An insertion/deletion polymorphism in the interleukin-1A 3'untranslated region confers risk for gastric cancer

Zhang, Jinkun; Shi, Huihai; Xue, Mei; Yu, Qiang; Liu, Yang; Zheng, Shudan; Zhou, Chunxiao

doi:10.3233/cbm-160574

Cited by 9 publications

(16 citation statements)

References 38 publications

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“…We selected the rs3783553 according to the following criteria: (1) rs3783553 located in the 3’-UTR of IL-1A , with a function of disrupting the binding site of miR-122 and miR-378 [ 16 , 29 ] ; (2) IL-1α is a critical mediator of inflammatory responses in IS; and (3) miR-122 may be used as a biomarker in IS, involving in the process of postischemic neuronal damage. [ 16 – 18 ] From each subject, 2 to 3 mL venous blood sample was collected.…”

Section: Methodsmentioning

confidence: 99%

Functional polymorphism rs3783553 in the 3’-untranslated region of IL-1A increased the risk of ischemic stroke

Wang

Liu

et al. 2017

Medicine

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Accumulating evidence indicates interleukin-1 (IL-1) is a critical mediator of inflammatory responses in ischemic stroke (IS). The aim of this study was to investigate whether rs3783553 in the 3’-untranslated region of IL-1A was associated with the risk of IS. In this hospital-based case-control study, we genotyped the rs3783553 using polymerase chain reaction in 316 patients with IS and 332 age, sex, and ethnicity-matched controls. Plasma level of IL-1α was measured by enzyme-linked immunosorbent assay. The relative luciferase activities were measured by the Dual Luciferase assay system. The presence of ins/ins genotype was associated with higher odds ratios (ORs) of IS compared with del/del genotype (ins/ins vs del/del: adjusted OR 1.77, 95% confidence interval [CI] 1.06–2.98; recessive model: adjusted OR 1.69, 95% CI 1.06–2.70). The higher risk of IS was also observed in allele comparison (adjusted OR 1.29, 95% CI 1.00–1.65). Multivariate logistic regression analysis showed that age, hypertension, total cholesterol, triglyceride, low-density lipoprotein, and rs3783553ins/ins genotypes were independent risk factors for IS. Plasma level of IL-1α was higher among IS patients compared with controls (P = .03). Notably, IS patients with the TTCA/TTCA genotype had a higher level of IL-1α compared with those with the del/del genotype (P = .01). Luciferase reporter assay showed that the vector containing the TTCA del allele exhibited a reduced transcriptional activity in the presence of miR-122 and miR-378. These findings indicate that IL-1A rs3783553 ins/ins genotype may increase the susceptibility to IS, possibly by interrupting the binding site of miR-122 and miR-378.

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Section: Methodsmentioning

confidence: 99%

Functional polymorphism rs3783553 in the 3’-untranslated region of IL-1A increased the risk of ischemic stroke

Wang

Liu

et al. 2017

Medicine

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“…In term of rs3783553, it is a contention of a hundred schools of thought. It includes researches on multiple systems of cancer diseases, such as hepatocellular carcinoma, nasopharyngeal cancer, glioma, prostate cancer, gastric cancer, thyroid carcinoma, oral squamous cell carcinoma, cervical cancer, ovarian cancer, colorectal cancer, colorectal cancer, and endometrial cancer [37,[45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61] . Analyzed by ethnicity, most of studies focus on Asians, yet researches direct to Caucasians are also quite a lot.…”

Section: Literature Searches and Characteristicsmentioning

confidence: 99%

Effect of polymorphism on IL1A to cancer susceptibility: Evidence based on 34,016 subjects

