An injectable heparin-conjugated hyaluronan scaffold for local delivery of transforming growth factor β1 promotes successful chondrogenesis

Abstract

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Bioprinting of Cartilaginous Auricular Constructs Utilizing an Enzymatically Crosslinkable Bioink

“…[ 12 ] This platform allows for the design of biomaterial systems mimicking the biomolecular characteristics of native tissue in either natural or synthetic hydrogels. [ 12b,13 ] Modulation of natural features, such as bioactive peptides, [ 13b ] signaling molecules, [ 14 ] proteolytic sequences and adhesive ligands, [ 15 ] as well as modular architectures linking different hydrogel systems such as polyethylene glycol (PEG), chondroitin sulfate, [ 14b ] gelatin, [ 16 ] hyaluronan, [ 17 ] and heparin [ 18 ] are thus possible. The innate biocompatibility of many enzymatic crosslinking processes [ 19 ] further overcomes concerns regarding cytotoxicity of chemical crosslinking processes such as in the case of photopolymerization where exposure to UV‐radiation and free radicals can be detrimental to cell viability.…”

Development of Standardized Fetal Progenitor Cell Therapy for Cartilage Regenerative Medicine: Industrial Transposition and Preliminary Safety in Xenogeneic Transplantation

“…Non-toxic and no angiogenesis perturbation in CAM 5 model 1 Non-immunogenic and non-tumorigenic in murine and rat models of cartilage defect or subcutaneous implantation Non-immunogenic, non-tumorigenic, and no slowing of cartilage defect healing in caprine model 1 [36,92] Functionality Spontaneous chondrogenic activity in 3D micropellets Potent and stable production of ECM 6 (i.e., GAGs 7 , aggrecan, types I and II collagen) Important functional responsiveness to mechanostimulation in dynamic scaffold culture conditions Highly responsive chondrogenic potential under biochemical stimulation (e.g., alginate, TGF-β1) Highly responsive chondrogenic potential in specific formulations (e.g., therapeutic cell microencapsulation) [12,34,36,70,92,94] Overall, the most interesting aspect of the Swiss FPC transplantation program is the initial requirement for only one single organ donation following a controlled and medically indicated pregnancy termination, for the parallel establishment of progenitor cell types from different tissues, among which is epiphyseal cartilage. From the same initial organ donation (i.e., FE002, 2009), dermal progenitor cell sources had been established, and the overall framework biobanking model was established and validated using this source in industrialized and clinical settings [30,41].…”

Holistic Approach of Swiss Fetal Progenitor Cell Banking: Optimizing Safe and Sustainable Substrates for Regenerative Medicine and Biotechnology

“…of the cells, which enable load bearing after successful implantation and integration (Hollister, 2005;Kemppainen and Hollister, 2010). External or internal biochemical modulation, specific processing (e.g., microgel encapsulation), and scaffold mechanical stimulation differentially constitute potent cues for chondrogenesis and structural or functional improvement in bioengineered constructs (Huang et al, 2005(Huang et al, , 2010Campbell et al, 2006;Terraciano et al, 2007;Levinson et al, 2019; FIGURE 11 | Clinical case-reports highlighting the efficacy of primary FPCs and derivatives thereof for the management of human acute and chronic cutaneous affections. (A1-A6) Refractory painful post-thrombotic ulcer lesions were treated weekly with Progenitor Biological Bandages and evolutive photographic representations were acquired at the time of treatment initiation, 11 weeks later, and 15 months later for follow-up.…”