An indirect competitive assay-based aptasensor for detection of oxytetracycline in milk

Kim, Chong-Han; Lee, Lyun-Pyo; Min, Jeong-Ran; Lim, Myung-Woon; Jeong, Sang‐Hee

doi:10.1016/j.bios.2013.08.003

Cited by 111 publications

(41 citation statements)

References 29 publications

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“…The individual detection of each member is also possible using specific recognition reagents such as aptamers with the promise of a more rapid detection. However, methods developed so far, a voltammetric aptasensor [57] and a conventional competitive assay on microtiter plates [58], still require a rather complex pretreatment of the milk samples. A limited degree of cross-reactivity with other tetracycline members was found in both cases.…”

Section: Other Growing Food Concernsmentioning

confidence: 99%

Aptamer-Based Analysis: A Promising Alternative for Food Safety Control

Amaya-González

de‐los‐Santos‐Álvarez

Miranda‐Ordieres

et al. 2013

Sensors

123

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Ensuring food safety is nowadays a top priority of authorities and professional players in the food supply chain. One of the key challenges to determine the safety of food and guarantee a high level of consumer protection is the availability of fast, sensitive and reliable analytical methods to identify specific hazards associated to food before they become a health problem. The limitations of existing methods have encouraged the development of new technologies, among them biosensors. Success in biosensor design depends largely on the development of novel receptors with enhanced affinity to the target, while being stable and economical. Aptamers fulfill these characteristics, and thus have surfaced as promising alternatives to natural receptors. This Review describes analytical strategies developed so far using aptamers for the control of pathogens, allergens, adulterants, toxins and other forbidden contaminants to ensure food safety. The main progresses to date are presented, highlighting potential prospects for the future.

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Section: Other Growing Food Concernsmentioning

confidence: 99%

Aptamer-Based Analysis: A Promising Alternative for Food Safety Control

Amaya-González

de‐los‐Santos‐Álvarez

Miranda‐Ordieres

et al. 2013

Sensors

123

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“…16–20 Compared to other probes, aptamers generated from in vitro selection have many advantages, including high affinity and excellent specificity. 21–25 However, several major limitations have impeded widespread adoption of this method for exosome detection. Currently, for instance, aptamers developed for targeting exosomes are very rare, which, in part, can be attributed to the lack of efficient aptamer selection methods.…”

mentioning

confidence: 99%

Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes

et al. 2017

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Exosomes are extracellular vesicles (50–100 nm) circulating in biofluids as intercellular signal transmitters. Although the potential of cancerous exosomes as tumor biomarkers is promising, sensitive and rapid detection of exosomes remains challenging. Herein, we combined the strengths of advanced aptamer technology, DNA-based nanostructure, and portable electrochemical device to develop a nanotetrahedron (NTH)-assisted aptasensor for direct capture and detection of hepatocellular exosomes. The oriented immobilization of aptamers significantly improved the accessibility of an artificial nucleobase-containing aptamer to suspended exosomes, and the NTH-assisted aptasensor could detect exosomes with 100-fold higher sensitivity when compared to the single-stranded aptamer-functionalized aptasensor. The present study provides a proof-of-concept for sensitive and efficient quantification of tumor-derived exosomes. We thus expect the NTH-assisted electrochemical aptasensor to become a powerful tool for comprehensive exosome studies.

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“…The aptasensor showed higher selectivity for TET than the other structural analogs of OTC and DOX [99]. Recently, using ssDNA aptamers, Kim et al [100] developed an indirect competitive assay-based aptasensor for detection of OTC in milk, with the limit of detection 49.8 μg/L and recovery rate of more than 90 %. Han et al [101] identified specific and high-affinity RNA aptamers with 2′-fluoro-2′-deoxyribonucleotide modified pyrimidine nucleotides bound to danofloxacin, but not to TET, which could be used for the rapid and cost-effective detection and sensing of danofloxacin in foods or food products.…”

Section: Antibioticsmentioning

confidence: 99%

Aptamer-Based Technology for Food Analysis

Liu

Zhang

2014

Appl Biochem Biotechnol

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Aptamers are short and functional single-stranded oligonucleotide sequences selected from systematic evolution of ligands by exponential enrichment (SELEX) process, which have the capacity to recognize various classes of target molecules with high affinity and specificity. Various analytical aptamers acquired by SELEX are widely used in many research fields, such as medicine, biology, and chemistry. However, the application of this innovative and emerging technology to food safety is just in infant stage. Food safety plays a very important role in our daily lives because varieties of poisonous and harmful substances in food affect human health. Aptamer technique is promising, which can overcome many disadvantages of existing detection methods in food safety, such as long detection time, low sensitivity, difficult, and expensive antibody preparation. This review provides an overview of various aptamer screening technologies and summarizes the recent applications of aptamers in food safety, and future prospects are also discussed.

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An indirect competitive assay-based aptasensor for detection of oxytetracycline in milk

Cited by 111 publications

References 29 publications

Aptamer-Based Analysis: A Promising Alternative for Food Safety Control

Aptamer-Based Analysis: A Promising Alternative for Food Safety Control

Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes

Aptamer-Based Technology for Food Analysis

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