An insight into chiral monolithic stationary phases for enantioselective high‐performance liquid chromatography applications

Abstract

In this review, three main classes of chiral monolithic stationary phases, namely silica-, organic polymer-, and hybrid-based monolithic stationary phases, are covered. Their preparations, applications, and advantages compared with the conventionalpacked and open-tubular capillary columns are discussed. A detailed description of the different types and techniques used for the introduction of chiral selectors into the monolithic matrices such as immobilization, functionalization, coating, encapsulation, and bonding. Special emphasis is given to the recent developments of chiral selectors in HPLC monolithic stationary phases during the past 18 years.

RP-HPLC stability-indicating method for simultaneous determination of sodium valproate, methylparaben and propylparaben in oral solution

“…Because of its unique features, as it is economically and environmentally benign than other methods, RP-HPLC method was used here [21][22][23][24][25][26]. The major advantage of the proposed method is that the assay of sodium valproate and preservatives can be achieved in presence of their stress degradation products on a single chromatographic system with UV detection without the need for prior derivatization.…”

Hybridization of helical poly(phenylacetylene)s bearing l-proline tripeptide pendants into porous silica microspheres as a solvent-tolerable chiral stationary phase for liquid chromatography

“…The resulting HCSP demonstrated excellent chiral recognition ability because the chiral recognition units were widely distributed in the carrier. [28][29][30] In this study, a novel phenylacetylene derivative containing a proline tripeptide pendant (PA-P 3 -ee) was synthesized and copolymerized with a phenylacetylene derivative containing 3-(triethoxysilyl)propyl groups to obtain a novel copoly( phenylacetylene) polymer (CPA). CPA was introduced during the growth of SiO 2 PMSs such that the CPA units were efficiently hybridized into the SiO 2 PMSs during the hydrolytic polycondensation of ethoxysilyl groups to obtain chiral hybrid microspheres with high loading amounts of the chiral recognition units.…”

Chiral separation using cyclodextrins as mobile phase additives in open‐tubular liquid chromatography with a pseudophase coating

“…Open‐tubular LC (OT‐LC) uses a chromatographic coating on the walls of narrow inner diameter columns [ 17 , 18 , 19 , 20 , 21 , 22 ]. OT‐LC separations may offer advantages such as low sample and consumable requirements, zero backflow, and no column clogging.…”