2023
DOI: 10.1016/j.bios.2023.115245
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An integrated and flexible PDMS/Au film-based electrochemical immunosensor via Fe–Co MOF as a signal amplifier for alpha fetoprotein detection

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Cited by 31 publications
(8 citation statements)
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“…Table 1 summarizes the comparison between AFP detection performance using different electrodes through photoelectrochemical (PEC), electrochemical (EC), or electrochemiluminescence (ECL) detection [43][44][45][46][47][48]. The LOD of the fabricated sensor is lower than that obtained using the AFP antibody/conjugated polymers/titanium dioxide/fluorine-doped tin oxide conductive glass electrode (Ab/CP/TiO 2 /FTO) [43], sandwich analysis based on poly-(2-hydroxyethyl methacrylate)-g-graphene/the second antibody/AFP/BSA/the first antibody/gold nanoparticle-reduced graphene oxide-modified GCE (P(VT-co-HEMA)-g-GO/Ab 2 /AFP/BSA/Ab 1 /AuNPs-rGO/GCE) [44], metal-organic framework-antibody/ AFP/BSA/aptamer-SH/gold nanoparticle/flexible polydimethylsiloxane-modified GCE (MOF-Ab/AFP/BSA/Apt-SH/Au/PDMS/GCE) [45], or antibody/carbodiimide hydrochloride-N-hydroxysuccinimide/ferroferric oxide/carboxylated carbon nanotubes/gold nanoparticlemodified GCE (Ab/EDC-NHS/Fe 3 O 4 /MWCNTs-COOH/AuNPs/GCE) [46] but higher than that obtained on AFP/BSA/AFP antibody/reduced graphene oxide@gold nanoparticle @Ru(bpy) 3 2+ -doped silica nanoparticle-modified GCE (AFP/BSA/Ab/rGO@Au@Ru-SiO2/GCE) [47] and fluorescent microspheres based on 4-vinylphenylboronic acid and divinylbenzene-AFP/AFP antibody/Ti, N/Mg co-doped carbon dot-modified GCE (Poly (DVB-Co-PBA)-AFP/Ab/Ti,Mg@N-CDs/GCE) [48]. Compared with other electrodes that need antibody/DNA to be fixed at the recognition interface or sandwich detection modes, the homogeneous electrochemical sensor constructed by this method has the advantages of simple preparation and high sensitivity.…”
Section: Electrochemical Determination Of Afp Using the Fabricated Ho...mentioning
confidence: 99%
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“…Table 1 summarizes the comparison between AFP detection performance using different electrodes through photoelectrochemical (PEC), electrochemical (EC), or electrochemiluminescence (ECL) detection [43][44][45][46][47][48]. The LOD of the fabricated sensor is lower than that obtained using the AFP antibody/conjugated polymers/titanium dioxide/fluorine-doped tin oxide conductive glass electrode (Ab/CP/TiO 2 /FTO) [43], sandwich analysis based on poly-(2-hydroxyethyl methacrylate)-g-graphene/the second antibody/AFP/BSA/the first antibody/gold nanoparticle-reduced graphene oxide-modified GCE (P(VT-co-HEMA)-g-GO/Ab 2 /AFP/BSA/Ab 1 /AuNPs-rGO/GCE) [44], metal-organic framework-antibody/ AFP/BSA/aptamer-SH/gold nanoparticle/flexible polydimethylsiloxane-modified GCE (MOF-Ab/AFP/BSA/Apt-SH/Au/PDMS/GCE) [45], or antibody/carbodiimide hydrochloride-N-hydroxysuccinimide/ferroferric oxide/carboxylated carbon nanotubes/gold nanoparticlemodified GCE (Ab/EDC-NHS/Fe 3 O 4 /MWCNTs-COOH/AuNPs/GCE) [46] but higher than that obtained on AFP/BSA/AFP antibody/reduced graphene oxide@gold nanoparticle @Ru(bpy) 3 2+ -doped silica nanoparticle-modified GCE (AFP/BSA/Ab/rGO@Au@Ru-SiO2/GCE) [47] and fluorescent microspheres based on 4-vinylphenylboronic acid and divinylbenzene-AFP/AFP antibody/Ti, N/Mg co-doped carbon dot-modified GCE (Poly (DVB-Co-PBA)-AFP/Ab/Ti,Mg@N-CDs/GCE) [48]. Compared with other electrodes that need antibody/DNA to be fixed at the recognition interface or sandwich detection modes, the homogeneous electrochemical sensor constructed by this method has the advantages of simple preparation and high sensitivity.…”
Section: Electrochemical Determination Of Afp Using the Fabricated Ho...mentioning
confidence: 99%
“…GCE (MOF-Ab/AFP/BSA/Apt-SH/Au/PDMS/GCE) [45], or antibody/carbodiimide hydrochloride-N-hydroxysuccinimide/ferroferric oxide/carboxylated carbon nanotubes/gold nanoparticle-modified GCE (Ab/EDC-NHS/Fe3O4/MWCNTs-COOH/AuNPs/GCE) [46] but higher than that obtained on AFP/BSA/AFP antibody/reduced graphene oxide@gold nanoparticle@Ru(bpy)3 2+ -doped silica nanoparticle-modified GCE (AFP/BSA/Ab/rGO@Au@Ru-SiO2/GCE) [47] and fluorescent microspheres based on 4-vinylphenylboronic acid and divinylbenzene-AFP/AFP antibody/Ti, N/Mg co-doped carbon dot-modified GCE (Poly(DVB-Co-PBA)-AFP/Ab/Ti,Mg@N-CDs/GCE) [48]. Compared with other electrodes that need antibody/DNA to be fixed at the recognition interface or sandwich detection modes, the homogeneous electrochemical sensor constructed by this method has the advantages of simple preparation and high sensitivity.…”
Section: Electrochemical Determination Of Afp Using the Fabricated Ho...mentioning
confidence: 99%
“…24 For example, our group previously used Fe–Co MOF to act as a signal amplifier due to its peroxidase-like activity to establish an integrated electrochemical immunosensor for the detection of alpha fetoprotein (AFP) based on an Au/polydimethylsiloxane (PDMS) flexible film electrode. 25 A Cu MOF was revealed to have an excellent peroxidase activity, and a bimetallic MOF with Cu and Ni intercalation exhibited better catalytic effects than the single MOF. Therefore, the bimetallic Cu–Ni MOF can be applied for signal amplification in the construction of electrochemical immunoassay sensors.…”
Section: Introductionmentioning
confidence: 98%
“…7–10 In recent years, a growing number of researchers devoted themselves to exploiting new and effective electrode modification materials with good catalytic activity and electrical conductivity to obtain high performance electrochemical sensors. 11–13 Metal nanoparticles (MNPs) such as gold (Au), silver (Ag) and copper (Cu), as a hotspot, have evoked tremendous research interest due to their unique physicochemical properties, high catalytic activity and excellent electrical conductivity. 14–16 In addition, gold nanoparticles (Au NPs) and silver nanoparticles (Ag NPs) possess strong affinity towards thiols, which is conducive to covalency bind thiol-modified DNA/RNA on the electrode surface.…”
Section: Introductionmentioning
confidence: 99%