An Inductively Coupled Plasma Carbon Emission Detector for Aqueous Carbohydrate Separations by Liquid Chromatography

Abstract

An inductively coupled plasma atomic emission spectrometer is used to detect carbon-containing compounds following separation by high-performance liquid chromatography. A calcium form ligand exchange column with distilled and deionized water as the mobile phase is used to separate carbohydrates. The eluting species are detected by monitoring the carbon atomic emission line at 193.09 nm. The mass detection limits using a photomultiplier tube for sucrose and glucose are 50 ng, while that for fructose is 60 ng. The carbon emission detector should provide the same detection limit for any compound with a similar mass percent of carbon, whether or not the compound exhibits appreciable absorption characteristics. While the carbon emission detector will universally detect any organic compound, it will discriminate against species with high molar absorptivity that may be present at low concentration. Such species may act as interferences in chromatograms generated with conventional UV-visible absorption detectors. To demonstrate the utility of the carbon emission detector, three sugars (glucose, fructose, sucrose) are determined in apple, crangrape, and orange juice.

Nonmetals in the argon–inductively coupled plasma–optical emission spectrometry: I. Phosphorus, sulfur and carbon

“…Table 1 lists the device parameters and experimental factors. The selection of the wavelengths was inspired by the published literature ( [3,4,5,6,7]) and the database of NIST (National Institute of Standards and Technology, USA). A set of up to ten lines was preselected and those that showed the highest sensitivity were selected for this work.…”

Simultaneous separation and determination of erythritol, xylitol, sorbitol, mannitol, maltitol, fructose, glucose, sucrose and maltose in food products by high performance liquid chromatography coupled to charged aerosol detector

“…Various analytical methods have been reported for the determination of sugars and sugar alcohols, including gas chromatography [15][16], the high performance liquid chromatography (HPLC) [1, [16][17][18][19] and capillary electrophoresis (CE) [20]. Gas [19,21].…”

Mesoporous Platinum Electrodes for Amperometric Determination of Sugars with Anion Exchange Chromatography

“…Although high-performance liquid chromatography (HPLC) with various modes is widely employed, [1][2][3][4][5][6] the detection of sugars is still a troublesome task. Since sugars lack native chromophores and fluorophores, they should be pre-reacted with proper derivatizing agents in order to employ a common UV or fluorescence detector.…”

“…Therefore, refractive index detection as a universal detector has been occasionally used in sugar analysis, but it is limited by poor sensitivity. 9 Amperometric detection in HPLC is well established for many easily oxidized or reduced organic analytes. However, sugars are not easily oxidized at commonly used glassy carbon and carbon paste electrodes.…”