This study aims to investigate the relationship between Total Quality Management (TQM) practices and quality culture in Kingdom of Saudi Arabia. The samples comprised 388 samples and data was collected through a questionnaire from the managers of leading construction companies in the Kingdom of Saudi Arabia. The respondents were requested to answer self-administered questionnaires, and send their feedback to the researcher, where 5 points rating scale was used to elaborate on varying responses. Additionally, this study employed multiple regression analysis in order to test the relationship between independent variables (TQM practices namely, education and training, customers focus, information and analysis, continuous improvement, process management and top management commitment) and dependent variable (quality culture). The results of this study revealed a positive relationship between education and training and quality culture. In the same path, the results revealed a positive association between customer focus and quality culture and a positive association between information and analysis and quality culture. Additionally, the results also revealed a positive relationship between continuous improvement and quality culture and a positive relationship between process management and quality culture. Finally, the study results showed a positive top management commitment-quality culture relationship. The study provided recommendations and directions for future research.