An integrase of endogenous retrovirus is involved in maternal mitochondrial DNA inheritance of the mouse

Hayashida, Kenji; Omagari, Katsuhisa; Masuda, Junichi; Kohno, Shigeru

doi:10.1016/j.bbrc.2007.11.127

Cited by 8 publications

(18 citation statements)

References 26 publications

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“…Eri15 has two monomers including 15 kDa, and its isoform or its post-translational modification of 18 kDa. Although a tetramer of 60 kDa and a trimer of 45 kDa have been expressed respectively in ovary and testis [4], dimers of 30 (15×2) kDa and 36 (18×2) kDa were further detected common to both in this research. Among these, only 36 kDa Eri15 was detected in the BS-F1 testis mitochondria that were processed with trypsin while Eri15 was not detected in Mus spretus testis mitochondria (Fig.…”

Section: Resultsmentioning

confidence: 50%

“…3B(a)), and thus the metaphase was thought to be an initial stage of arrest in type 2 hybrid testis. In immunohistochemical staining too both Spag1 and Eri15 (both form a complex in the ovary cytosol [4]) were detected in the cytoplasm of some metaphase spermatocytes in a similar pattern (Fig. 3B(c)(d)).…”

Section: Resultsmentioning

confidence: 57%

“…Western immunoblotting by SDS-PAGE of ovary and testis proteins with anti-Spag1-2 polyclonal antibody and anti-Eri15 polyclonal antibody was performed as previously described [3,4]. Anti-β-actin monoclonal antibody (Sigma) and anti-VDAC1/porin polyclonal antibody (abcam) were used as the 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 8 loading control of cytosol and mitochondria respectively.…”

Section: Western Immunoblotting Analysismentioning

confidence: 99%

“…After deparaffinizing and rehydrating the fixed and paraffin-embedded sections of BS-F1 testis, immunohistochemical staining with anti-Spag1-2 polyclonal antibody and anti-Eri15 polyclonal antibody was performed as previously described [4]. Normal rabbit serum was used as a negative control.…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

“…We previously reported that maternal mitochondrial DNA (mtDNA) inheritance of the mouse is through selective sperm mtDNA elimination based on the sperm-specific translocator of the mitochondrial outer membrane (Spag1-1), two egg factors of the system-specific endonuclease (Eri15) and the system-specific chaperone (Spag1-2 or 1-3) [3,4]. In the male germ cells the expression of Spag1-2 (or 1-3) is possibly suppressed by epistatic gene(s).…”

Section: Introductionmentioning

confidence: 99%

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Hybrid male sterility is caused by mitochondrial DNA deletion

Hayashida

Kohno

2008

Mol Biol Rep

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Section: Resultsmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 57%

Section: Western Immunoblotting Analysismentioning

confidence: 99%

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hybrid male sterility is caused by mitochondrial DNA deletion

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Mitochondrial inheritance in fungi

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Engineering a waste management enzyme to overcome cancer resistance to apoptosis: adding DNase1 to the anti-cancer toolbox

et al. 2011

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Cancer treatment is often complicated by resistance to conventional anti-cancer treatment and to more recently developed immunotherapy and gene therapy. These therapeutic modalities aim at activating death pathways within cancer cells. Attempts to activate the apoptotic death pathway, by overexpressing proapoptotic signals, are compromised by cancer defense mechanisms, which disrupt the apoptotic-signaling cascade downstream of the overexpressed component. Here, we describe a therapeutic option of triggering apoptosis without activating the apoptotic-signaling cascade or using the native apoptosis executioner nuclease. We have engineered Deoxyribonuclease-1 (DNase1), a waste-management enzyme, by deleting its signal peptide, adding a nuclear localization signal, and mutating its actin-binding site. Apoptosis studies and colony-forming assay for assessing cell viability were conducted in apoptosis-resistant Mel-Juso human melanoma cells. The modified DNase1 reduced cell viability by 77% relative to controls. It also induced typical microscopic features of cellular apoptosis, such as Terminal Transferase dUTP Nick-End Labelingpositive cells and DNA fragmentation. Quantification of apoptosis by Laser scanning cytometry demonstrated high-killing efficiency of 70-100%. The results suggest that this modified DNase1 can efficiently eliminate apoptosis-resistant cancer cells through apoptosis. Coupled to different tissue-specific gene expression elements, this recombinant DNase1 may serve as a platform for eliminating a variety of cancer types.

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An integrase of endogenous retrovirus is involved in maternal mitochondrial DNA inheritance of the mouse

Cited by 8 publications

References 26 publications

Hybrid male sterility is caused by mitochondrial DNA deletion

Hybrid male sterility is caused by mitochondrial DNA deletion

Mitochondrial inheritance in fungi

Engineering a waste management enzyme to overcome cancer resistance to apoptosis: adding DNase1 to the anti-cancer toolbox

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