An Instrument-free Detection of Antioxidant Activity Using Paper-based Analytical Devices Coated with Nanoceria

Piyanan, Thirada; Athipornchai, Anan; Henry, Charles S.; Sameenoi, Yupaporn

doi:10.2116/analsci.34.97

Cited by 31 publications

(16 citation statements)

References 40 publications

(43 reference statements)

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“…Nanoparticles were employed for antioxidant activity analysis on the μPAD to determine the reducing power of an antioxidant toward metal nanoparticles. [35][36][37] The capacity of antioxidant to reduce Au 3+ to form gold nanoparticles was measured colorimetrically on paper. 37 The reducing power of the antioxidant was also determined by the nanoceria on the μPAD, where Ce 4+ on the nanoceria surface was reduced to Ce 3+ by the antioxidant, leading to a change in color from yellow to brown.…”

Section: Introductionmentioning

confidence: 99%

“…37 The reducing power of the antioxidant was also determined by the nanoceria on the μPAD, where Ce 4+ on the nanoceria surface was reduced to Ce 3+ by the antioxidant, leading to a change in color from yellow to brown. 35,36 The radical scavenging activity of the antioxidant has also been measured using the DPPH assay on μPADs. However, only two antioxidant standards, including gallic acid 38 and butylated hydroxytoluene 39 have been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Paper-based DPPH Assay for Antioxidant Activity Analysis

2018

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We report on a paper-based 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay for a simple, inexpensive, low reagent and sample consumption and high throughput analysis of antioxidant activity. The paper-based device was fabricated using a lamination method to create a 5-mm in diameter circular test zone that was embedded with a DPPH reagent. The analysis was carried out in one-step by dropping an antioxidant/sample onto the test zone. After reduction by the antioxidant, the DPPH radicals become stable DPPH molecules, resulting in a change in color from deep violet to pale yellow. The violet color intensity of DPPH was inversely proportional to the antioxidant activity of the samples, and was measured using imaging software. A high precision and a low limit of detection were found in the analysis of six standard antioxidants including gallic acid, trolox, ascorbic acid, caffeic acid, vanilliic acid and quercetin. The device was then validated against the traditional spectrophotometric DPPH assay by analyzing the antioxidant activity of 7 tea samples. The results showed no significant difference for gallic acid equivalent for all 7 samples obtained from the two methods at the 95% confidence level, indicating that the developed method was reliable for antioxidant activity analysis of real samples. Finally, the paper-based DPPH device was found to be stable over 10 days when stored in a refrigerator (2 - 4°C), making it an easy-to-use device for end-users.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Paper-based DPPH Assay for Antioxidant Activity Analysis

2018

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“…After Henry's group work, various novel distance‐based readout strategies have been developed for extending their wide applicability. For instance, different types of targets, such as metal ions (e.g., K + , Cu 2+ , and Hg 2+ ), bacteria (e.g., Salmonella typhimurium ), and antioxidants (e.g., ascorbic acid and gallic acid), have been directly detected on distance‐based paper devices in a visually quantitative manner with high sensitivity and selectivity, where the target analytes react with colorimetric agents to produce color change …”

Section: Distance‐based Quantitative Readout Strategymentioning

confidence: 99%

Equipment‐Free Quantitative Readout in Paper‐Based Point‐of‐Care Testing

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Bai

et al. 2019

Small Methods

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been widely applied in resource-limited settings due to their easy-to-operate and equipment-independent characteristics with minimum user intervention. [9] However, LFAs are often limited to qualitative results readout by providing "yes" or "no" signal, which is insufficient in some application scenarios, e.g., a clinical action will be made in clinics according to the accurate amount of targets. Recently, the emerging µPADs have gained considerable interests due to their excellent capability for outputting quantitative results. [10] Nowadays, quantitative paper-based assays are mainly based on electrochemical detection, [11] electrochemiluminescence detection, [12] and fluorescent detection. [13,14] Although sensitive and quantitative detection can be obtained, special equipment for quantification of detection signal is usually involved. For instance, electrochemical workstation is needed for performing electrochemical detection on paper, while a special light-emitting device is indispensable for fluorescent testing. For addressing these limitations, some handheld equipments, such as glucose meter, [15] multimeter, [16] and smartphone, [17,18] have been introduced as readers for outputting quantitative measurements with improved portability. However, the accessibility and cost of these handheld equipments may still be a big challenge for their application in resource-poor areas, where the quantitative detection in an equipment-free manner is preferred. Hence, the exploitation of equipment-free paper-based analytical devices shows broad practical application prospects in POCT field.Colorimetric detection holds great potential for developing equipment-free detection methods through direct readout by naked eye. [19] Conventionally, the color intensity analysis has been widely used to realize quantification of analytes, whereas a camera is needed to capture images and a computer-based software for image processing. [20] Hence, these quantitative methods based on color intensity analysis are unsatisfactory for exploiting equipment-free quantitative strategies. Fortunately, thanks to the novel signal transformation design in paper-based colorimetric detection, different types of equipment-free quantitative paper devices have already been proposed, where the detection signal has been transformed into the measurement of color change distance, [21] the counting of colored detection zones, [22] the measurement of testing time, [23] and the reading of displayed text. [24] These equipment-free strategies have been demonstrated to be capable of detecting a variety of analytes with acceptable performance, such as metal ions, nucleic acids, bacteria, and cells, whose detection shows great attraction in POCT, thus exhibiting great feasibility and versatility for POCT applications.Equipment-free paper-based devices with quantitative readout have gained considerable interests in recent years, due to the features of remarkable cheapness, prominent portability, and excellent user friendliness. These equipment-free strategies have b...

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“…A comparison of size and activity of various nanoparticles used in different diagnostic biomedical applications is provided in Table 1. 12,22…”

Section: Nanostructures and Nanoparticlesmentioning

confidence: 99%

Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics

2018

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In resource-limited settings, the availability of medical practitioners and early diagnostic facilities are inadequate relative to the population size and disease burden. To address cost and delayed time issues in diagnostics, strip-based immunoassays, e.g. dipstick, lateral flow assay (LFA) and microfluidic paper-based analytical devices (microPADs), have emerged as promising alternatives to conventional diagnostic approaches. These assays rely on chromogenic agents to detect disease biomarkers. However, limited specificity and sensitivity have motivated scientists to improve the efficiency of these assays by conjugating chromogenic agents with nanoparticles for enhanced qualitative and quantitative output. Various nanomaterials, which include metallic, magnetic and luminescent nanoparticles, are being used in the fabrication of biosensors to detect and quantify biomolecules and disease biomarkers. This review discusses some of the principles and applications of such nanoparticle-based point of care biosensors in biomedical diagnosis.

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An Instrument-free Detection of Antioxidant Activity Using Paper-based Analytical Devices Coated with Nanoceria

Cited by 31 publications

References 40 publications

Paper-based DPPH Assay for Antioxidant Activity Analysis

Paper-based DPPH Assay for Antioxidant Activity Analysis

Equipment‐Free Quantitative Readout in Paper‐Based Point‐of‐Care Testing

Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics

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