An integrated all foil based micro device for point of care diagnostic applications

Bose, Indranil; Ohlander, Anna; Kutter, Christoph; Russom, Aman

doi:10.1016/j.snb.2017.12.133

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…Laser cutting was also used for patterning microfluidic structures in ceramic materials xurography, also called razor writing, has been used to create voids in polymer films . The latter gave rise to “lab-on-a-foil” devices, where multilayer structures often offer functionality, for example as waveguide, rather than providing 3D fluidics. Combining foils with different wetting characteristics structure by xurography, however, 3D fluidic geometries incorporating hydrophobic barriers were presented .…”

Section: Printing Microfluidic Structuresmentioning

confidence: 99%

3D Printing: An Alternative Microfabrication Approach with Unprecedented Opportunities in Design

et al. 2020

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In the past decade, 3D printing technologies have been adopted for the fabrication of microfluidic devices. Extrusionbased approaches including fused filament fabrication (FFF), jetting technologies including inkjet 3D printing, and vat photopolymerization techniques including stereolithography (SLA) and digital light projection (DLP) are the 3D printing methods most frequently adopted by the microfluidic community. Each printing technique has merits toward the fabrication of microfluidic devices. Inkjet printing offers a good selection of materials and multimaterial printing, and the large build space provides manufacturing throughput, while FFF offers a great selection of materials and multimaterial printing but at lower throughput compared to inkjet 3D printing. Technical and material developments adopted from adjacent research fields and developed by the microfluidic community underpin the printing of sub-100 μm enclosed microchannels by DLP, but challenges remain in multimaterial printing throughput. With the feasibility of 3D printed microfluidics established, we look ahead at trends in 3D printing to gain insights toward the future of this technology beyond the sole prism of being an alternative fabrication approach. A shift in emphasis from using 3D printing for prototyping, to mimic conventionally manufactured outputs, toward integrated approaches from a design perspective is critically developed.

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Section: Printing Microfluidic Structuresmentioning

confidence: 99%

3D Printing: An Alternative Microfabrication Approach with Unprecedented Opportunities in Design

et al. 2020

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“…Additionally, PC is available as flexible foil, which requires not only fewer resources but also handling can be simplified. Processes such as roll-to-roll fabrication can furthermore enhance the fabrication of microarray platforms [26].…”

Section: Introductionmentioning

confidence: 99%

Flow-Based CL-SMIA for the Quantification of Protein Biomarkers from Nasal Secretions in Comparison with Sandwich ELISA

et al. 2023

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Protein biomarkers in nasal secretions can be used as a measure to differentiate between allergies, airway diseases and infections for non-invasive diagnostics. The point-of-care quantification of biomarker levels using flow-based microarray facilitates precise and rapid diagnosis and displays the potential for targeted and effective treatment. For the first time, we developed a flow-based chemiluminescence sandwich microarray immunoassay (CL-SMIA) for the quantification of nasal interferon-beta (IFN-β) on the Microarray Chip Reader-Research (MCR-R). Polycarbonate foils are used as a cost-effective surface for immobilizing capture antibodies. By using a commercially available set of anti-human IFN-β antibodies, the CL-SMIA can be compared directly to an enzyme-linked immunosorbent assay (ELISA) performed in microtiter plates concerning the bioanalytical performance and economic issues. Pre-incubation of the sample with detection antibodies facilitates the lower consumption of detection antibodies, as this allows for a longer interaction time between the antibody and the biomarker. The direct injection of pre-incubated samples into the microarray chips eliminates the adsorption of proteins in the tubing as well as the contamination of the tubing and valves of the MCR-R with clinical samples. The small flow cell allows for a low sample volume of 50 μL. The limit of detection of 4.53 pg mL−1 was slightly increased compared to a sandwich ELISA performed on microtiter plates which were 1.60 pg mL−1. The possibility to perform the CL-SMIA in a multiplexed mode makes it a promising assay for the rapid and cost-effective non-invasive detection of biomarkers in nasal secretions.

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“…published by Worrell et al [21], ''An integrated all foil based micro device for point of care diagnostic applications.'' published by Bose et al [22], and ''An innovative tool for moving malaria PCR detection of parasite reservoir into the field.'' published by Canier et al [23], they all adopted the POCT microdevice to detect DNA fragments of certain microbe.…”

Section: Introductionmentioning

confidence: 99%

A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection

Shi

et al. 2020

IEEE Access

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Polymerase chain reaction (PCR) technology is an essential technology for point-of-care testing (POCT) application. To realize the simple, portable and fast detection of bacteria has always been an important technology. In this paper, we developed a pipeline-based oil-bath PCR method, to achieve the PCR amplification and detection for bacteria. We assembled a small-sized system with a uniform temperature environment and a stable heating module. By comparing the PCR performance with the other two existing pipeline-based methods on the reactions of both DNA fragment of H7N9 avian influenza and Escherichia Coli, the results show that the pipeline-based oil-bath PCR method is fully capable of the amplification and detection of both DNA fragment and bacteria. At the meanwhile, we performed two sets of tests, the experiment of pipeline diameter change and bacterial concentration gradient, to verify the sensitivity and universality of this method. The results show that the pipeline-based oil-bath PCR method developed by us not only gets rid of the complicated preparations, offers advantages of simple operation, low-cost and user-friendly, but also provides a stable and portable foundation for our later work.

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An integrated all foil based micro device for point of care diagnostic applications

Cited by 5 publications

References 18 publications

3D Printing: An Alternative Microfabrication Approach with Unprecedented Opportunities in Design

3D Printing: An Alternative Microfabrication Approach with Unprecedented Opportunities in Design

Flow-Based CL-SMIA for the Quantification of Protein Biomarkers from Nasal Secretions in Comparison with Sandwich ELISA

A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection

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