An insight into normal and pathological pregnancies using large-scale microarrays: lessons from microarrays

Chaouat, G.; Rodde, Nathalie; Petitbarat, Marie; Bulla, Roberta; Rahmati, Mona; Dubanchet, Sylvie; Zourbas, Sandrine; Bataillon, Isabelle; Coqué, Nathalie; Hennuy, Benoît; Martal, J.; Munaut, Carine; Aubert, Julie; Sérazin, Valérie; Steffen, T. Simon; Jensenius, Jens Christian; Foidart, Jean Michel; Sandra, Olivier; Tedesco, Francesco; Lédée, Nathalie

doi:10.1016/j.jri.2010.12.006

Cited by 7 publications

(7 citation statements)

References 42 publications

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“…MBL deposition was followed by C3 observed at day 6.5 of pregnancy in agreement with a similar observation of Girardi et al (9), who also found that C3 appeared at the same time as the proinflammatory cells monocytes and polymorphonuclear leukocytes. Our own observation that cytokines involved in pregnancy loss including IFN-g, TNF-a, and IL-10 are not expressed earlier than 6.5 d of pregnancy is consistent with this kinetic (30). Taken together, these data suggest that C may be the initiator of the complex series of events mediated by multiple pathogenic factors leading to adverse pregnancy outcome in this murine mating combination.…”

Section: Discussionsupporting

confidence: 80%

Critical Role and Therapeutic Control of the Lectin Pathway of Complement Activation in an Abortion-Prone Mouse Mating

Petitbarat

Durigutto

Macor

et al. 2015

The Journal of Immunology

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The abortion-prone mating combination CBA/J × DBA/2 has been recognized as a model of preeclampsia, and complement activation has been implicated in the high rate of pregnancy loss observed in CBA/J mice. We have analyzed the implantation sites collected from DBA/2-mated CBA/J mice for the deposition of the complement recognition molecules using CBA/J mated with BALB/c mice as a control group. MBL-A was observed in the implantation sites of CBA/J × DBA/2 combination in the absence of MBL-C and was undetectable in BALB/c-mated CBA/J mice. Conversely, C1q was present in both mating combinations. Searching for other complement components localized at the implantation sites of CBA/J × DBA/2, we found C4 and C3, but we failed to reveal C1r. These data suggest that complement is activated through the lectin pathway and proceeds to completion of the activation sequence as revealed by C9 deposition. MBL-A was detected as early as 3.5 d of pregnancy, and MBL-A deficiency prevented pregnancy loss in the abortion-prone mating combination. The contribution of the terminal complex to miscarriage was supported by the finding that pregnancy failure was largely inhibited by the administration of neutralizing Ab to C5. Treatment of DBA/2-mated CBA/J mice with Polyman2 that binds to MBL-A with high affinity proved to be highly effective in controlling the activation of the lectin pathway and in preventing fetal loss.

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Section: Discussionsupporting

confidence: 80%

Critical Role and Therapeutic Control of the Lectin Pathway of Complement Activation in an Abortion-Prone Mouse Mating

Petitbarat

Durigutto

Macor

et al. 2015

The Journal of Immunology

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“…Numerous studies have identified altered gene expression in the placentas of mothers affected by preeclampsia [5], [6], [7], [8], [9]. Preeclampsia-specific altered gene expression has also been reported in chorionic villus samples obtained in early gestation from pre-symptomatic mothers who went on to develop preeclampsia [10], [11].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Preeclampsia-Associated DNA Methylation in the Placenta

et al. 2014

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BackgroundA small number of recent reports have suggested that altered placental DNA methylation may be associated with early onset preeclampsia. It is important that further studies be undertaken to confirm and develop these findings. We therefore undertook a systematic analysis of DNA methylation patterns in placental tissue from 24 women with preeclampsia and 24 with uncomplicated pregnancy outcome.MethodsWe analyzed the DNA methylation status of approximately 27,000 CpG sites in placental tissues in a massively parallel fashion using an oligonucleotide microarray. Follow up analysis of DNA methylation at specific CpG loci was performed using the Epityper MassArray approach and high-throughput bisulfite sequencing.ResultsPreeclampsia-specific DNA methylation changes were identified in placental tissue samples irrespective of gestational age of delivery. In addition, we identified a group of CpG sites within specific gene sequences that were only altered in early onset-preeclampsia (EOPET) although these DNA methylation changes did not correlate with altered mRNA transcription. We found evidence that fetal gender influences DNA methylation at autosomal loci but could find no clear association between DNA methylation and gestational age.ConclusionPreeclampsia is associated with altered placental DNA methylation. Fetal gender should be carefully considered during the design of future studies in which placental DNA is analyzed at the level of DNA methylation. Further large-scale analyses of preeclampsia-associated DNA methylation are necessary.

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“…"genetic background" versus 12.2%), as usually observed in conventional "clean" conditions [90,91]. Interestingly, MBL deregulations were also observed in human.…”

Section: The Complementmentioning

confidence: 76%

“…To gain further insights in uterine modifications in implantation we performed microarrays in the CBA × DBA/2 vs. CBA × BALB/ matings in gnotobiotic conditions [91].…”

Section: Microarrays In Micementioning

confidence: 99%

Evolution of (our) Concepts on Key Determinants of a Successful Pregnancy

Chaouat

Petit

Petitbarat

et al. 2011

Advances in Neuroimmune Biology

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In this paper, I outline briefly the concepts that led to the evolution of Reproductive Immunology. The history starts in 1971, with the "Medawar paradigm", and as a consequence in the 70 s-90 s, the placental barrier and immunosuppression concepts were proposed. The first conceptual breakthrough started with the "vaccination against abortion in mice", leading to immunotrophysm, and the importance of cytokines emerged, which lead to the disapproval of the Th1:Th2 paradigm. It was necessary to revise this approach, and, notably, it was recognized that NK cells were not only "bad guys", but in fact were required for successful pregnancy. NK cells are involved in the transformation of the local vessels. We are interest in the control of NK activation by various interleukins (notably the Il-12, IL-18, NK tripod) as well as tumour necrosis factor-related weak inducer of apoptosis (TWEAK) in mice and human.One must now recognize "suppression" as Tregs emerged, and recognize signals of the embryo. We also discuss here FF G-CSF and sHLA-G. Microarrays, as well as studies on innate immunity, which are amongst our latest interests, while combining studies in mice and human, with spin off consequences for the clinician.

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An insight into normal and pathological pregnancies using large-scale microarrays: lessons from microarrays

Cited by 7 publications

References 42 publications

Critical Role and Therapeutic Control of the Lectin Pathway of Complement Activation in an Abortion-Prone Mouse Mating

Critical Role and Therapeutic Control of the Lectin Pathway of Complement Activation in an Abortion-Prone Mouse Mating

Comprehensive Analysis of Preeclampsia-Associated DNA Methylation in the Placenta

Evolution of (our) Concepts on Key Determinants of a Successful Pregnancy

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