Xia

Chen

Meng

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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IL-1a is closely related to the development and metastasis of cancer, and its polymorphisms have been reported affecting the susceptibility of malignancy tumors, yet the conclusions are controversial. Present an overall meta-analysis was performed to reach more general findings. Relevant literature was searched from Google Scholar, Web of Science, PubMed, EMBASE, CNKI database and Wanfang database, and the association among polymorphism and cancer risk was appraised by counting ORs and 95% CIs of overall and stratification analysis. The date from 15,586 cases and 18,430 controls in 40 publications were enrolled. There is significantly upregulated risk leads by rs3783553 in all genetic models, while the same tendency was found in all cancer types. The results also suggest a high risk of cancer susceptibility in patients carried rs1800587 polymorphism, of which draw out form allelic and homozygote models in overall studies, especially for cervical cancer. However, there are no significant results in analysis of rs17561. In a word, IL1A SNPs play an essential role in upregulating cancer susceptibility, and current analysis provides detail date for further study in the future.

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“…Genome‐wide analysis has revealed that small insertion/deletions (INDELs) are the second most common type of genetic variants after single nucleotide polymorphisms (SNPs) in the human genome and they are likely to have a significant impact on human biology and diseases . Some studies have suggested that some INDELs are related to many cancers including gastric and hepatocellular carcinoma . In this regard, rs199971565 is an INDEL located in miR‐302c, which may affect its function and biogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…19 Some studies have suggested that some INDELs are related to many cancers including gastric and hepatocellular carcinoma. 20,21 In this regard, rs199971565 is an INDEL located in miR-302c, which may affect its function and biogenesis. To the best of our knowledge, no genetic association study has been conducted on this INDEL with any disease by now.…”

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confidence: 99%

Association and in silico investigations of miR‐302c insertion/deletion variant as a novel biomarker with susceptibility to gastric cancer

Raad

Bayat

Sharafshah

et al. 2019

J of Cellular Biochemistry

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Gastric cancer (GC) is the fifth most prevalent malignant tumor and the third most frequent cause of cancer mortality worldwide. rs199971565 is an insertion/deletion (INDEL) located in microRNA‐302c (miR‐302c) seed site, which may affect its function and biogenesis. There is no genetic association study investigating this INDEL with any disease till now. Thus, the current study was conducted to investigate the association of rs199971565 with susceptibility to GC in an Iranian population. In addition, in silico studies were performed to reveal the possible functional significance of this INDEL. A total of 378 subjects were genotyped through amplification refractory mutation system PCR (ARMS‐PCR) after DNA extraction from peripheral blood by the salting out procedure. Also, in silico analyses were performed through databases and web tools including MiRNASNP V2.0, miRWalk V2.0, miRTarBase, DAVID V6.8, RNAfold, PHDcleave, miRmap, and STarMir. Results revealed that there was an association between rs199971565 and the incidence risk of GC under a recessive (P = .04, odds ratio [OR] = 18.73; 95% confidence interval [CI] = 1.07‐326.95) model of inheritance. Also, compared to the Ins allele, the Del allele significantly increased the risk of GC (P = .01, OR = 2.02; 95% CI = 1.11‐3.66). Further analyses showed no significant association in age and sex between two study groups (P = .216 and P = .798, respectively). In conclusion, for the first time, this study indicated the association and in silico investigations of rs199971565 and suggested it as a novel INDEL biomarker located in the seed site of miR‐302c, which may have crucial roles in the susceptibility to GC and its incidence risk.

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An insertion/deletion polymorphism in the interleukin-1A 3'untranslated region confers risk for gastric cancer

Abstract: These findings suggest that functional polymorphism rs3783553 in IL-1A could contribute to GC susceptibility, possibly or at least partially through affecting the transcriptional activity of IL-1A.

Cited by 9 publications

References 38 publications

Functional polymorphism rs3783553 in the 3’-untranslated region of IL-1A increased the risk of ischemic stroke

Functional polymorphism rs3783553 in the 3’-untranslated region of IL-1A increased the risk of ischemic stroke

Effect of polymorphism on IL1A to cancer susceptibility: Evidence based on 34,016 subjects

Association and in silico investigations of miR‐302c insertion/deletion variant as a novel biomarker with susceptibility to gastric cancer

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