2022
DOI: 10.1002/cam4.5557
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An innovative single‐base extension method for synchronous detection of point mutations and MSI status in colorectal cancer

Abstract: Background An accurate genotyping analysis is one of the critical prerequisites for patients with colorectal cancer receiving matched therapies. Conventional genotyping analysis is currently used to detect either gene mutations or MSI status, delaying the detection of critical tumor biomarkers and thus the optimal time for treatment. An assay that analyzes both biomarkers in a streamlined process is eagerly needed. Methods We developed an assay combining Multiplex PCR Amplification, Single‐base Extension and c… Show more

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“…In this study, the mf‐ARMS PCR method of multiplexing the 10 loci was developed for the first time, which resulted in successful identification of wild and mutant alleles in capillary electrophoresis (Figure 2). Multiplex ARMS PCR has been used in human to identify mutations of Wilson disease (Dastsooz et al., 2013), severe acute respiratory syndrome coronavirus 2 (Islam et al., 2021), human colorectal cancer (Liang et al., 2023) and phenylketonuria (Zschocke & Graham, 1995).…”
Section: Locus Chromosome Gene Mutation and Type Of Varianta Mf‐arms‐...mentioning
confidence: 99%
“…In this study, the mf‐ARMS PCR method of multiplexing the 10 loci was developed for the first time, which resulted in successful identification of wild and mutant alleles in capillary electrophoresis (Figure 2). Multiplex ARMS PCR has been used in human to identify mutations of Wilson disease (Dastsooz et al., 2013), severe acute respiratory syndrome coronavirus 2 (Islam et al., 2021), human colorectal cancer (Liang et al., 2023) and phenylketonuria (Zschocke & Graham, 1995).…”
Section: Locus Chromosome Gene Mutation and Type Of Varianta Mf‐arms‐...mentioning
confidence: 99%