An insight into the genome of extensively drug-resistant and uropathogenic Citrobacter werkmanii

Parvez, Shadab; Khan, Asad U.; Kaur, Gagandeep; Barakat, Mohamed; Ortet, Philippe; Mayilraj, Shanmugam

doi:10.1016/j.jgar.2020.06.014

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…These Citrobacter species can also be located in food, soil and as well as in water (Rogers et al, 2015). The C. werkmanii AK-8 strain, separated from a stool sample in a hospital setting, exhibits the presence of antibiotic resistance genes, as confirmed by its complete genomic analysis (Parvez et al, 2020). Through the investigation of the AK-8 genome, some researchers discovered the presence of the two-component system of BaeSR, which controls the synthesis of efflux proteins (multidrug).…”

Section: Introductionmentioning

confidence: 91%

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From Sequence to Significance: A Thorough Investigation of the Distinctive Genome Traits Uncovered inC. werkmaniistrain NIB003

Hossain,

Tanvir,

Chowdhury

et al. 2023

Preprint

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Citrobacter werkmanii(C. werkmanii) is an opportunistic bacterium that has received very little investigation in Bangladesh, even though it is thought to be capable of causing diseases including diarrhea and urinary tract infections. Therefore, the present study focuses on the exploration of genomic features, including antibiotic resistance genes, virulent genes, and their distinctive nature in the pathogenesis of diseases. A complete genome was sequenced for the first time in Bangladesh after the morphological, biochemical and molecular identification of this bacteria. A total of 47,65,861 nucleotide base pairs makes up the genome, which contains 4669 genes, and many of the protein-coding genes can be classified into various ontological frameworks, including COG and KEGG pathways. Notably,C. werkmaniihas the capability to have interactions withEscherichia coli, Salmonella,andKlebsiellawhile they possess the same domains and motifs, indicating a causal role in the development of diarrhea. Furthermore, the Bangladeshi isolate ofC. werkmaiihas observed genetic heterogeneity when compared to other strains ofC. werkmanii: C. werkmanii FDAARGOS_616, C. werkmanii LCU-V21, andC. werkmanii BF-6. This identified genetic variation of sequencedC. werkmaniicould drive the functional aberration in body homeostasis of bacteria. Later, molecular dynamics studies have proven the impact of their genetic variation on the protein structure, as indicated by the SASA, RMSD, Rg, and RMSF values. Finally, the findings ofC. werkmaniifrom the sequenced genome might provide us with a better understanding ofC. werkmaniiand their possible role as opportunistic bacteria in diseases including diarrhea and urinary tract infections.

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Section: Introductionmentioning

confidence: 91%

“…Additionally, it was discovered that Bar-UvrY has a role in controlling flagellum synthesis, the metabolism of carbon, and the formation of biofilm. The genome of C. werkmanii AK-8 harbors a set of 20-21 chemoreceptors, which play a crucial role in facilitating colonization and pathogenicity (Parvez et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

From Sequence to Significance: A Thorough Investigation of the Distinctive Genome Traits Uncovered inC. werkmaniistrain NIB003

Hossain,

Tanvir,

Chowdhury

et al. 2023

Preprint

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“…Given that Citrobacter can persist in a host for long periods [ 13 ], Citrobacter infections can result in multidrug resistance outbreaks among neonates and immunocompromised patients who have prolonged hospital stays [ 8 , 10 , 14 , 15 , 16 , 17 ]. Recent evidence has demonstrated that other Citrobacter species, such as Citrobacter werkmanii , are also considered emerging opportunistic pathogens in developing countries [ 9 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although considered to be commensal in humans and animals, C. werkmanii has previously been linked as a causative agent of wound infection, urinary tract infections, and bacteremia in humans [ 18 , 19 ]. More recently, the use of whole-genome sequencing has revealed that the C. werkmanii strain AK-8, isolated from a patient suffering chronic kidney disease, harbors genes with a functional role in virulence and antimicrobial resistance [ 20 ], highlighting the need for subsequent studies to further characterize the virulence potential of this commensal as an opportunistic human pathogen. To expand on the characterization of C. werkmanii as an emerging opportunistic pathogen, the present study conducted comparative genomic analyses of C. werkmanii strains recovered from a major agricultural region producing horticultural products in Mexico, as well as from various geographical locations and sources, to identify relevant virulence and antimicrobial resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomic Analyses of Virulence and Antimicrobial Resistance in Citrobacter werkmanii, an Emerging Opportunistic Pathogen

Aguirre-Sánchez,

Quiñones,

Ortiz-Muñoz

et al. 2023

Microorganisms

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Citrobacter werkmanii is an emerging and opportunistic human pathogen found in developing countries and is a causative agent of wound, urinary tract, and blood infections. The present study conducted comparative genomic analyses of a C. werkmanii strain collection from diverse geographical locations and sources to identify the relevant virulence and antimicrobial resistance genes. Pangenome analyses divided the examined C. werkmanii strains into five distinct clades; the subsequent classification identified genes with functional roles in carbohydrate and general metabolism for the core genome and genes with a role in secretion, adherence, and the mobilome for the shell and cloud genomes. A maximum-likelihood phylogenetic tree with a heatmap, showing the virulence and antimicrobial genes’ presence or absence, demonstrated the presence of genes with functional roles in secretion systems, adherence, enterobactin, and siderophore among the strains belonging to the different clades. C. werkmanii strains in clade V, predominantly from clinical sources, harbored genes implicated in type II and type Vb secretion systems as well as multidrug resistance to aminoglycoside, beta-lactamase, fluoroquinolone, phenicol, trimethoprim, macrolides, sulfonamide, and tetracycline. In summary, these comparative genomic analyses have demonstrated highly pathogenic and multidrug-resistant genetic profiles in C. werkmanii strains, indicating a virulence potential for this commensal and opportunistic human pathogen.

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Whole-genome sequencing of bacteria accountable for lactational mastitis in humans combined with an examination of their antibiotic resistance profiles

Dalwadi,

Nathani,

Chauhan

et al. 2024

Braz J Microbiol

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An insight into the genome of extensively drug-resistant and uropathogenic Citrobacter werkmanii

Cited by 6 publications

References 38 publications

From Sequence to Significance: A Thorough Investigation of the Distinctive Genome Traits Uncovered inC. werkmaniistrain NIB003

From Sequence to Significance: A Thorough Investigation of the Distinctive Genome Traits Uncovered inC. werkmaniistrain NIB003

Comparative Genomic Analyses of Virulence and Antimicrobial Resistance in Citrobacter werkmanii, an Emerging Opportunistic Pathogen

Whole-genome sequencing of bacteria accountable for lactational mastitis in humans combined with an examination of their antibiotic resistance profiles

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