the diversity of karyotype and diversity of phenotype. Several reports comparing karyotypic change and phenotypes-such as the ability to switch (Ramsey et al 1994), assimilation of carbon source (Rustchenko et al 1994 ), and resistance to the antifungal drug fluconazole (Mori et al 1998)-have been published. Despite great effort, no correlation between the diversity of karyotype and diversity of phenotype was shown until Rustchenko and coworkers found an association between the loss or partial deletion of one of the homologs of chromosome 5 and assimilation of sorbose (Janbon et al 1998).…”

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confidence: 99%

Fine-Resolution Physical Mapping of Genomic Diversity in Candida albicans

Chibana¹,

Beckerman²,

Magee³

2000

Genome Res.

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It has been suggested that Candida albicans, a diploid asexual fungus, achieves genetic diversity by genomic rearrangement. This important human pathogen may provide a system in which to analyze alternate routes to genomic diversity. C. albicans has a highly variable karyotype; its chromosomes contain a middle repeated DNA sequence called the Major Repeat Sequence (MRS), composed of subrepeats HOK, RPS, and RB2. RPS is tandemly repeated while the other subrepeats occur once in each MRS. Chromosome 7, the smallest of the eight chromosomes, has been previously mapped. The complete physical map of this chromosome was used to analyze chromosome 7 diversity in six strains, including two well-characterized laboratory strains (1006 and WO-1) and four clinical ones. We found four types of events to explain the genomic diversity: 1) Chromosome length polymorphism (CLP) results from expansion and contraction of the RPS; 2) reciprocal translocation occurs at the MRS loci; 3) chromosomal deletion; and (4) trisomy of individual chromosomes. These four phenomena play an important role in generating genomic diversity in C. albicans.

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An increase in switching frequency correlates with an increase in recombination of the ribosomal chromosomes of Candida albicans strain 3153A

Abstract: When cells of

Cited by 38 publications

References 21 publications

Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo

Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo

Chromosome translocation induced by the insertion of the URA blaster into the major repeat sequence (MRS) in Candida albicans

Fine-Resolution Physical Mapping of Genomic Diversity in Candida albicans

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