An innovative biocatalyst for production of ethanol from xylose in a continuous bioreactor

Silva, C.R.; Zangirolami, Teresa Cristina; Rodrigues, Josemar; Matugi, Karina; Giordano, Roberto; Giordano, Raquel de Lima Camargo

doi:10.1016/j.enzmictec.2011.09.005

Cited by 36 publications

(22 citation statements)

References 30 publications

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“…While cellulolytic enzymes for efficient transformation of cellulose have been thoroughly described over the last decade in several different examples, the use of hemicellulose and lignin as substrate is a more incipient technology [44]. Several papers discuss the feasibility of integrating 2G ethanol into conventional ethanol producing units [45][46][47][48][49], but as of yet, there are no commercial plants available.…”

Section: Second Generation Fuel: Bioethanolmentioning

confidence: 99%

Green chemistry, sustainable agriculture and processing systems: a Brazilian overview

Perlatti

Forim

Zuin

2014

Chem. Biol. Technol. Agric.

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There is a pressing need for renewable and optimal use of resources towards sustainable primary production and processing systems worldwide. Current technologies for food and feedstock production are held accountable for several environmental problems, such as for instance soil and water contamination due to the use of hazardous substances, generation of toxic products and even excess of biomass that is considered waste. To minimize or solve these questions in order to produce an adequate quantity of reliable and healthy food, fibers and other products and energy, new paradigms focusing on sustainable agriculture, bio-based industries or biorefineries have emerged over the last decades. Biorefineries integrate sustainable and environmentally friendly concepts of Green Chemistry with intelligent and integrated farming processes, optimizing the agricultural production. Thermochemical and biochemical processes are excellent alternatives for the production of new classes of renewable biofuels and feedstock, showing relatively small impact on greenhouse gas emissions and important pathways to obtain platform chemicals. This review discusses the current and incipient technological developments for using biomass to generate bio-based chemicals over the last decade, focusing on Green Chemistry concepts towards sustainable agriculture and processing models in Brazil.

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Section: Second Generation Fuel: Bioethanolmentioning

confidence: 99%

Green chemistry, sustainable agriculture and processing systems: a Brazilian overview

Perlatti

Forim

Zuin

2014

Chem. Biol. Technol. Agric.

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“…A quitosana é um material abundante e de baixo custo utilizado em estudos para imobilização de diversas enzimas (Silva et al, 2012;Mendes et al, 2011;Kumar, 2000). Além de suas características vantajosas e de seu baixo custo, sua utilização pode ainda significar uma alternativa interessante para o aproveitamento dos resíduos das industrias de frutos do mar (Rodrigues et al, 2008).…”

Section: Resultsunclassified

“…A quitosana é um biopolímero obtido a partir da desacetilação da quitina de fungos ou crustáceos que apresenta grande potencial como suporte para a imobilização de enzimas por ser um polissacarídeo abundante e barato (Silva et al, 2012). A quitosana é biocompatível, biodegradável e possui uma elevada densidade de grupos amina primária que podem ser modificados quimicamente formando grupos glioxil extremamente reativos (Kumar, 2000).…”

Section: Introductionunclassified

IMOBILIZAÇÃO E ESTABILIZAÇÃO DE XILANASE RECOMBINANTE DE Bacillus subtilis EM QUITOSANA-GLIOXIL

Tardioli¹,

Milessi²,

Kopp³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -O aproveitamento da fração hemicelulósica da biomassa é essencial para a viabilidade da utilização de materiais lignocelulósicos em bioprocessos. As xilanases hidrolisam ligações glicosídicas entre unidades de xilose da hemicelulose, podendo ser aplicadas na etapa de pré-tratamento para liberação de açúcares fermentescíveis. A imobilização de xilanase é uma alternativa interessante, pois pode reduzir o custo da etapa de pré-tratamento. A quitosana-glioxil é um suporte de imobilização promissor devido ao seu baixo custo e por permitir a imobilização de enzimas por interações covalentes multipontuais. Neste contexto, o presente trabalho utilizou quitosana-glioxil para imobilizar endoxilanase recombinante de B. subtilis expressa em E. coli DH5α. A imobilização foi realizada à pH 10.0 e 25 ºC. O rendimento de imobilização foi de 76% com uma atividade recuperada de 53.6%. A enzima imobilizada apresentou meia vida de 5.9 h à 56 ºC e pH 5.5, sendo 75x mais estável que a enzima solúvel.

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“…Isomerization of xylose to xylulose is an essential step in this process. This reaction is in turn catalyzed by GI (Silva et al, 2012). Recently, attempts to improve the enzyme activity of the enzyme have been done, and the mutants enzymes showed remarkable increase in activity over a temperature range of 40 to 90°C at pH 7.0 (Wei et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Glucose (xylose) isomerase production from thermotolerant and thermophilic bacteria

Abdel-rassol¹,

Badr²,

Omran³

2012

Afr. J. Biotechnol.

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Glucose (xylose) isomerase (GI) is one of the most important industrial enzymes. It is used widely to catalyze the reversible conversion of D-glucose to D-fructose in vivo.The latter is used on a wide scale in the production of the high fructose corn syrup (HFCS) from corn starch. The great need of a thermostable GI, which is still active at higher temperatures (up to 90°C), opened the door to screen more microorganisms for the production of a more efficient industrial enzyme. Whole cells of 50 thermotole-rant/thermophilic bacterial isolates were used to evaluate their potential to produce GI when they were grown in broth medium (pH 7.0) containing D-xylose as a sole carbon source. Klebsiella and Pseudomonas showed the highest enzyme activity of 0.7; Bacillus came second with an activity of 0.3; while Acetobacter and Staphylococcus showed moderate activity of 0.3 for both, and Clostridium, Corynebacterium and Enterobacter showed the lowest enzyme activity of 0.2 each. The results reveal the need for optimizing the conditions for enhancing the production of the enzyme from Klebsiella and Pseudomonas isolates.

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An innovative biocatalyst for production of ethanol from xylose in a continuous bioreactor

Cited by 36 publications

References 30 publications

Green chemistry, sustainable agriculture and processing systems: a Brazilian overview

Green chemistry, sustainable agriculture and processing systems: a Brazilian overview

IMOBILIZAÇÃO E ESTABILIZAÇÃO DE XILANASE RECOMBINANTE DE Bacillus subtilis EM QUITOSANA-GLIOXIL

Glucose (xylose) isomerase production from thermotolerant and thermophilic bacteria

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