An increased proportion of progesterone receptor A in peripheral B cells from women who ultimately underwent spontaneous preterm birth

Campe, Kim-Norina Jutta; Redlich, Anke; Zenclussen, Ana Claudia; Busse, Mandy

doi:10.1016/j.jri.2022.103756

Cited by 6 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, we have shown that B cells play a role in the pathophysiology of PTB. B cells upregulated molecules involved in the pattern recognition and antigen presentation and exhibited a transition from the immune regulatory towards inflammatory phenotypes in patients with PTB [12][13][14]22]. Previous research suggests that B1 cells and plasmablasts in amniotic fluid, which have both the capacity to produce IL-10, were affected in women experiencing PTB and chronic chorioamnionitis, but not in cases of preterm prelabor rupture of membranes [23,24].…”

Section: Discussionmentioning

confidence: 99%

B Cells Induce Early-Onset Maternal Inflammation to Protect against LPS-Induced Fetal Rejection

Uehre,

Tchaikovski,

Ignatov

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

The maternal balance between B regulatory (Breg) cells and inflammatory B cells is of central importance for protection against preterm birth (PTB). However, the impact of B cell signaling in early maternal and fetal immune responses on inflammatory insults remains underinvestigated. To understand which role B cells and B-cell-specific signaling play in the pathogenesis of PTB, the later was induced by an injection of LPS in B cell-sufficient WT mice, CD19−/−, BMyD88−/− and µMT murine dams at gestational day 16 (gd 16). WT dams developed a strong inflammatory response in their peritoneal cavity (PC), with an increased infiltration of granulocytes and enhanced IL-6, TNF-α, IL-17 and MCP-1 levels. However, they demonstrated a reduced NOS2 expression of PC macrophages 4 h after the LPS injection. Simultaneously, LPS-challenged WT dams upregulated pregnancy-protective factors like IL-10 and TARC. The concentrations of inflammatory mediators in the placental supernatants, amniotic fluids, fetal serums and gestational tissues were lower in LPS-challenged WT dams compared to CD19−/−, BMyD88−/− and µMT dams, thereby protecting WT fetuses from being born preterm. B cell deficiency, or the loss of B-cell-specific CD19 or MyD88 expression, resulted in an early shift from immune regulation towards inflammation at the fetomaternal interface and fetuses, resulting in PTB.

show abstract

Section: Discussionmentioning

confidence: 99%

B Cells Induce Early-Onset Maternal Inflammation to Protect against LPS-Induced Fetal Rejection

Uehre,

Tchaikovski,

Ignatov

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although nuclear PRs are expressed in a limited number of cancer cell lineages, membrane PRs are widely expressed in many cancer cell lines [ 26 ]. B cells have also been reported to express PRs [ 27 , 28 ]; however, enhanced expression of PRs has not been reported in B cells or B-lineage cancer cell lines. Therefore, in B-lineage cancer cells, unidentified receptors may be expressed and involved in this function.…”

Section: Discussionmentioning

confidence: 99%

Liposome-encapsulated progesterone efficiently suppresses B-lineage cell proliferation

Seki,

Suzuki,

Ohshima

et al. 2024

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

“…Reduced progesterone levels in these women may lead to an increased expression of their receptors. This can further provoke inflammation, lead to a disruption of the mother's tolerance for the fetus, and induce labor [56]. As indicated, to maintain a pregnancy, progesterone or dydrogesterone, probably through PIBF, reduce the production of IL-6 and prevent preterm birth [57].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Progestins on Cytokine Production in the Peripheral Blood Mononuclear Cells of Menopausal Women and Their Luminol-Dependent Chemiluminescence

Pavlik,

Shimanovsky,

Zemlyanaya

et al. 2023

Molecules

View full text Add to dashboard Cite

Steroid hormones are the key regulators of inflammatory and autoimmune processes. The role of steroid hormones is mostly inhibitory in these processes. The expression of IL-6, TNFα, and IL-1β, as markers of inflammation, and TGFβ, as a marker of fibrosis, could be useful tools to predict the response of an individual’s immune system to the different progestins suitable for the treatment of menopausal inflammatory disorders, including endometriosis. In this study, the progestins P4 and MPA, as well as the novel progestin gestobutanoyl (GB), which possess potent anti-inflammatory properties towards endometriosis, were studied at a fixed concentration of 10 µM. Their influence on the production of the above cytokines in PHA-stimulated peripheral blood mononuclear cells (PBMCs) during 24 h incubation was evaluated by ELISA. It was found that synthetic progestins stimulated the production of IL-1β, IL-6, and TNFα and inhibited TGFβ production, while P4 inhibited IL-6 (33% inhibition) and did not influence TGFβ production. In the MTT-viability test, P4 also decreased PHA-stimulated PBMC viability by 28% during 24 h incubation, but MPA and GB did not have any inhibitory or stimulatory effects. The luminol-dependent chemiluminescence (LDC) assay revealed the anti-inflammatory and antioxidant properties of all the tested progestins, as well as some other steroid hormones and their antagonists: cortisol, dexamethasone, testosterone, estradiol, cyproterone, and tamoxifen. Of these, tamoxifen showed the most pronounced effect on the oxidation capacity of PBMC but not on that of dexamethasone, as was expected. Collectively, these data demonstrate that PBMCs from menopausal women respond differently to P4 and synthetic progestins, most likely due to distinct actions via various steroid receptors. It is not only the progestin affinity to nuclear progesterone receptors (PR), androgen receptors, glucocorticoid receptors, or estrogen receptors that is important for the immune response, but also the membrane PR or other nongenomic structures in immune cells.

show abstract

An increased proportion of progesterone receptor A in peripheral B cells from women who ultimately underwent spontaneous preterm birth

Cited by 6 publications

References 43 publications

B Cells Induce Early-Onset Maternal Inflammation to Protect against LPS-Induced Fetal Rejection

B Cells Induce Early-Onset Maternal Inflammation to Protect against LPS-Induced Fetal Rejection

Liposome-encapsulated progesterone efficiently suppresses B-lineage cell proliferation

The Effect of Progestins on Cytokine Production in the Peripheral Blood Mononuclear Cells of Menopausal Women and Their Luminol-Dependent Chemiluminescence

Contact Info

Product

Resources

About